Industrial high-density mixed fermentation culture medium of bifidobacterium and lactobacillus, fermentation culture method and fungus powder embedding method

Abstract

The invention provides an industrial high-density mixed fermentation culture medium of bifidobacterium and lactobacillus, a fermentation culture method and a fungus powder embedding method. The fermentation culture medium is prepared from the following components: 0.3 to 1.5 percent of corn slurry dry powder, 0 to 1.8 percent of soya peptone, 0 to 1 percent of fructooligosaccharide, 0.5 to 1 percent of glucose, 0.4 to 1.35 percent of dipotassium phosphate, 0.4 percent of potassium dihydrogen phosphate, 0.015 to 0.02 percent of magnesium sulfate, and 0.05 percent of L-cysteine hydrochloride; orthe fermentation culture medium is prepared from the following components: 0.3 to 1.5 percent of the corn slurry dry powder, 0 to 1 percent of beef extract, 0 to 1 percent of the fructooligosaccharide, 0.5 to 1 percent of the glucose, 0.4 to 1.35 percent of the dipotassium phosphate, 0.4 percent of the potassium dihydrogen phosphate, 0.015 to 0.02 percent of the magnesium sulfate and 0.05 percentof the L-cysteine hydrochloride; the fermentation method comprises strain activation and culture, first-grade strain culture, second-grade seed solution preparation, 200L small tank culture in a workshop and 1T large tank culture in the workshop. Comparing the relatively traditional TPY culture medium, the viable count improves 10 times or more and the cost is reduced by 60 percent, meanwhile, according to the formula, raw materials are easy to obtain and the components are simple, so that the fermentation culture medium is suitable for industrial production requirements. The fungus powder embedding method is adopted so that the human body and external environment tolerance capability of strains is greatly improved.

Description

technical field [0001] The invention belongs to the technical field of probiotic product preparation, and relates to an industrialized high-density mixed fermentation medium of bifidobacteria and lactobacilli, a fermentation culture method and a bacteria powder embedding method. Background technique [0002] Probiotics are a class of active microorganisms that are beneficial to the host. They colonize the human intestinal tract and reproductive system and can produce definite health effects, thereby improving the host's micro-ecological balance and exerting beneficial effects. The general term for active microorganisms. There are a huge number of microorganisms inhabiting the human gut, and their composition and activities affect the host's immunity, mucosal development, and metabolism of nutrients and drugs. As one of the main types of probiotics, lactic acid bacteria play an important role in maintaining the balance of intestinal flora, reducing cholesterol, reducing endot...

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems of high medium cost, complex industrial production method and low in vitro survival rate of bacterial powder in the current co-cultivation of probiotics, and at the same time increase the viable count of lactic acid bacteria in industrial production, the invention provides a direct-injection dual The high-density mixed fermentation medium, fermentation culture method and large-scale production fermentation method of Mycobacterium and Lactobacillus, through screening and optimizing different culture media and culture methods, and taking some comprehensive measures such as simple and practical metabolic regulation methods, make the culture medium The density of lactic acid bacteria reaches 10 10 cfu / ml

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