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Preparation method of nano-bone scaffold composite material

A composite material and bone scaffold technology, applied in the field of biomedical materials, can solve the problems of limitations, low activity of bone scaffolds, difficult to control mechanical properties, etc., achieve low cost, improve physicochemical properties and biological properties, and improve cell compatibility. Effect

Inactive Publication Date: 2018-09-28
XINYANG NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the conventional composite method has disadvantages such as low activity of the bone scaffold and difficulty in controlling the mechanical properties. In addition, the attachment and proliferation of cells on the bone scaffold requires the help of active substances.
The above problems limit the application of chitosan and phosphorus-calcium composite scaffolds in the field of bone tissue

Method used

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  • Preparation method of nano-bone scaffold composite material
  • Preparation method of nano-bone scaffold composite material
  • Preparation method of nano-bone scaffold composite material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A preparation method of nano-bone scaffold composite material, comprising the steps of:

[0024] (1) Preparation of biphasic calcium phosphate nanopowder: 0.4M Ca(NO 3 ) 2 4H 2 O solution and 0.2M (NH 4 ) 2 HPO 4 The solution was added into a three-necked flask according to the calcium-phosphorus molar ratio of 1.65:1, stirred and mixed evenly, and then the pH was adjusted to 11 with 25% ammonia water to obtain a white powder precipitate. After filtering, the white powder was washed with deionized water. Then dry it at 100°C, put it into a muffle furnace for roasting, first raise the temperature to 1125°C at a rate of 10°C / min, keep it for 1 hour, then lower it to room temperature with the furnace, then take it out and grind it, and finally pass it through a 200-mesh sieve to obtain a two-phase Calcium phosphate nanopowder;

[0025] (2) prepare hydroxyethyl chitosan: get 10g chitosan and join in the 50wt% sodium hydroxide solution of 16.7g and carry out alkalizati...

Embodiment 2

[0029] A preparation method of nano-bone scaffold composite material, comprising the steps of:

[0030] (1) Preparation of biphasic calcium phosphate nanopowder: 0.4M Ca(NO 3 ) 2 4H 2 O solution and 0.2M (NH 4 ) 2 HPO 4 The solution was added into a three-necked flask according to the calcium-phosphorus molar ratio of 1.60:1, stirred and mixed evenly, and then the pH was adjusted to 11 with 25% ammonia water to obtain a white powder precipitate. After filtering, the white powder was washed with deionized water. Then dry it at 100°C, put it into a muffle furnace for roasting, first raise the temperature to 1125°C at a rate of 10°C / min, keep it for 1 hour, then lower it to room temperature with the furnace, then take it out and grind it, and finally pass it through a 200-mesh sieve to obtain a two-phase Sodium Phosphate

[0031] rice powder;

[0032] (2) prepare hydroxyethyl chitosan: get 10g chitosan and join in the 50wt% sodium hydroxide solution of 16.7g and carry out ...

Embodiment 3

[0036] A preparation method of nano-bone scaffold composite material, comprising the steps of:

[0037] (1) Preparation of biphasic calcium phosphate nanopowder: 0.4M Ca(NO 3 ) 2 4H 2 O solution and 0.2M (NH 4 ) 2 HPO 4 The solution was added into a three-necked flask according to the calcium-phosphorus molar ratio of 1.55:1, stirred and mixed evenly, and then the pH was adjusted to 11 with 25% ammonia water to obtain a white powder precipitate. After filtering, the white powder was washed with deionized water. Then dry it at 100°C, put it into a muffle furnace for roasting, first raise the temperature to 1125°C at a rate of 10°C / min, keep it for 1 hour, then lower it to room temperature with the furnace, then take it out and grind it, and finally pass it through a 200-mesh sieve to obtain a two-phase Calcium phosphate nanopowder;

[0038] (2) prepare hydroxyethyl chitosan: get 10g chitosan and join in the 50wt% sodium hydroxide solution of 16.7g and carry out alkalizati...

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Abstract

The invention relates to a preparation method of a nano-bone scaffold composite material. Phosphorus calcium powder is obtained through a precipitation method firstly, and after roasting treatment, hydroxyapatite / beta-tricalcium phosphate diphasic phosphorus calcium nano powder is obtained; then chitosan is subjected to hydroxyethylation, and hydroxyethyl chitosan is prepared; and finally, the hydroxyethyl chitosan is dissolved in an acetate solution of 1%, the hydroxyethyl chitosan, gelatin and the diphasic phosphorus calcium nano powder are mixed and stirred according to the mass ratio of the hydroxyethyl chitosan to the gelatin to the diphasic phosphorus calcium nano powder being (1-6):(1-3):1, degassing is conducted for even dispersing, and the nano-bone scaffold composite material isobtained through freeze drying. A nano-bone scaffold is prepared from a novel high polymer material according to the different proportions in a mixed mode, the biological activity is improved, the cell adhesive capacity is improved, meanwhile the antibacterial property can be achieved, and the bone scaffold can be applied to the aspect of bone repairing better.

Description

technical field [0001] The invention relates to a preparation method of a nano-bone scaffold composite material, which belongs to the technical field of biomedical materials. Background technique [0002] Chitosan (chitosan) is obtained from chitin (chitin), which exists widely in nature, through deacetylation. This natural polymer has excellent properties such as biocompatibility, blood compatibility, safety, and microbial degradation. , it can provide biological activity for the scaffold, so that cells can attach to the bone scaffold, and it is a better bone scaffold material in the field of biomedicine. As a bone scaffold material, chitosan has insufficient mechanical properties and needs to be cross-linked. The cross-linking agent is usually glutaraldehyde or water-soluble carbodiimide, but the introduction of the cross-linking agent will reduce the cytocompatibility of the scaffold. [0003] Bone tissue contains a large amount of phosphorus-calcium materials, such as t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/12A61L27/20A61L27/22A61L27/54A61L27/50
CPCA61L27/12A61L27/20A61L27/222A61L27/50A61L27/54A61L2300/232A61L2300/412A61L2430/02C08L5/08
Inventor 聂磊马爽武巧运李培董婧
Owner XINYANG NORMAL UNIVERSITY
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