Lycium ruthenicum culture medium and preparation method thereof
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A culture medium, technology of black wolfberry, applied in the direction of culture medium, botany equipment and method, planting substrate, etc., can solve the problems of low reproduction rate and slow reproduction speed, and achieve easy-to-obtain ingredients, easy operation and reasonable formula composition Effect
Inactive Publication Date: 2018-07-31
康美药业(昆明)种质资源有限公司
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Problems solved by technology
And existing black wolfberry, propagation speed is slow, and black wolfberry stem, leaf reproduction rate is low under natural environment, can't satisfy people's demand far away
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preparation example Construction
[0038] A preparation method of black wolfberry culture medium, comprising:
[0039] The preparation steps of the mother liquor of macroelements: respectively take the remaining macroelement components except calcium chloride dihydrate, mix them, and prepare the mother liquor of macroelements, the concentration of each component in the mother liquor of macroelements is 10 times of the concentration of their respective formulas; Calcium chloride is prepared separately, and the concentration of calcium chloride dihydrate solution is 10 times that of the formula;
[0040] Trace element mother liquor preparation steps: take trace element components respectively, mix, and prepare trace element mother liquor, the concentration of each component in the trace element mother liquor is 100 times of the respective formula concentration;
[0041] Preparation steps of iron salt mother liquor: prepare ferrous sulfate solution and disodium edetate solution respectively, then mix, adjust pH an...
Embodiment 1-5
[0056] A kind of black wolfberry culture medium, comprises macroelement component (potassium nitrate, ammonium nitrate, calcium chloride dihydrate, magnesium sulfate heptahydrate and potassium dihydrogen phosphate), trace element component (potassium iodide, boric acid, manganese sulfate, heptahydrate Zinc sulfate, sodium molybdate, copper sulfate pentahydrate and cobalt chloride), iron salt components (ferrous sulfate and disodium edetate), organic components (niacin, pyridoxine hydrochloride, thiamine hydrochloride and glycine), phytohormone components (2,4-dichlorophenylacetic acid, α-naphthaleneacetic acid, and 6-benzylaminopurine), inositol, agar powder, and sucrose, and the pH of the medium was adjusted with an acid-base indicator to 5.8-6.0. The concrete formula table of embodiment 1-5 is shown in following table 1.
[0057] The formula table of the black wolfberry medium of table 1 embodiment 1-5
[0058]
[0059]
[0060] The black wolfberry culture medium of ...
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Abstract
The invention discloses a lycium ruthenicum culture medium. The culture medium comprises macro-element components, trace-element components, iron salt components, organic components, plant hormone components, inositol, agar powder and sucrose; the macro-element components comprise potassium nitrate, ammonium nitrate, calcium chloride dihydrate, magnesium sulfate heptahydrate and potassium dihydrogen phosphate; the micro-element components comprise potassium iodide, boric acid, manganese sulfate, zinc sulfate heptahydrate, sodium molybdate, copper sulfate pentahydrate and cobalt chloride; the iron salt components comprise ferrous sulfate and disodium ethylenediamine tetraacetic acid; the organic matter components comprise nicotinic acid, pyridoxine hydrochloride, thiamin hydrochloride and glycine; and the plant hormone components comprise 2,4-dichlorophenylacetic acid, alpha-naphthylacetic acid and 6-benzylaminopurine. The culture medium can enable the stems and leaves of the lycium ruthenicum to differentiate under an aseptic environment, induce the growth of the seedlings and solve the problem of low reproductive rate of stems and leaves in a natural environment. The invention also discloses a preparation method of the lycium ruthenicum culture medium; the process is simple; the operation is simple and convenient; and the manufacturing cost is low.
Description
technical field [0001] The invention relates to the technical field of plant tissue culture and seedling cultivation, in particular to a black wolfberry culture medium and a preparation method thereof. Background technique [0002] Black wolfberry (Latin name Lycium ruthenicum Murr), also known as sweet wolfberry. Distributed in northern Shaanxi, Ningxia, Gansu, Qinghai, Xinjiang and Tibet; also in Central Asia, Caucasus and Europe. Drought-resistant, it often grows in saline-alkaline soil wasteland, sandy land or roadsides. Black wolfberry can be used as a shrub for soil and water conservation, thorny shrub, 20-150 cm high, multi-branched; branches ascending or lying on the ground, white or off-white, hard, often zigzagging, with irregular vertical stripes , the tops of branchlets gradually become thorns, the internodes are shortened, and each node has short thorns with a length of 0.3-1.5 cm; the short branches are located on both sides of the thorns, which are not obvio...
Claims
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Application Information
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