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A strain of Alcaligenes faecalis bc13 and its application in the control of bacterial diseases of crops

A technology of Alcaligenes faecalis and bacteria, which is applied to Alcaligenes faecalis BC13 and its application in the prevention and control of bacterial diseases of crops. It can solve problems such as toxicity, water resource pollution, and bacterial resistance, and achieve good antibacterial Effect, good control effect, good inhibitory effect

Active Publication Date: 2020-12-11
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, chemical agents have certain toxicity to humans and animals, and can also cause problems such as bacterial resistance and water pollution.

Method used

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  • A strain of Alcaligenes faecalis bc13 and its application in the control of bacterial diseases of crops
  • A strain of Alcaligenes faecalis bc13 and its application in the control of bacterial diseases of crops
  • A strain of Alcaligenes faecalis bc13 and its application in the control of bacterial diseases of crops

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Isolation and screening of Pseudomonas BC13

[0033] 1. Sample collection: In the rhizosphere soil of cabbage in Dalingxia Village, Yashan Village Committee, Quzhang Township, Hepu, Guangxi, soil samples below 5 cm of the soil surface were excavated and stored in bags, and brought back to the laboratory for strain isolation.

[0034] 2. Isolation of bacterial strains: Soil samples were isolated by dilution coating plate method. Specifically, after the collected soil samples were thoroughly mixed by hand, 4.0 g was weighed, soaked in 20 mL of sterile water and shaken, and then cultured on a shaker at 28 °C and 180 rpm for 20 min for gradient dilution. Take the dilution factor as 10 5 ~10 9 100 µL of the diluted solution was spread on the LB plate, air-dried at room temperature, sealed, and incubated upside down in a 28°C incubator for 24 h. Pick a single colony grown on the LB plate, label it, place it in a 2 mL centrifuge tube containing 500 µL liquid LB, s...

Embodiment 2

[0036] Example 2 Identification of biocontrol bacteria BC13

[0037] 1. Morphological and physiological and biochemical identification

[0038] Strain BC13 is a Gram-negative bacterium; BC13 colonies on LB medium are round, milky white, with smooth and tidy edges, concave table-shaped, opaque, produce catalase, do not produce amylase, do not hydrolyze gelatin, and cannot utilize half Lactose, mannitol, fructose, trehalose, glucose and sucrose are used as carbon sources to produce acid, and different organic acids and amino acids can be used as carbon sources.

[0039] 2. Molecular identification

[0040] In order to clarify the taxonomic status of the strain BC13 obtained in Example 1, the 16S rDNA sequence was used for phylogenetic analysis (Phylogenetic analysis) combined with physiological and biochemical assays to identify the BC13 strain.

[0041] 16S rDNA sequence analysis is a molecular biological identification method for bacterial identification. The present inventi...

Embodiment 3

[0043] Embodiment 3 Determination of antibacterial spectrum of biocontrol bacteria BC13

[0044] 1. In order to better study the biocontrol potential of the bacterial strain BC13 obtained in Example 1, the bacteriostatic spectrum of the bacterium was determined. The specific operation is as follows:

[0045] (1) To explore whether BC13 has the ability to inhibit the growth of other pathogenic bacteria. Use the method identical with embodiment 1 bacterial strain screening, change pathogenic bacterium MS2 into the equal amount of rice base rot pathogen EC1, banana bacterial soft rot MS3, bacterial wilt EP1 (the first layer 15 mL of LB was replaced by 15 mL of TTC), bacterial blight Xoo, oryza soft rot Pantoea ananatis SC7, peach rot pathogen P. ananatis PP1, the rest of the steps remain unchanged.

[0046] (2) To explore whether BC13 has the ability to inhibit the growth of pathogenic fungi. The fungi tested included Capsicum anthracnose, Colloidal anthracnose, Banana wil...

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Abstract

The invention discloses a strain of Alcaligenes faecalis ( Alcaligenes faecalis ) BC13 and its application in the control of crop bacterial diseases. The Alcaligenes faecalis BC13 strain was deposited in the Guangdong Microbial Culture Collection Center on July 10, 2018, with the preservation number GDMCC No: 60412. The bacterial strain BC13 of the present invention has a good inhibitory effect on crop bacterial soft rot, bacterial wilt, rice bacterial blight and Pantoea, can significantly reduce the occurrence of crop bacterial soft rot, and in pot experiments The effect is good, indicating that the BC13 strain can be used to develop biopesticides against bacterial diseases of crops. At the same time, the strain also has a good antibacterial effect on some plant pathogenic fungi, such as rice sheath blight and litchi downy mildew.

Description

technical field [0001] The invention relates to the technical field of biological control, in particular to a strain of Alcaligenes faecalis BC13 and its application in preventing and treating bacterial diseases of crops. Background technique [0002] Bacterial diseases are plant diseases caused by pathogenic bacteria infection, such as soft rot, bacterial wilt, etc., which can cause serious economic losses. [0003] For example, D. cornella ( Dickeya zeae ), in addition to infecting monocotyledonous plants, it can also infect dicotyledonous plants at the same time, and its host range includes rice, corn, sugarcane, banana, brachiaria, iris, shegan, daylily, pennisetum, pineapple, potato, tobacco , Chinese cabbage, carrots, peanuts, impatiens, chrysanthemums and other plants. The reported serious plant diseases caused by D. maize include bacterial root rot of rice and bacterial soft rot of bananas. The diseases caused by them have latent infection, resulting in a large num...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/20A01P1/00A01P3/00C12R1/05
CPCA01N63/00A01N63/10C12N1/205C12R2001/05
Inventor 周佳暖陈霞李洁玲胡明薛洋张炼辉
Owner SOUTH CHINA AGRI UNIV
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