Industrial high-density mixed fermentation culture medium of bifidobacterium and lactobacillus, fermentation culture method and fungus powder embedding method
A bifidobacterium and lactobacillus technology, applied in the direction of bifidobacteria, microbial-based methods, biochemical equipment and methods, etc., can solve complex industrial production methods, high cost of culture medium, low in vitro survival rate of bacterial powder, etc. problems, to achieve the effect of easy purchase, simple ingredients, and improved survivability
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[0062] Example 1, Screening of high-density fermentation medium for mixed strains:
[0063] 1. The influence of different carbon and nitrogen sources on the number of viable bacteria in the fermentation of mixed strains:
[0064] According to the analysis of the formula of lactobacilli and bifidobacteria in the current workshop and literature, the main components of the culture medium are glucose, lactose, oligofructose, peptone, soy peptone, yeast powder, beef extract, and the 7-factor 2-level orthogonal test , Orthogonal out the results of the 7-factor percentage content of serial numbers 1-8, and weigh the serial numbers 1-8 according to the percentage content of the 7 factors, and then add the basic medium components, the basic medium components are: dipotassium hydrogen phosphate 1.35%, sulfuric acid Magnesium 0.02%, Tween 0.1%, L-cysteine hydrochloride 0.05%, adjust pH 6.8-7.0, prepare 8 kinds of culture media in 500mL saline bottle, lactic acid bacteria culture broth and b...
Example Embodiment
[0081] Example 2, a direct-cast high-density mixed culture and mass production fermentation method of bifidobacteria and lactobacilli, the method includes the following steps:
[0082] Step one, activated culture of strain:
[0083] Adjust the pH value of MRS and TPY slant medium with 6mol / L sodium hydroxide to 6.5, sterilize at 121℃ for 15min, and then use them; before the strain is activated, store the Lactobacillus plantarum and growth medium at -80℃. Place the ampoules of bifidobacteria at room temperature for 2 hours, then wipe the surface of the ampoules with a 75% alcohol cotton ball in a sterile operating table, open the ampoules, and use a sterilized inoculation loop to pick 2-3 loops evenly. Line, inoculate Lactobacillus plantarum on MRS slant medium, and Bifidobacterium longum on TPY slant medium, respectively cultivate under anaerobic conditions at 30℃ for 48h, terminate the culture, check the morphology of the bacteria under microscope, and continue after no pollution ...
Example Embodiment
[0102] Embodiment 3, a direct-cast high-density mixed culture and large-scale fermentation method of bifidobacteria and lactobacilli, the method includes the following steps:
[0103] Step one, activated culture of strain:
[0104] Adjust the pH value of the MRS and TPY slant medium to 7.0 respectively, and sterilize them at 121°C for 30 minutes before use; before the strain is activated, store the Lactobacillus rhamnosus and Bifidobacterium lactis at -80°C. Place the ampoules at room temperature for 3 hours, then wipe the surface of the ampoules with a 75% alcohol cotton ball in a sterile operating table, open the ampoules, pick 2-3 loops with a sterile inoculation loop, and scribe them evenly. Inoculate Lactobacillus Leesose on MRS slant medium and Bifidobacterium lactis on TPY slant medium. Cultivate them at 37°C for 24 hours under anaerobic conditions, terminate the culture, check the morphology of the bacteria microscopically, and activate continuously after contamination-free...
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