Time resolution fluorescent immunochromatographic test strip for detecting metalaxyl, and preparation method and application thereof

A technology of time-resolved fluorescence and immunochromatography test strips, which is applied in the fields of analytical materials, biological testing, fluorescence/phosphorescence, etc., to achieve the effects of reducing costs, increasing reaction sensitivity, and simple operation

Active Publication Date: 2018-12-21
ZHENGZHOU TOBACCO RES INST OF CNTC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no time-resolved fluorescent immunochromatographic test strip for detecting metalaxyl in tobacco products, and the present invention fills this gap

Method used

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  • Time resolution fluorescent immunochromatographic test strip for detecting metalaxyl, and preparation method and application thereof
  • Time resolution fluorescent immunochromatographic test strip for detecting metalaxyl, and preparation method and application thereof
  • Time resolution fluorescent immunochromatographic test strip for detecting metalaxyl, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Composition of Time-Resolved Fluorescent Immunochromatographic Test Strips for Detecting Metalaxyl

[0042] 1. Test strips

[0043] see figure 1 : The test strip is composed of a bottom plate, a sample absorbent pad, a conjugate release pad, a nitrocellulose membrane and an absorbent pad;

[0044] The sample absorbent pad 1, the conjugate release pad 2, the nitrocellulose membrane 3 and the water absorbent pad 4 are overlapped and pasted on the bottom plate 7 in sequence, and 1 / 3 of the conjugate release pad is covered by the sample absorbent pad from the starting end , the end of the conjugate release pad is connected to the beginning of the nitrocellulose membrane, the end of the nitrocellulose membrane is connected to the beginning of the absorbent pad, the beginning of the sample absorbent pad is aligned with the beginning of the PVC bottom plate, and the end of the absorbent pad is connected to the end of the PVC bottom plate alignment;

[0045] The ni...

Embodiment 2

[0047] Example 2 Preparation of Time-Resolved Fluorescent Immunochromatographic Test Strips for Detecting Metalaxyl

[0048] The preparation method of the time-resolved fluorescent immunochromatographic test strip for detecting metalaxyl mainly comprises the following steps:

[0049] 1) Preparation of the conjugate release pad: label the metalaxyl monoclonal antibody with commercially available fluorescent microspheres, and dilute it with a specific buffer system, soak the conjugate release pad in the dilution buffer, and vacuum freeze-dry Post-preparation;

[0050] 2) Preparation of nitrocellulose membrane: Spray the metalaxyl hapten-carrier protein conjugate on the detection area of ​​the nitrocellulose membrane to make the detection area; spray goat anti-mouse anti-antibody onto the nitrocellulose membrane The scope of the quality control area above is made into the quality control area;

[0051] 3) Assembly and cutting: Paste the sample absorption pad, the conjugate rele...

Embodiment 3

[0103] Example 3 Application of time-resolved fluorescent immunochromatographic test strips for detection of metalaxyl

[0104] 1. Tobacco sample pretreatment

[0105] Weigh 1.0±0.05 g crushed tobacco sample into a polystyrene centrifuge tube; add 10 mL of 50% methanol aqueous solution, and use a homogenizer to fully break it up to obtain the sample liquid; filter it with a filter membrane, and pipette 100 μL The sample solution was mixed with 900 μL deionized water and then tested.

[0106] 2. Test with test strips

[0107] Draw 100 μL of the sample solution to be tested and drop it vertically into the sample hole of the test paper card. When the liquid flows, start timing and react for 10 minutes; insert the test paper card into the carrier of the KFT-100A fluorescence detector, and select the sample to be tested through the touch screen. item, press the "Start Test" button, the fluorescence detector will automatically scan the test paper card, and read or print the test r...

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Abstract

The invention discloses a time resolution fluorescent immunochromatographic test strip for detecting metalaxyl, and a preparation method and application thereof. The time resolution fluorescent immunochromatographic test strip comprises a bottom plate and further comprises a sample absorbent pad, a combination releasing pad, a nitrocellulose membrane and a water absorbent pad which are lapped andpasted on the bottom plate in sequence. A metalaxyl monoclonal antibody labelled by fluorescent microspheres is embedded into the combination releasing pad, and a detecting area and a quality controlarea are fixed to the nitrocellulose membrane. A metalaxyl hapten-carrier protein conjugate is sprayed in the detecting area, an anti-sheep and anti-mouse antibody is sprayed in the quality control area, and a metalaxyl hapten is obtained in the modes that N-(2,6-dimethyl phenyl) alanine methyl ester reacts with (4-nitrophenoxy) acetylchloride to generate nitrobenzene metalaxyl, and then the nitrobenzene metalaxyl is subjected to zinc powder reduction. The time resolution fluorescent immunochromatographic test strip and a detecting method provided by the invention have the advantages that operation is easy, sensitivity is high, the detecting speed is high, and the cost is low, and the metalaxyl in large-batch samples can be detected quickly and monitored on site.

Description

technical field [0001] The invention belongs to the field of pesticide residue detection, and in particular relates to a time-resolved fluorescent immunochromatographic test strip for detecting metalaxyl in tobacco and tobacco products, a preparation method and application thereof. Background technique [0002] Metalaxyl is a strong systemic substituted benzamide fungicide, chemical name N-(2-methoxyacetyl)-N-(dimethylphenyl)-racemic-aminobenzoic acid Formic acid is an amide fungicide with high efficiency and low toxicity. It is mainly used for various downy mildew, late blight, early blight, damping-off, blight, fruit rot, etc. caused by oomycetes, algae, and fungi. . It mainly inhibits the synthesis of protein in the mycelium of pathogenic bacteria, making them lack of nutrients, unable to grow normally and die. It has strong systemic absorption and penetrating power, and it can conduct bidirectionally up and down in the plant within 30 minutes after application. It has ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/58G01N33/533G01N21/64
CPCG01N21/6408G01N33/533G01N33/558G01N33/582G01N33/585
Inventor 陈黎范子彦刘惠民唐纲岭秦亚琼崔华鹏樊美娟赵乐蔡君兰王冰
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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