Preparation and application of bacillus amyloliquefaciens gy30 and its powder
A technology of amylolytic and bacillus, applied to the direction of bacteria fixed on or in inorganic carriers, fixed on/in organic carriers, etc., which can solve the problems of poor adaptability, high culture temperature, and few good strains in production And other issues
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Embodiment 1
[0276] Example 1, a strain of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) GY30 derived from the ocean, its preservation number is: CGMCC NO.14480.
[0277] The spore-producing fermentation culture method of the described bacillus amyloliquefaciens GY30 bacterial strain, its steps are as follows:
[0278] (1) Inoculate the GY30 strain on the PDA medium for activation culture, and pick the GY30 strain with an inoculation loop under sterile conditions in an incubator equipped with a seed liquid medium, 28°C, 180r / min shaking culture; adjust the bacteria Liquid concentration is 10 9 cells / mL, as a primary seed liquid; the formula of the seed liquid culture medium is: glucose 20g / L, potato 200g / L, water 1L;
[0279] (2) Inoculate the primary seed liquid into a 50L fermenter equipped with 30L fermentation medium according to the inoculum amount of 6%, ferment and cultivate at 32°C and 180r / min for 12-14h, and prepare the secondary seed liquid. The formula of the fermen...
Embodiment 2
[0287] Embodiment 2, utilize the bacillus amyloliquefaciens GY30 bacterial strain described in embodiment 1 to prepare the method for GY30 bacterium powder, its steps are as follows: the bacillus amyloliquefaciens GY30 bacterial strain is carried out producing spores by the method described in claim 2 in fermenter Fermenting and cultivating, the obtained fermented liquid is spray-dried with bentonite as a drying agent to obtain GY30 bacterial powder. The amount of bentonite is 7% of the fermentation broth, and the temperature of the air entering the spray tower is 160° C. during spray drying.
[0288] The prepared GY30 bacterium powder can be used as an active ingredient to prepare medicine or pharmaceutical composition for preventing and treating rice sheath blight.
Embodiment 3
[0289] Embodiment 3, utilize the method for preparing the GY30 bacterium powder of bacillus amyloliquefaciens GY30 bacterial strain described in embodiment 1, its steps are as follows: the bacillus amyloliquefaciens GY30 bacterial strain is carried out spore production by the method described in claim 2 in fermentor Fermenting and cultivating, the obtained fermented liquid is adsorbed and dried with rice husk powder as a carrier until the water content is lower than 10%, and passed through a 100-mesh sieve to obtain GY30 bacterial powder. The mass volume ratio of the rice husk powder to the fermented liquid is 1:1.
[0290] The prepared GY30 bacterial powder can be used as an active ingredient to prepare rice microbial fertilizers.
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