An anthraquinone-modified graphene quantum dot aag and its preparation method and its application in the preparation of lysine fluorescence detection reagent

A graphene quantum dot, fluorescence detection technology, applied in the directions of fluorescence/phosphorescence, chemical instruments and methods, luminescent materials, etc., can solve the problems of slow development, complex synthesis methods, low compound solubility, etc., and achieves convenient operation and synthesis methods. Simple, highly selective effects

Active Publication Date: 2021-09-24
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the development of these methods is relatively slow due to the low solubility of the compounds in water or the complicated synthesis methods.

Method used

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  • An anthraquinone-modified graphene quantum dot aag and its preparation method and its application in the preparation of lysine fluorescence detection reagent
  • An anthraquinone-modified graphene quantum dot aag and its preparation method and its application in the preparation of lysine fluorescence detection reagent

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Experimental program
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Effect test

Embodiment 1

[0016] Synthesis of compound AAG

[0017] (1) Take 60 mL of 3.0 mg / mL graphene quantum dot aqueous solution in a 100 mL beaker, add dropwise 0.15 mL of N-hydroxysuccinimide and 1-(3-dimethylaminopropyl)-3 - A mixed solvent of ethylcarbodiimide hydrochloride was used as a catalyst, and it was activated by standing still for 10 min. Weigh 0.03 g of 1-aminoanthraquinone and dissolve it in 10 mL of alcohol solution, such as alcohol solution such as methanol or ethanol. Add dropwise to the above-mentioned activated graphene quantum dots, heat and ultrasonically disperse uniformly in a water bath at 40°C for 10 minutes, heat in a water bath at 55°C for 5 hours, and then stir at room temperature in the dark for 24 hours. Dialyze in 1000 mL deionized water for three days, and change the water every 3 hours to obtain anthraquinone-modified graphene quantum dots for the detection of lysine.

[0018] (2) Take 40 mL of 2.0 mg / mL graphene quantum dot aqueous solution in a 100 mL beaker, ...

Embodiment 2

[0019] Embodiment 2 (selective experiment)

[0020] In the fluorescence experiment, the compound AAG was made into a 0.02 mg / mL aqueous stock solution, and the biomolecules were selected from lysine, alanine, arginine, glycine, glucose, glucosamine, lysine, maltose, lactose, sucrose, fructose and other substances , all experimental solutions were freshly prepared and tested immediately. Emitting at 432 nm, the biomolecules were tested separately. In the experiment, 2.5 mL of the stock solution was taken, and 1M biomolecules solution was added respectively. Test its fluorescence spectrum.

Embodiment 3

[0021] Example 3 Coexistence of Interfering Substances to Detect Lysine Experiment

[0022] In the fluorescence experiment, the compound AAG was prepared as a 0.025 mg / mL aqueous solution. Lysine was prepared as a 1M standard stock solution. Glycine, arginine, glucose, glucosamine, maltose, lactose, sucrose, fructose and other substances are selected as the biomolecules of the interfering substance. All experimental solutions were freshly prepared and tested immediately. In the interfering substance experiment, first add 5 times the interfering substance to the 0.025 mg / mL AAG aqueous solution to measure its fluorescence, then add 1M lysine to measure its fluorescence change. Fluorescence changes were detected at 432 nm.

[0023] Mechanism of the invention: due to the hydrogen bond interaction between lysine and the compound, the electron energy in the molecule changes and the fluorescence intensity changes, so as to achieve the purpose of detecting lysine. Glucose, glucos...

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Abstract

An anthraquinone-modified graphene quantum dot AAG and its preparation method and its application in the preparation of a lysine fluorescence detection reagent, the anthraquinone is introduced into the water-soluble nano-quantum dot, specifically for example, 1-aminoanthraquinone Introduced into graphene quantum dots, graphene quantum dots with luminescent properties and good water solubility are formed by connecting anthraquinone through amide bonds to form graphene quantum dots with dual luminescent properties. The synthesis method is simple, the conditions are mild, and the product is easy Obtained, the compound is used for the detection of lysine of the present invention to obtain good results, and is not affected by other conventional coexisting biomolecules, such as alanine, glycine, arginine, methionine, glucose, glucosamine and other substances, and has high selectivity. The fluorescence spectrophotometer is easy to operate, and the sample fluorescence signal is obvious.

Description

technical field [0001] The invention relates to the field of molecular detection techniques for recognition, binding and optical detection of lysine, in particular to an anthraquinone-modified graphene quantum dot AAG, a preparation method thereof, and an application in the preparation of a lysine fluorescence detection reagent. Background technique [0002] Lysine is an essential amino acid for humans and animals, involved in the Krebs–Henseleit cycle and the synthesis of polyamines (Yoshida, H., Nakano, Y., Koiso, K., et al. Anal. Sci., 2001, 17, 107. Wellner, D., Meister, A. Annu. Rev. Biochem., 1981, 50, 911.). Lysine dietary imbalance, like certain inborn errors of metabolism, can cause cystinuria or hyperlysinemia (Felig, P. Annu. Rev. Biochem., 1975, 44, 933. Hirayama, C. , Suyama, K., Horie, Y., et al. Biochem. Med. Metab. Biol., 1987, 38, 127. ). There are many methods for detecting lysine in the world at present, including electrochemical analysis, electrophoresi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D303/38C09K11/06C09K11/65B82Y20/00B82Y40/00G01N21/64
CPCB82Y20/00B82Y40/00C07D303/38C09K11/06C09K11/65G01N21/64G01N21/6428G01N2021/6417G01N2021/6439
Inventor 程如梅丁阳张立树邹睿韬
Owner WENZHOU MEDICAL UNIV
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