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Sartorya Vuill W-10 with high cellulase yield as well as fermenting method and application thereof

A technology of cellulase and Aspergillus, applied in the field of fermentation and production of cellulase, can solve the problems of increasing production costs and affecting the economic benefits of enterprises, and achieve the effects of increasing production, facilitating commercial application and implementation, and high ethanol yield

Inactive Publication Date: 2018-08-17
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, most of the existing fungi that produce cellulase are compound bacterial agents, and sometimes cellulase needs to be added in the production process to improve the enzymatic hydrolysis rate of lignocellulose, thereby increasing the final ethanol yield. Increase production costs and affect the economic benefits of enterprises

Method used

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  • Sartorya Vuill W-10 with high cellulase yield as well as fermenting method and application thereof
  • Sartorya Vuill W-10 with high cellulase yield as well as fermenting method and application thereof
  • Sartorya Vuill W-10 with high cellulase yield as well as fermenting method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The specific preparation process of cellulase prepared by adopting Aspergillus mycelium (Sartorya Vuill W-10) is as follows:

[0045] (1) Preparation of culture medium: Preparing according to potassium nitrate 4g, lactose 4g, urea 0.2g, dipotassium hydrogen phosphate 1.5g, Mandel's nutrient solution 3ml, bran 6g, magnesium sulfate 0.5g, calcium chloride 0.3g, sodium hydroxide The treated straw powder was prepared at a ratio of 30g to obtain a medium, and the volume was adjusted to 1000mL with deionized water, and the pH value of the medium was adjusted to 3 with 1mol / L hydrochloric acid; then, according to the bottled volume of 43mL / 250mL, the prepared The culture medium is divided into the fermentation vessel;

[0046] (2) Inoculation of Aspergillus miliflora seed liquid: according to 10% of the volume of the culture medium, inoculate the Aspergillus miliflora seed liquid into a fermentation container filled with culture medium;

[0047] (3) Preparation of cellulase: ...

Embodiment 2

[0050] The specific preparation process of cellulase prepared by adopting Aspergillus mycelium (Sartorya Vuill W-10) is as follows:

[0051] (1) Preparing the culture medium: 4g of potassium nitrate, 4g of lactose, 0.2g of urea, 1.5g of dipotassium hydrogen phosphate, 6ml of Mandel's nutrient solution, 12g of bran, 0.5g of magnesium sulfate, 0.3g of calcium chloride, sodium hydroxide The treated straw powder was prepared at a ratio of 30g to obtain a medium, and the volume was adjusted to 1000mL with deionized water, and the pH value of the medium was adjusted to 2.5 with 1mol / L hydrochloric acid; then, according to the bottling volume of 60mL / 250mL, the The culture medium is divided into the fermentation vessel;

[0052] (2) Inoculation of Aspergillus miliformis seed liquid: according to 8% of the medium volume, inoculate the Aspergillus militicum seed liquid in a fermentation vessel filled with medium;

[0053] (3) Preparation of cellulase: ferment the culture inoculated wi...

Embodiment 3

[0056] The specific preparation process of cellulase prepared by adopting Aspergillus mycelium (Sartorya Vuill W-10) is as follows:

[0057] (1) Preparation of culture medium: Preparing according to potassium nitrate 4g, lactose 4g, urea 0.2g, dipotassium hydrogen phosphate 1.5g, Mandel's nutrient solution 2ml, bran 4g, magnesium sulfate 0.5g, calcium chloride 0.3g, sodium hydroxide The treated straw powder was prepared at a ratio of 30g to obtain a medium, and the volume was adjusted to 1000mL with deionized water, and the pH value of the medium was adjusted to 3.5 with 3mol / L hydrochloric acid; then, according to the bottled volume of 40mL / 250mL, the prepared The culture medium is divided into the fermentation vessel;

[0058] (2) Inoculation of Aspergillus miliformis seed liquid: according to 15% of the volume of the culture medium, inoculate the Aspergillus miliformis seed liquid in a fermentation container filled with medium;

[0059] (3) Preparation of cellulase: Fermen...

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Abstract

The invention discloses Sartorya Vuill W-10 with a high cellulase yield. A preservation number of the Sartorya Vuill W-10 is CGMCC No.11991. By fermenting the Sartorya Vuill W-10 disclosed by the invention, high-yield cellulose crude enzyme liquid can be obtained; cellulase filter paper enzyme activity can reach 1.26U / ml; the cellulase obtained from fermentation can be applied to preparing bioethanol, a straw residue enzymolysis rate and an ethanol yield can be effectively improved, and certain application value is achieved. The Sartorya Vuill W-10 disclosed by the invention not only has low production cost, high ethanol yield and favorability for commercial application and implementation of biological ethanol, but also provides a way for comprehensively utilizing biological waste.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation engineering, and relates to an aspergillus amylospora capable of realizing high yield of cellulase and a method for fermenting and producing cellulase by using the aspergillus amyrrhizae. Background technique [0002] The resource utilization of lignocellulosic waste such as straw can not only reduce its negative impact on the environment, but also become an effective source of renewable and sustainable energy. For second generation bioethanol production, lignocellulose has become the most viable technology option (Belal, E.B. Bioethanol production from rice straw residues. Braz JMicrobiol. 2013, 44(1):225-234). During the ethanolization process of lignocellulose, cellulase is used to hydrolyze lignocellulose, and the hydrolyzate is fermented to produce bioethanol; among them, the production of cellulase directly affects the hydrolysis of lignocellulose and the subsequent fermentati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N9/42C12P7/10C12P19/14C12P19/02C12R1/645C12R1/865
CPCC12N9/2437C12P7/10C12P19/02C12P19/14C12N1/145C12R2001/645Y02E50/10
Inventor 刘洋邱忠平孟涛龚正君王冬梅樊超李明星汤国雄
Owner SOUTHWEST JIAOTONG UNIV
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