Florfenicol and florfenicol amine antigen, antibody and simultaneous detection of enzyme-linked immuno sorbent assay analytical method thereof

A technology of florfenicol and florfenicol, which is applied in the field of antibody and its simultaneous detection enzyme-linked immunoassay, florfenicol and florfenicol antigen, which can solve the problem of expensive equipment, the need for professionals and samples problems such as low throughput, to achieve the effect of good accuracy and high sensitivity
CN108558718AActive Publication Date: 2018-09-21SOUTH CHINA AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SOUTH CHINA AGRI UNIV
Publication Date
2018-09-21

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Abstract

The invention discloses a florfenicol and florfenicol amine antigen, antibody and a simultaneous detection enzyme-linked immuno sorbent assay analytical method thereof. The simultaneous detection enzyme-linked immuno sorbent assay analytical method utilizes florfenicol as a hapten to be coupled with a carrier protein to obtain an artificial antigen, and the artificial antigen is used as a coatingantigen; meanwhile, the hydroxyl group of the florfenicol molecule derives the succinic acid half ester to couple with arm to obtain the florfenicol hapten, and the florfenicol artificial antigen is obtained as an immunogen by coupling with the carrier protein; the detection antibody is prepared, and the enzyme-linked immuno sorbent assay analytical method is established. The IC50 of florfenicol is 37.10ng / mL, the linear detection range is 12.03-114.49ng / mL, the IC50 of florfenicol amine is 45.3ng / mL, and the linear detection range is 10.76-190.69ng / mL. The method can be used for quickly detecting the residual florfenicol and florfenicol amine in the food, and has wide application prospect.
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Description

technical field

[0001] The invention relates to the technical field of detection of florfenicol and florfenicol amine, and more specifically relates to an enzyme-linked immunoassay method for simultaneous detection of florfenicol and florfenicol amine antigens, antibodies and the same. Background technique

[0002] Florfenicol (Florfenicol, C 12 h 14 Cl 2 FNO 4 S) belongs to chloramphenicol broad-spectrum antibiotics, and is widely used in fields such as aquaculture, animal husbandry, and treatment of poultry diseases. Compared with chloramphenicol, which can cause serious harm to human health and is listed as a banned drug in many countries, thiamphenicol can cause aplastic anemia in animals, etc. Florfenicol has similar antibacterial activity to chloramphenicol and thiamphenicol And it is safer to use. However, it has been reported that florfenicol has certain immunotoxicity and embryotoxicity, and many countries have stipulated the maximum residue limit of florfenico...

Claims

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