A kind of preparation method of hydroxyethyl chitosan nanocomposite bone scaffold material

A hydroxyethyl chitosan and nano-composite technology, applied in the field of biomedical materials, can solve problems such as poor mechanical properties, and achieve low cost, simple and easy-to-operate preparation process, and good multiplication effects

Active Publication Date: 2020-11-27
XINYANG NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Bioceramics made of calcium phosphate all have poor mechanical properties and have limitations in load-bearing areas

Method used

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  • A kind of preparation method of hydroxyethyl chitosan nanocomposite bone scaffold material
  • A kind of preparation method of hydroxyethyl chitosan nanocomposite bone scaffold material
  • A kind of preparation method of hydroxyethyl chitosan nanocomposite bone scaffold material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Preparation of biphasic calcium phosphate nanopowder: 0.4M Ca(NO 3 ) 2 .4H 2 O solution and 0.2M (NH 4 ) 2 HPO 4 The solution was added into a three-necked flask according to the calcium-phosphorus molar ratio of 1.65:1, stirred and mixed evenly, and then the pH was adjusted to 11 with 25% ammonia water to obtain a white powder precipitate. After filtering, the white powder was washed with deionized water. Then dry it at 80°C, put it into a muffle furnace for roasting, first raise the temperature to 1125°C at a rate of 10°C / min, keep it for 1 hour, then drop it to room temperature with the furnace, then take it out and grind it, and finally pass it through a 200-mesh sieve to obtain a two-phase Calcium phosphate nanopowder;

[0025] (2) prepare hydroxyethyl chitosan: get 10g chitosan and join in the 50wt% sodium hydroxide solution of 16.7g and carry out alkalization, obtain chitosan solution, described alkalization is carried out in-20 ℃ refrigerator, The alka...

Embodiment 2

[0029] The preparation process is basically the same as in Example 1, except that the mass ratio of hydroxyethyl chitosan, cellulose, and biphasic calcium phosphate nanopowder in step (2) is 2:1:1.

[0030] The scanning electron micrograph of the composite bone scaffold material that embodiment 2 makes is shown in figure 1 ,in, figure 1 A is the composite bone scaffold material of embodiment 1, figure 1 B is the composite bone scaffold material of Example 2.

Embodiment 3

[0032] The preparation process is basically the same as in Example 1, except that the mass ratio of hydroxyethyl chitosan, cellulose, and biphasic calcium phosphate nanopowder in step (2) is 1:1:2.

[0033] The scanning electron micrograph of the composite bone scaffold material that embodiment 3 makes is shown in figure 1 ,in, figure 1 A is the composite bone scaffold material of embodiment 1, figure 1 B is the composite bone scaffold material of embodiment 2, figure 1 C is the composite bone scaffold material of Example 3.

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Abstract

The invention relates to a preparation method of a hydroxyethyl chitosan nano composite bone support material. The preparation method comprises the following steps: obtaining phosphorus calcium powderin a precipitating method, roasting, and obtaining hydroxyapatite / beta-tricalcium phosphate two-phase phosphorus calcium nano powder, performing the hydroxyethylation on chitosan, preparing hydroxyethyl chitosan, finally dissolving the hydroxyethyl chitosan in a 1 percent acetum solution, mixing and stirring the hydroxyethyl chitosan, gelatin and two-phase phosphorus calcium nano powder accordingto a mass ratio of (1 to 4) to 1 to (0.5 to 4), degassing, uniformly dispersing, circularly freezing and thawing the solution, and finally drying, thus obtaining the hydroxyethyl chitosan nano composite bone support material. The preparation method is simple in preparation process, easy in operation, capable of realizing the batched preparation, lower in cost, and pollution-free; and by adoptinga ternary material system, a prepared bone support has an appropriate degradation rate, the cell compatibility of the bone support is improved, the biological activity is better, and the cell can be better adhered and proliferated.

Description

technical field [0001] The invention relates to a preparation method of a hydroxyethyl chitosan nanocomposite bone scaffold material, belonging to the technical field of biomedical materials. Background technique [0002] Chitosan is obtained by deacetylation of chitin, which exists widely in nature. This natural polymer has excellent properties such as biocompatibility, blood compatibility, safety, and microbial degradability. One of the better materials. However, chitosan gel needs to be cross-linked during the preparation process, and the cross-linking agent is usually glutaraldehyde. The introduction of the cross-linking agent will reduce the cytocompatibility of the scaffold, and the mechanical properties are also poor. [0003] Bone tissue contains a large amount of phosphorus-calcium materials, such as tricalcium phosphate, hydroxyapatite, calcium carbonate, etc., so phosphorus-calcium materials are often used to prepare artificial bone scaffolds. Calcium phosphate-...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/48A61L27/46A61L27/58
CPCA61L27/46A61L27/48A61L27/58A61L2430/02C08L5/08C08L1/02
Inventor 聂磊侯孟娟杨亚男李星辰董婧
Owner XINYANG NORMAL UNIVERSITY
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