Analysis and detection method for neomycin sulfate

A neomycin sulfate and detection method technology, applied in the direction of analyzing materials, material analysis through optical means, measuring devices, etc., can solve the problems of inability to meet trace detection requirements, high detection limit, etc., achieve rapid detection, simple method, highly reliable effects

Active Publication Date: 2018-12-11
JIMEI UNIV
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Problems solved by technology

These methods are mainly analyzed and detected through the color reaction of the solution, and the general detection limit is high, which cannot meet the requirements of trace detection.
[0005] Although the literature has reported that nanoparticles can be aggregated by adding a small amount of specific substances, so far, there has been no report on the use of this phenomenon for the analysis and detection of antibiotics.

Method used

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  • Analysis and detection method for neomycin sulfate
  • Analysis and detection method for neomycin sulfate
  • Analysis and detection method for neomycin sulfate

Examples

Experimental program
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Effect test

Embodiment 1

[0035] 1) Synthesis of Au@PVP nanoparticles:

[0036] Au nanoparticles (AuNPs) were prepared by reducing chloroauric acid with sodium citrate. Under boiling conditions, quickly add 1.4mL 1% trisodium citrate solution to 200mL chloroauric acid solution with a mass concentration of 0.01%. After the solution turns wine red, continue the reaction for 30min, and cool naturally to room temperature to prepare Au nanoparticle sol with a particle size of about 55 nm (see figure 1 ).

[0037] Preparation of Au@PVP core-shell nanoparticles by chemical reduction method. Take 20ml of AuNPs sol in a clean flask and add 100μL of 1% PVP solution. Au@PVP core-shell nanoparticles with a PVP shell thickness of about 3.5nm can be prepared by stirring at 1000r / min for 25min at room temperature. The particle size, shape and shell thickness of the core-shell nanoparticles were characterized by transmission electron microscopy. (see figure 1 and figure 2 ).

[0038] 2) After combining the Au...

Embodiment 2

[0043] 1) Synthesis of Au@PVP nanoparticles:

[0044] Au nanoparticles (AuNPs) were prepared by reducing chloroauric acid with sodium citrate. Under boiling conditions, quickly add 1.4mL 1% trisodium citrate solution to 200mL chloroauric acid solution with a mass concentration of 0.01%. After the solution turns wine red, continue the reaction for 30min, and cool naturally to room temperature to prepare Au nanoparticle sol with a particle size of about 55 nm (see figure 1 ).

[0045] Preparation of Au@PVP core-shell nanoparticles by chemical reduction method. Take 20ml of AuNPs sol in a clean flask, add 100μL of 1% PVP solution, and stir at 1000r / min for 25min at room temperature to prepare Au@PVP core-shell nanoparticles with a PVP shell thickness of about 3.5nm. The particle size, shape and shell thickness of the core-shell nanoparticles were characterized by transmission electron microscopy (see figure 1 ).

[0046] 2) The optimal ratio of Au@PVP core-shell nanoparticle...

Embodiment 3

[0050] 1) Synthesis of Au@PVP nanoparticles:

[0051] Au nanoparticles (AuNPs) were prepared by reducing chloroauric acid with sodium citrate. Under boiling conditions, quickly add 1.4mL 1% trisodium citrate solution to 200mL chloroauric acid solution with a mass concentration of 0.01%. After the solution turns wine red, continue the reaction for 30min, and cool naturally to room temperature to prepare Au nanoparticle sol with a particle size of about 55 nm (see figure 1 ).

[0052] Preparation of Au@PVP core-shell nanoparticles by chemical reduction method. Take 20ml of AuNPs sol in a clean flask, add 100μL of 1% PVP solution, and stir at 1000r / min for 20min at room temperature at 25°C to prepare Au@PVP core-shell nanoparticles with a PVP shell thickness of about 3.5nm. The particle size, shape and shell thickness of the core-shell nanoparticles were characterized by transmission electron microscopy (see figure 1 ).

[0053] 2) Quantitative analysis and detection of neom...

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Abstract

An analysis and detection method for neomycin sulfate relates to neomycin sulfate. The analysis and detection method for neomycin sulfate comprises the steps of synthesizing a gold nano-particle; preparing an Au@PVP core-shell nano-particle sol; weighing the Au@PVP core-shell nano-particle sol, and measuring a surface plasma resonance absorption spectrum of the Au@PVP core-shell nano-particle sol;and performing quantitative analysis and detection on neomycin sulfate. A hydrogen bond can be formed by using an amino group of neomycin sulfate and oxygen of pyrrolidone in PVP (Polyvinyl Pyrrolidone) on an Au@PVP core-shell nano-particle to induce agglomeration of the Au@PVP core-shell nano-particle, thereby causing reduction of the surface plasma resonance absorption at 542 nm and implementing the quantitative analysis and detection on neomycin sulfate. The method is simple in a sample pretreatment process, expensive instrument and equipment and higher operation technical requirements arenot needed and the requirement of rapid analysis and detection can be satisfied. The phenomenon that the nano-particle is hypersensitive to substances and thus agglomerates instantly is utilized; theanalysis and detection method is simple, rapid, visible and highly sensitive.

Description

technical field [0001] The present invention relates to neomycin sulfate, in particular to the preparation of Au@PVP core-shell nanoparticles by hydrothermal synthesis, using neomycin sulfate to induce the aggregation reaction of core-shell nanoparticles, and reducing the surface plasma of Au@PVP core-shell nanoparticles Based on the volume resonance absorption, neomycin sulfate was analyzed and detected according to the degree of reduction. Background technique [0002] Neomycin sulfate (NEO) is an aminoglycoside antibiotic formed by linking aminosugar and aminocyclic alcohol through an oxygen bridge. These antibiotics are commonly used as veterinary drugs to induce bacterial synthesis of wrong proteins and to inhibit the release of synthesized proteins, leading to bacterial death. It is often used to treat Gram-negative bacteria, such as Escherichia coli, Salmonella, Klebsiella, and Aerobacter aerogenes, and to treat intestinal diseases caused by Aeromonas and Edwardsiell...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/552
CPCG01N21/553
Inventor 张芹贺路影苏文金
Owner JIMEI UNIV
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