A human stomach-small intestine digestion simulation method and device

A simulation method and technology of a simulation device, which are applied in the field of bionics, can solve the problems of not considering the dynamic changes of intestinal physical and chemical conditions, difficult to popularize, and not considering the dynamic release of dynamic transport of digestive juice.

Active Publication Date: 2020-04-14
JIANGNAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These two simulation devices are very useful, but the stomach and small intestine part of this system is also a static digestion model, which does not consider the dynamic transport of contents in the stomach and small intestine and the dynamic release of digestive juice. Laboratory popularization
[0004] In summary, there are still some limitations in the existing research involving human digestion simulation: 1) the dynamic changes of intestinal physicochemical conditions, especially the real-time dynamic changes of gastric pH; The real-time flow between them, including the process of gastric emptying and intestinal transit; 3) The corresponding parameters were not adjusted according to the characteristics of the population, including premature infants, full-term infants, adults and the elderly, etc.

Method used

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  • A human stomach-small intestine digestion simulation method and device
  • A human stomach-small intestine digestion simulation method and device
  • A human stomach-small intestine digestion simulation method and device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: Dynamic Analysis of Digestion of Breast Milk Triglycerides in Premature Infants

[0037] Solution preparation:

[0038] Artificial gastric juice: Weigh 8,600U of rabbit gastric lipase and 120,000U of pig gastric mucosal pepsin into 100mL gastric electrolyte solution. Preparation of gastric electrolyte solution: Weigh 6.9gNaCl, 0.73gKCl, 0.12 gMgCl 2 (H 2 O) 6 , 0.05gNH 4 Cl, 0.01g urea and 0.1g CaCl 2 (H 2 O) 2 , dissolved in 800mL deionized water, adjusted to pH 6.5 with 0.15M HCl, and finally dilute to 1L. Store in refrigerator at 4°C.

[0039] Artificial pancreatic juice: Weigh a certain amount of porcine pancreatic enzyme (including trypsin, chymotrypsin and pancreatic lipase), so that the enzyme activity is 5900U of porcine pancreatic lipase and add it to 100mL small intestine electrolyte solution. Preparation of small intestine electrolyte solution: weigh 8.2gNaCl , 4.6gCaCl 2 , 0.3gKCl, 0.06g0.12gMgCl2 6H 2 O, 0.11g urea and 0.2g CaCl 2 (H ...

Embodiment 2

[0046] Example 2: Dynamic Analysis of Protein Digestion in the Elderly

[0047] Solution preparation:

[0048] Artificial gastric juice: Weigh 8,400U of rabbit gastric lipase and 75,000U of pig gastric mucosal pepsin into 100mL gastric electrolyte solution. Preparation of gastric electrolyte solution: Weigh 6.9gNaCl, 0.73gKCl, 0.12 gMgCl 2 (H 2 O) 6 , 0.05gNH 4Cl, 0.01g urea and 0.1g CaCl 2 (H 2 O) 2 , dissolved in 800mL deionized water, adjusted to pH 6.5 with 0.15M HCl, and finally dilute to 1L. Store in refrigerator at 4°C.

[0049] Artificial pancreatic juice: Weigh a certain amount of porcine pancreatin (containing trypsin, chymotrypsin and pancrelipase), make the enzyme activity 4600U trypsin and add it to 100mL small intestine electrolyte solution, the preparation of small intestine electrolyte solution: weigh 8.2gNaCl, 4.6gCaCl 2 , 0.3gKCl, 0.06g0.12gMgCl2 6H 2 O, 0.11g urea and 0.2g CaCl 2 (H 2 O) 2 , dissolved in 800ml of deionized water, adjusted to pH...

Embodiment 3

[0056] Embodiment 3: the contrast of dynamic (the present invention method) and static (prior art method) model simulation bovine milk fat gastric digestion

[0057] The simulation process adopts the human stomach-small intestine digestion simulation device of the present invention, the gastric juice reagent bottle 101a-1, the hydrochloric acid reagent bottle 101a-2 and the sodium hydroxide reagent bottle 101a-3 pass through the peristaltic pump 101b-1~101b-4 and silica gel The tube is connected to the simulated gastric digestion chamber 102a; the pancreatic juice reagent bottle 201a-1, the bile reagent bottle 201a-2, the hydrochloric acid reagent bottle 101a-2 and the sodium hydroxide reagent bottle 101a-3 pass through the peristaltic pump 201b-1~201b-4 and silica gel The tube is connected to the simulated small intestine digestion chamber 202a; the gastric digestion chamber 102a is connected to the simulated small intestine digestion chamber 202a through the peristaltic pump ...

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Abstract

The invention discloses a human body stomach-small intestine digestion simulation method and device. According to the invention, real time dynamic control over pH values of the stomach and a small intestine digestion chamber is realized and pH dynamic curve can be output at the same time. Through regulating stomach emptying speed and intestine transport speed in real time, real time flow of food between different digestive tracts is realized. Human body stomach-small intestine digestion state and environment are simulated more truly and more comprehensively. According to the invention, simulation of an external digestion process of different people such as premature infants, term infants, adults and aged people can be realized. Rabbit gastric lipase or cutinase are used in simulated gastric fluid, so that human gastric lipase is simulated more truly.

Description

technical field [0001] The invention belongs to the technical field of bionics, and relates to a human gastrointestinal tract digestion simulation device and a simulation experiment method based on the device, in particular to a simulating human gastrointestinal tract environmental conditions and food digestion process under in vitro conditions and a system based on the system Methods for simulating digestion. Background technique [0002] Digestion of food and drugs in the human gastrointestinal tract is a complex process involving chemical, enzymatic and mechanical actions. The digestive process determines food availability, satiety generation, drug release efficiency, microbial viability or inactivation, and foodborne toxicant transformation and release. Therefore, the evaluation of the digestion process of food and drugs has a wide application value in basic research and product development in the fields of nutrition, food science, microbiology, pharmacology, toxicology...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G09B23/28
CPCG09B23/28
Inventor 孙进徐亚华齐策王兴国金青哲余任强
Owner JIANGNAN UNIV
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