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Probiotics microcapsule freeze-dried powder and preparation method thereof

A technology of probiotics and microcapsules, which is applied in the field of probiotic microcapsule freeze-dried powder and the preparation of probiotic microcapsule freeze-dried powder, which can solve the problems of large particle size, affecting food taste and quality, and poor dispersibility.

Active Publication Date: 2018-11-30
INNER MONGOLIA AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, since CaCl 2 As a soluble calcium salt, the system is full of Ca 2+ , when the temperature rises suddenly, Ca 2+ Not only will it cross-link inside the W / O emulsion, but it will also cross-link between the emulsions, resulting in a greater degree of adhesion of the formed microcapsules, poor dispersion, and large particle size, which will affect the taste and quality of food.

Method used

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  • Probiotics microcapsule freeze-dried powder and preparation method thereof
  • Probiotics microcapsule freeze-dried powder and preparation method thereof
  • Probiotics microcapsule freeze-dried powder and preparation method thereof

Examples

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Effect test

Embodiment 1

[0131] Embodiment 1: Preparation of probiotic microcapsules of the present invention and freeze-dried powder thereof

[0132] The implementation steps of this embodiment are as follows:

[0133] A. Strain activation

[0134] The probiotic Lactobacillus plantarum LIP-1 preserved by the Lactic Acid Bacteria Resource Bank of Inner Mongolia Agricultural University was inoculated in the MRS liquid medium according to the inoculation amount of 1% based on the MRS liquid medium, and cultivated in the incubator at a temperature of 37°C 21h, activated and cultured for three generations in the same way, centrifuged, and collected the sludge; according to the weight ratio of the sludge and saline 1:10, the sludge was washed twice with sterilized saline, and then mixed with sterile water The weight ratio is 1:7, resuspend the washed bacteria slime in sterile water to obtain the concentrated bacterial solution, shake and mix well, and then use the plate counting method to accurately count...

Embodiment 2

[0142] Embodiment 2: Preparation of probiotic microcapsules of the present invention and freeze-dried powder thereof

[0143] The implementation steps of this embodiment are as follows:

[0144] A. Strain activation

[0145] The probiotic Lactobacillus casei ZHANG preserved by the Lactic Acid Bacteria Resource Bank of Inner Mongolia Agricultural University was inoculated in the MRS liquid medium according to the inoculation amount of 1% based on the MRS liquid medium, and cultivated in the incubator at a temperature of 37°C for 16 hours. Activate and cultivate three generations in the same way, centrifuge and collect the sludge; according to the weight ratio of the sludge and saline of 1:12, the sludge is washed with sterilized saline for 3 times, and then the sludge is washed according to the weight of the sludge and sterile water. Ratio 1:9, resuspend the washed bacteria slime in sterile water to obtain a concentrated bacterial solution, shake and mix well, then use the pla...

Embodiment 3

[0153] Embodiment 3: Preparation of probiotic microcapsules of the present invention and freeze-dried powder thereof

[0154] The implementation steps of this embodiment are as follows:

[0155] A. Strain activation

[0156] The probiotic Bifidobacterium V9 preserved by the Lactic Acid Bacteria Resource Bank of Inner Mongolia Agricultural University was inoculated in the MRS liquid medium according to the inoculation amount of 1% based on the MRS liquid medium, and cultivated in an incubator at a temperature of 37°C for 18 hours. Activate and cultivate three generations in the same way, centrifuge and collect the sludge; according to the weight ratio of the sludge and saline 1:14, wash the sludge twice with sterilized saline, and then wash according to the weight of the sludge and sterile water Ratio 1:6, resuspend the washed bacteria slime in sterile water to obtain concentrated bacterial liquid, oscillate and mix well, then use plate counting method to accurately count the ...

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Abstract

The invention relates to a probiotics microcapsule and a preparation method of probiotics microcapsule freeze-dried powder. The preparation method comprises the following steps: activating a bacterialstrain, preparing a fermented skimmed milk bacterial solution, preparing a microcapsule, freeze drying and the like. The microcapsule particles prepared in the invention are good in morphological characteristics and compact in surface, the particle size is 70 to 130 micrometers, the coating rate is greater than 85 percent, the gastric acid resistance is good, and the intestinal juice release performance, the freeze drying resistance and the storage stability are good, so that the probiotics can be protected against the adverse environment, the health effect of the probiotics in the human bodycan be improved, and the defects of the traditional embedding method are solved. The technology for preparing the probiotics microcapsule can be widely applied to the technical fields such as food, biological technology and the like.

Description

【Technical field】 [0001] The invention belongs to the field of food biotechnology. More specifically, the present invention relates to a method for preparing probiotic microcapsule freeze-dried powder by using an aqueous phase separation method, and the present invention also relates to said probiotic microcapsule freeze-dried powder. 【Background technique】 [0002] The World Health Organization (WHO) and the Food and Agriculture Organization (FAO) jointly define probiotics as "live microorganisms that, when ingested in sufficient quantities, confer certain health benefits on the body". Probiotics have a variety of physiological functions, including improving human intestinal function, inhibiting the growth of harmful bacteria, reducing intestinal diseases, alleviating lactose intolerance, promoting the absorption of sugar, protein, calcium, magnesium and other nutrients, lowering cholesterol, regulating Immune system, anti-tumor, cancer prevention, etc. In order for probi...

Claims

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Application Information

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IPC IPC(8): A23L33/135A23P10/30
CPCA23L33/135A23P10/30A23V2002/00A23V2200/10A23V2200/3204A23V2250/1578A23V2250/54246A23V2300/20A23V2400/169A23V2400/125A23V2400/51A23V2300/10
Inventor 王俊国霍麒文麻丽丽包秋华丹彤张和平
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY
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