Microorganism-supported platinum-palladium alloy nano catalyst and preparation method thereof
A nano-catalyst, platinum-palladium alloy technology, applied in the field of precious metal nano-materials, to achieve the effects of high catalytic activity, small size, and enhanced absorption
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Embodiment 1
[0044] A kind of microorganism-loaded platinum-palladium alloy nanocatalyst of the present invention, mass concentration ratio (bacteria: platinum: palladium) is 30:1:1, prepares the platinum-palladium alloy nanocatalyst that average size is 13nm and uniform dispersion, Figure 1-3 The transmission electron microscope picture, the scanning electron microscope picture and the X-ray diffraction picture of the nano-catalyst are respectively.
[0045] The preparation method of the immobilized microbial adsorbent comprises the following steps:
[0046] (1) Inoculate the bacteria liquid of Shewanella oneidensis MR-1 into 100mL of sterilized LB medium under sterile conditions, expand the culture at 30°C and 170r / min for 24h, and then inoculate at 10000rpm Centrifuge for 10 minutes to collect the wet cells after activation and culture;
[0047] (2) Prepare a mixed solution with a platinum and palladium metal ion concentration of 100 mg / L (the mass ratio of platinum and palladium meta...
Embodiment 2
[0060] A kind of microorganism-loaded platinum-palladium alloy nanocatalyst of the present invention, mass concentration ratio (bacterium: platinum: palladium) is 50:1:1, prepares the platinum-palladium alloy nanocatalyst that average size is 10nm and uniform dispersion, Figure 6-7 They are the transmission electron microscope picture and the scanning electron microscope picture of the nano-catalyst, respectively.
[0061] The preparation method of the immobilized microbial adsorbent comprises the following steps:
[0062] (1) Inoculate the bacteria liquid of Shewanella oneidensis MR-1 into 100mL of sterilized LB medium under sterile conditions, expand the culture at 30°C and 170r / min for 24h, and then inoculate at 10000rpm Centrifuge for 10 minutes to collect the wet cells after activation and culture;
[0063] (2) Prepare a mixed solution with a platinum and palladium metal ion concentration of 100 mg / L (the mass ratio of platinum and palladium metal ions is 1:1), adjust t...
Embodiment 3
[0073] A kind of microorganism-loaded platinum-palladium alloy nanocatalyst of the present invention, mass concentration ratio (bacteria: platinum: palladium) is 30:1:1, prepares the platinum-palladium alloy nanocatalyst that average size is 21nm and uniform dispersion, Figure 9-10 They are the transmission electron microscope picture and the scanning electron microscope picture of the nano-catalyst, respectively.
[0074] The preparation method of the immobilized microbial adsorbent comprises the following steps:
[0075] (1) Inoculate the bacteria liquid of Shewanella oneidensis MR-1 into 100mL of sterilized LB medium under sterile conditions, expand the culture at 30°C and 170r / min for 24h, and then inoculate at 10000rpm Centrifuge for 10 minutes to collect the wet cells after activation and culture;
[0076] (2) Prepare a mixed solution with a platinum and palladium metal ion concentration of 100 mg / L (the mass ratio of platinum and palladium metal ions is 1:1), adjust t...
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