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Preparation method of biosorption type marine aspergillus niger mycelium pellets

A technology of aspergillus niger and biological adsorption, which is applied in the field of environmental protection, can solve the problems of easy corruption of organisms, small adsorption capacity, and slow adsorption speed, and achieve excellent microbial film-hanging performance, large adsorption capacity, and fast adsorption rate Effect

Inactive Publication Date: 2018-09-04
FOSHAN WANYANG BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The technical problem to be solved by the present invention: Aiming at the problem that fungi are used for waste water treatment, the adsorption speed is slow, the adsorption amount is not large, and organisms are easy to spoil and deteriorate, and secondary pollution will occur if they are discarded randomly, a biological adsorption marine black fungus is provided. The preparation method of aspergillus mycelia ball

Method used

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  • Preparation method of biosorption type marine aspergillus niger mycelium pellets

Examples

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example 1

[0023] Take 100g of loofah and put it into a pulverizer for crushing, pass through a 60-mesh sieve, put the sieved loofah powder into a muffle furnace, and raise the temperature at 30°C / min to 200°C in a high-purity argon atmosphere, and keep it warm React for 1 hour, then raise the temperature at 20°C / min to 1000°C, keep the temperature for 20 minutes, cool to room temperature, transfer to a ball mill, and ball mill at 200r / min for 2 hours to obtain an activated carbon substrate. Take 4g of magnesium acetate and add 100g of ethylene glycol Stir at 300r / min for 20min at 30°C, then add 10g of activated carbon substrate, continue to stir for 1h, put it into a hydrothermal reactor, react at 150°C for 2h, cool to room temperature, take out the precipitate, and use The precipitate was washed with ionized water for 3 times and then dried to obtain a surface-modified activated carbon substrate. Take 10 g of surface-modified activated carbon substrate, 10 g of glucose, 10 g of peptone,...

example 2

[0025] Take 150g of loofah and put it into a pulverizer for crushing, pass through a 60-mesh sieve, put the sieved loofah powder into a muffle furnace, and raise the temperature at 30°C / min to 250°C under a high-purity argon atmosphere, and keep it warm React for 1.5h, then heat up to 1100°C at 20°C / min, keep the temperature for 25min, cool to room temperature, transfer to a ball mill, and ball mill at 250r / min for 2.5h to obtain an activated carbon substrate. Take 4.5g of magnesium acetate and add 150g In ethylene glycol, stir at 350r / min for 25min at 35°C, then add 15g of activated carbon substrate, continue to stir for 1.5h, then put it into a hydrothermal reactor, react at 175°C for 2.5h, cool to room temperature and take it out The precipitate was washed with deionized water for 4 times and then dried to obtain a surface-modified activated carbon substrate. Take 15g of surface-modified activated carbon substrate, 11g of glucose, 11g of peptone, 11g of yeast extract, 5.5g o...

example 3

[0027]Take 200g of loofah and put it into a pulverizer for crushing, pass through a 60-mesh sieve, put the sieved loofah powder into a muffle furnace, and raise the temperature at 30°C / min to 300°C in a high-purity argon atmosphere, and keep it warm React for 2 hours, then raise the temperature at 20°C / min to 1200°C, keep the temperature for 30 minutes, cool to room temperature, transfer to a ball mill, and ball mill at 300r / min for 3 hours to obtain an activated carbon substrate. Take 5g of magnesium acetate and add 200g of ethylene glycol Stir at 400r / min for 30min at 40°C, then add 20g of activated carbon substrate, continue to stir for 2h, put it into a hydrothermal reactor, react at 200°C for 3h, cool to room temperature, take out the precipitate, and use The precipitate was washed with deionized water for 5 times and then dried to obtain the surface-modified activated carbon substrate. Take 20g of the surface-modified activated carbon substrate, 12g of glucose, 12g of pep...

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Abstract

The invention relates to a preparation method of biosorption type marine aspergillus niger mycelium pellets, belonging to the technical field of environment protection. According to the preparation method, an activated carbon substrate is prepared by virtue of the hollow porous property of loofah sponge, the specific surface area of the whole substrate material is increased by virtue of a two-section heating method, so that the substrate has excellent microorganism biofilm formation performance; nano-magnesium oxide is uniformly grown on the surface of the activated carbon substrate, so that the surface roughness is increased, the specific surface area and microorganism adhesion area of the activated carbon substrate are further increased, and the biological adhesion and adsorption properties of the activated carbon substrate are further improved; finally, marine aspergillus niger is cultured on the activated carbon substrate, and the relatively compact mycelium pellets with uniform size are formed; and the marine aspergillus niger is assisted with the activated carbon substrate for adsorbing and degrading pollutants, the adsorption speed is high, the adsorption amount is high, meanwhile, the raw material cost is low, the reserves are rich, the production process is simple, the toxic reaction caused to microorganisms is avoided, and the secondary pollution caused to a water body is avoided.

Description

technical field [0001] The invention relates to a method for preparing biologically adsorbed marine Aspergillus niger mycelia balls, belonging to the technical field of environmental protection. Background technique [0002] Mycelium ball is a kind of microbial particle formed in the process of microbial cultivation, generally only fungi or actinomycetes can form. Mycelium balls have many advantages, such as strong viability, fast sedimentation speed, easy solid-liquid separation, and can be reused. Many filamentous fungi, such as Penicillium, Aspergillus and Trichoderma, etc., have their hyphae entangled with each other to form mycelial balls in a specific liquid culture process, which can be regarded as a self-immobilization process. There have been many reports on the cultivation and utilization of mycelium balls. In industry, mycelium balls are mostly used in fermentation production, such as the production of lyric acid, enzymes and antibiotics, and have achieved id...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/685
CPCC12N1/14
Inventor 刘茂龙张桂芳李静
Owner FOSHAN WANYANG BIOLOGICAL TECH CO LTD