Preparation method and application of indocyanine green polymer nano particles

A technology of indocyanine green and nano-particles, which is applied in the field of medicine, can solve the problems of optical property change and short half-life, and achieve the effects of short preparation cycle, low organic residue and good stability

Active Publication Date: 2018-11-23
HUAQIAO UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the rapid aggregation of indocyanine green in aqueous solution, the short half-life in the body (about 2-4min), and the shortcoming of easily combining with hemoglobin to cause changes in its optical properties limit its application.

Method used

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  • Preparation method and application of indocyanine green polymer nano particles
  • Preparation method and application of indocyanine green polymer nano particles
  • Preparation method and application of indocyanine green polymer nano particles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Weigh 20 mg of indocyanine green in 10 ml of ethanol solution, stir and dissolve, add 10 ml of dichloromethane dropwise to the prepared solution, stir and mix evenly, then place the mixture in a kettle connected to a solvent pump, and dissolve the mixture at 0.5 ml / min The liquid flow rate is pumped into the supercritical granulation autoclave, and the pressure is 10MPa, the temperature is 35°C, and the flow rate of carbon dioxide is 40g / min; particles. Weigh 1mg of polylysine and dissolve it in 0.5mol / L sodium chloride solution to obtain a mixed solution, weigh 4mg of indocyanine green nanoparticles into the mixed solution, ultrasonically disperse it, and stir at room temperature for 4h , centrifuged at 8000r / min for 10min, added ultrapure water and ultrasonically repeated the centrifugation operation twice, and obtained indocyanine green-polylysine nanoparticles (ICG-PLL) after freeze-drying. Nanoparticles were ultrasonically dispersed in ultrapure water, shaken with...

Embodiment 2

[0031] This example is basically the same as Example 1, except that the solvent used is hexafluoroisopropanol / acetone, and the solvent ratio is still 5:5, and finally the indocyanine green-polylysine nano particles;

Embodiment 3

[0033] Get each 200 μ l of indocyanine green-polylysine nanoparticles and indocyanine green solution prepared in Example 1 (C ICG =0.1mg / ml) The solvent is ultrapure water, through near-infrared 808nm, 1.5W / cm 2 Near-infrared irradiation was performed for 5 minutes, and the temperature change was recorded by a near-infrared temperature imager.

[0034] image 3 It is the photothermal curve of indocyanine green nanoparticles and indocyanine green-polylysine nanoparticles. Under the same conditions, indocyanine green-polylysine nanoparticles can cause the solution to dissolve under 808nm laser irradiation. significantly increased, and the effect is better than the same concentration of indocyanine green aqueous solution, indicating that the indocyanine green-polylysine nanoparticles prepared in the present invention have excellent light-to-heat conversion performance.

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Abstract

The invention discloses a preparation method and application of indocyanine green polymer nano particles. The preparation method comprises the following steps: (1) dissolving indocyanine green in a first organic solvent, dropwise adding a second organic solvent, and obtaining a mixture; (2) pumping the mixture into a supercritical granulation autoclave, and after the mixture is pumped, continuously flushing by using supercritical carbon dioxide, thus obtaining the indocyanine green nano particles; and (3) ultrasonically dispersing the indocyanine green nano particles in a sodium chloride solution comprising polycations, stirring, then centrifuging, washing obtained precipitates by using ultrapure water, and then freeze drying, thus obtaining the indocyanine green polymer nano particles. The indocyanine green polymer nano particles prepared in the invention are good in stability, an optical absorption wavelength range is at a near-infrared waveband, the high penetration performance of the near-infrared rays for biological tissues can be well utilized, and the indocyanine green polymer nano particles can be better used for treating tumors.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a preparation method and application of indocyanine green polymer nanoparticles. Background technique [0002] Traditional surgical treatment, chemotherapy, radiotherapy and other cancer treatment methods all have low curative effect and high toxicity and side effects. As a non-invasive cancer treatment method, photothermal therapy uses photothermal preparations to convert laser light energy into heat energy to achieve the purpose of locally killing tumor cells, which has certain advantages in cancer treatment. Indocyanine Green (ICG) is a tricarbonate dye with a characteristic absorption peak in the near infrared. It has strong fluorescence penetration and is currently the only near-infrared fluorescent reagent approved by the US Food and Drug Administration. The higher photothermal conversion efficiency of green makes it have better potential application value in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K49/00A61K9/19A61K47/34A61P35/00
CPCA61K9/19A61K41/0052A61K47/34A61K49/0034A61K49/0054A61P35/00
Inventor 陈爱政王士斌徐沛瑶潘玉晶
Owner HUAQIAO UNIVERSITY
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