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Composite nano hemostatic material based on natural polysaccharide/short peptide and preparation method thereof

A hemostatic material and natural polysaccharide technology, applied in the field of nano-medical hemostatic materials, can solve the problems of limited hemostatic effect of carboxymethyl chitosan, long blood clot formation time, tens of seconds or even longer, etc. The effect of improving the happy life index, excellent hemostatic properties, and reducing the cost of synthesis

Inactive Publication Date: 2018-09-21
CHINA UNIV OF PETROLEUM (EAST CHINA)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] However, the hemostatic effect of carboxymethyl chitosan is very limited, because the blood coagulation process of carboxymethyl chitosan mainly relies on the formation of blood clots, and the formation time of blood clots is relatively long. In addition, the bleeding site usually has a relatively high pressure. Large, carboxymethyl chitosan is not easy to adhere to the wound site and form a blood clot to achieve the purpose of hemostasis
[0012] At present, there is still a significant problem in the blood coagulation materials in the prior art, that is, the blood coagulation speed generally takes tens of seconds or even longer, such as Liu Xia et al. Glucose hemostasis and its biosafety evaluation, China Tissue Engineering Research Vol. 21 No. 22] Using carboxymethyl chitosan to hemostatic the wound of the rat tail vein was about 51.6s, and the hemostasis time of the liver wound was about 40s
[0013] It is well known to those skilled in the art that blood coagulation speed is a key factor in considering hemostatic materials, however, researchers in this discipline are unable to break through this technical bottleneck

Method used

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  • Composite nano hemostatic material based on natural polysaccharide/short peptide and preparation method thereof
  • Composite nano hemostatic material based on natural polysaccharide/short peptide and preparation method thereof
  • Composite nano hemostatic material based on natural polysaccharide/short peptide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] The target polypeptide was synthesized by Fmoc microwave-assisted solid-phase synthesis of polypeptide.

[0068] Step 1: redistill the DMF and piperidine used in the experiment.

[0069] Step 2: Use the software on the peptide synthesizer to calculate the amount of each amino acid, deprotecting agent, activating agent, activating base, and capping agent in the synthesized target polypeptide, weigh the corresponding medicines, and prepare a solution. To synthesize 0.25mM Ac-IIIQGK-NH 2 As an example, the amount of amino acid, resin, deprotecting agent, activator, activating base, and capping agent required is shown in the table below:

[0070] Table 3 The dosage and preparation method of each drug

[0071]

[0072]

[0073] After preparing the required solution according to the above table, put the swollen resin into a microwave reactor, use a deprotecting agent to remove the Fmoc group on the amino group, and then the exposed amino group and the carboxyl group o...

Embodiment 2

[0077] Example 2: Determination of the secondary structure of the natural polysaccharide / short peptide composite nanohemostatic material prepared in the present invention.

[0078] Preparation of 25mM Hepes solution: Weigh 2.9789mg Hepes salt, dissolve it in 400mL ultrapure water, then adjust its pH=7.4 with NaOH solution, then add ultrapure water to a 500mL volumetric flask to constant volume. Using Hepes solution as a solvent, weigh a certain amount of polypeptide to prepare a polypeptide solution, oscillate with a rotary oscillator for 5 minutes, sonicate for 30 minutes, heat in a water bath for 2 hours, and stand for 48 hours for use. Weigh a certain amount of CMCS and dissolve it in the Hepes buffer solution, add an equal volume of the CMCS solution to the polypeptide solution, so that the final concentrations of the polypeptide and CMCS are 7.06mM and 0.44mg / mL, respectively, and pour the above solution until the optical path length is 0.1 In the quartz cell of mm, the w...

Embodiment 3

[0082] Ac-IIIQGK-NH in the present invention 2 And CMCS+Ac-IIIQGK-NH 2 The self-assembly morphology test, including atomic force microscope (AFM) and transmission electron microscope (TEM) test, the specific operation steps are as follows:

[0083] AFM test: Take a certain amount of the solution to be tested on the peeled mica sheet, absorb it for a certain period of time, then rinse it with a certain amount of ultrapure water, and then blow it with nitrogen until the mica sheet is dry. Put the obtained sample on the detection table, and use the tapping mode to scan the sample shape. The scanning angle is 0°, the scanning rate is 512×512, and the scanning rate is 1 Hz. The same sample needs to be scanned repeatedly to determine its true shape. From image 3 In (3a), (3b) and Figure 4 It can be seen from (4a) and (4b) that Ac-IIIQGK-NH 2 And CMCS+Ac-IIIQGK-NH 2 In Hepes buffer solution, it is a ribbon structure.

[0084] TEM test: use a pipette gun to draw a small amount...

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Abstract

The invention discloses a composite nano hemostatic material based on natural polysaccharide / short peptide and preparation method thereof, and belongs to the technical field of nanometer medical hemostatic material. The material is prepared by compounding natural polysaccharide and short peptide, wherein the natural polysaccharide comprises chitosan and chitosan derivative, hyaluronic acid and hyaluronic acid derivative, sodium alginate and sodium alginate derivative, and the amino acid sequence of the short peptide is X3-4QK1-2 or QX (3-4) K1-2. The composite nano hemostatic material has goodhemostatic performance, can obviously reduce bleeding time and blood loss, and has the advantages of low synthesis cost, easy synthesis and control, low biological toxicity, good biocompatibility andthe like, meets the requirements of nano hemostatic materials, can be prepared into a hemostatic medicament with clinical value, and has great significance for the development of the nano hemostaticmaterials.

Description

technical field [0001] The invention belongs to the technical field of nano medical hemostatic materials, in particular to a composite nano hemostatic material based on natural polysaccharide / short peptide and a preparation method thereof. Background technique [0002] In daily life, excessive blood loss caused by natural or artificial factors directly threatens the life safety of organisms. Therefore, it is particularly important to develop a rapid and effective hemostatic material in today's increasingly scientific development. Commonly used and representative hemostatic materials in the prior art include dry fibrin sealant dressing (Dry Fibrin Sealant Dressing, DFSD), WoundStat hemostatic agent (WoundStat, WS), QuikClot hemostatic agent (QuikClot, QC), combat gauze, HemCon Chitosan Hemostatic Agent (HemCon Chitosan Dressing, HCD) and the like. [0003] The above-mentioned hemostatic materials also have certain disadvantages when hemostasis, such as: [0004] DFSD is com...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L24/00
CPCA61L24/0031A61L24/0094A61L2400/04C08L5/08C08L5/04
Inventor 徐海郝瑞瑞陈翠霞
Owner CHINA UNIV OF PETROLEUM (EAST CHINA)
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