Preparation method and application of fused bovine antibacterial peptide and interleukin 2 co-expression recombinant yeast preparation

Abstract

The invention discloses a preparation method and an application of an antibacterial peptide fused cytokine FBAPIL2 co-expression biological preparation. The antibacterial peptide fused cytokine FBAPIL2 is a protein A1) with the amino acid sequence represented by sequence 1, or a protein A2) obtained by substituting and / or deleting and / or adding one or more amino acid residues to an amino acid sequence represented by a sequence 1 in a sequence table and having same functions, or a fusion protein A3) obtained through connecting a label to the N end or / and the C end of the protein A1) or A2). Experiments prove that the FBAPL2 can promote the proliferation of lymphocytes, erythrocyte and leukocytes, inhibit the growth of pathogenic microorganisms, promote the secretion of non-specific antibodies (IgG, IgG1 and IgG2a) and disease-specific antibodies and improve the immunity and survival rate of animals.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the preparation and application of a recombinant yeast preparation fused with bovine antimicrobial peptide and interleukin-2 co-expression. Background technique [0002] The aquaculture industry (animal husbandry and aquatic products) is an important part of large-scale agriculture. It is one of the important foundations for my country's agricultural modernization, rural economic development, and social stability. The aquaculture industry in advanced countries in Europe and the United States is very developed, accounting for 50% of large-scale agriculture. % above the specific gravity. It provides human beings with meat, milk, eggs, fur and other high-grade foods and important textile raw materials. Animal products are the main source of high-quality protein, minerals, vitamins and other nutrients for people. Their safety is directly related to human health. In recent yea...

AI Technical Summary

Problems solved by technology

The abuse of feed antibiotic additives not only increases the cost of feeding, but also leads to a significant increase in the drug resistance and pathogenicity of pathogenic microorganisms, and the health of livestock and poultry bodies is weakened due to the side effects of drugs, and the immunity and disease resistance are significantly reduced

In such a vicious cycle, various severe infectious diseases of livestock and poultry frequently occur, making it difficult to control and treat

On the other hand, drug residues and the increasingly serious food safety problems of livestock and poultry products not only directly threaten the health of human beings themselves, but also hinder the development of the breeding industry.

[0006] The addition of antibiotics to animal feed, long-term or inappropriate use will not only induce drug resistance in bacteria, but also cause drug residues in animal products, causing serious food safety hazards, damaging human health and inducing the failure of drugs used to treat antibiotics

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