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Application of caffeic acid phenethylester in preparing of caries-prevention or anti-caries preparation

A technology of phenethyl caffeate and preparations, which is applied in the field of caries prevention and treatment, can solve the problem of no research and reports on the anti-caries effect, and achieve the effect of preventing tooth demineralization, low production cost and single ingredient

Active Publication Date: 2018-08-17
四川护家卫士生物医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] However, there is no research or report on whether phenethyl caffeate still has the anti-caries effect of propolis and its polyphenol extract as a single active ingredient

Method used

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  • Application of caffeic acid phenethylester in preparing of caries-prevention or anti-caries preparation
  • Application of caffeic acid phenethylester in preparing of caries-prevention or anti-caries preparation
  • Application of caffeic acid phenethylester in preparing of caries-prevention or anti-caries preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1 Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) Determination

[0041] The minimum inhibitory concentration MIC (Minimal inhibitory concentration) refers to the lowest concentration of antibacterial drugs that can inhibit the growth of bacteria in the medium in vitro tests. Minimal bactericidal concentration MBC (Minimal bactericidal concentration) refers to the minimum concentration of antibacterial drugs that can kill viable bacteria in the medium in vitro tests. MIC and MBC are indicators of drug antibacterial activity, showing the ability of drugs to inhibit and kill pathogenic microorganisms. The bacteria used in the experiment are common oral cariogenic bacteria: Streptococcus mutans UA159, Streptococcus sobrinus 6715, Streptococcus sanguinis ATCC10556, Actinomyces viscosus ATCC15987, acidophilus Bacillus (Lactobacillus acidophilus ATCC14931) was provided by the State Key Laboratory of Oral Diseases Research, and w...

Embodiment 2

[0057] The mensuration of embodiment 2 sterilization curves

[0058] Bactericidal kinetics is an index reflecting the relationship between drug antibacterial ability and action time. The bacteria used in the experiment were the main oral cariogenic bacteria, Streptococcus mutans (Streptococcus mutans UA159). Chlorhexidine (CHX) was used as a positive control, 5% DMSO was used as a solvent control, and sterile deionized water was used as a negative control.

[0059] The experimental steps are as follows:

[0060] 1. Pick a single colony and culture overnight at 37°C in 10mL BHI liquid medium.

[0061] 2. Take 100 μL of bacterial solution and culture it in 10ml BHI liquid medium for 6 hours, dilute the bacterial solution to 2×10 6 CFU / mL for use.

[0062] 3. Phenylethyl caffeate was dissolved and diluted with 50% DMSO and added to a 24-well plate, 200 μL per well.

[0063] 4. Add 800 μL of BHI medium and 1 mL of spare bacterial solution to each well, so that the final conce...

Embodiment 3

[0069] The impact of embodiment 3 caffeic acid phenethyl ester on streptococcus mutans biofilm formation

[0070] 3.1 Caffeic acid phenethyl ester MBIC 50 determination

[0071] The minimum inhibitory biofilm concentration MBIC (Minimal biofilm inhibitory concentration) is an index reflecting the anti-biofilm formation ability of drugs. Among them MBIC 50 It refers to the lowest concentration of antibacterial drugs that can inhibit more than 50% of the biofilm formation in the medium in the in vitro test. Streptococcus mutans UA159 was used in the experiment, sterile deionized water was used as a negative control, and 5% DMSO was used as a solvent control.

[0072] The experimental steps are as follows:

[0073] 1. Pick a single colony and put it in a 37°C constant temperature anaerobic culture tank (80%N) in 10mL BHI liquid medium 2 , 10%H 2 , 10% CO 2 ) overnight culture.

[0074] 2. Take 100 μL of the bacterial solution and place it in a constant temperature anaerob...

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Abstract

The invention discloses application of caffeic acid phenethylester, a polyphenol component in natural propolis, in preparing of a caries-prevention and anti-caries preparation. The caffeic acid phenethylester has bacteria-inhibiting and bactericidal effects on various cariogenic bacteria including streptococcus mutans, streptococcus sobrinus, streptococcus sanguis, actinomyces viscosus, and lactobacillus acidophilus, can also effectively inhibit formation of a streptococcus mutans biofilm, and reduce the metabolic capacity of a formed biofilm, and has the capability of reducing the amount of lactic acid produced by the streptococcus mutans and reducing the ability to produce exopolysaccharides, thereby achieving caries-prevention and anti-caries functions in various aspects. In addition, and compared with anti-caries propolis, the caffeic acid phenethylester has the advantages that the composition is single, the curative effect is exact, the cost is low, and the caffeic acid phenethylester is easy to popularize and apply in dental care.

Description

technical field [0001] The invention belongs to the technical field of caries prevention and treatment, and in particular relates to the application of phenethyl caffeate in preparing anti-caries or anti-caries preparations. Background technique [0002] Caries is a disease in which the hard tissue of the tooth body is chronically and progressively destroyed under the influence of multi-factors mainly composed of bacteria. Caries is the most common disease in the human oral cavity. The World Health Organization (WHO) has listed caries as one of the key human diseases. A large amount of evidence shows that the existence of bacteria is a prerequisite for the occurrence of caries. Surface colonization forms a "biofilm", and metabolizes carbohydrates to produce acid, causing demineralization of dental hard tissues and forming cavities. Among them, Streptococcus mutans is the main cariogenic bacteria. The glucosyltransferase it produces catalyzes sucrose to produce glucan to med...

Claims

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Application Information

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IPC IPC(8): A61K31/216A61P1/02A23L33/10
CPCA23V2002/00A61K31/216A23L33/10A61P1/02A23V2200/312
Inventor 张凌琳牛玉梅李莹雪王雨霏
Owner 四川护家卫士生物医药科技有限公司
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