Lactobacillus helveticus and application thereof in bee breeding process

A Lactobacillus and bee technology, applied in the biological field, can solve the problems of intestinal flora microecological imbalance, enhance the resistance of intestinal microorganisms, bee death, etc., to improve the flora microecological structure and enhance the intestinal immunity of bees. strength, and the effect of improving survival

Active Publication Date: 2019-01-11
YUNNAN AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, during the breeding process, bees are susceptible to infectious diseases that are threatened by various external conditions, resulting in the death of a large number of bees and affecting the production capacity of the bee colony
In order to control bee infectious diseases, antibiotics are still mainly used for prevention and treatment, and there are phenomena

Method used

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  • Lactobacillus helveticus and application thereof in bee breeding process
  • Lactobacillus helveticus and application thereof in bee breeding process
  • Lactobacillus helveticus and application thereof in bee breeding process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Screening and Identification of Lactobacillus helveticus KM7

[0026] (1) Screening of Lactobacillus helveticus KM7

[0027] (1) Collection of samples

[0028] Put healthy adult worker bees of Apis mellifera randomly captured from the beekeeping farm of the Department of Apiology, College of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan Province, into a 50mL sterilized centrifuge tube, insert carbon dioxide until the bees are comatose, and place them on the ultra-clean workbench. The bees were internally fixed, and the intestines of the bees were taken out with tweezers and transferred into a 1.5mL sterilized centrifuge tube, and stored in a 4°C refrigerator for later use. The equipment used in the experiment was sterilized with ultraviolet light and 75% ethanol.

[0029] (2) Screening of lactic acid bacteria

[0030] Under sterile conditions, the gut samples of healthy adult worker bees of Apis mellifera were taken, added to 10 mL of ...

Embodiment 2

[0039] Probiotic Properties of Lactobacillus helveticus KM7

[0040] (1) Antibacterial properties of Lactobacillus helveticus KM7

[0041] The inhibitory effect of Lactobacillus helveticus KM7 on the growth and reproduction of main intestinal pathogenic bacteria was studied by agar punching diffusion method. Lactobacillus helveticus KM7 was inoculated in MRS broth medium, anaerobically cultured at 37°C for 24 hours, and the number of bacteria was adjusted to 10 with MRS broth medium. 8 CFU / mL; 4°C, 5000rpm, centrifuge for 10min, collect the supernatant; filter the supernatant through a 0.22μm microporous membrane, adjust the pH to 6.2 with sodium hydroxide; incubate 20μL for 24h with a concentration of 1×10 8 CFU / mL indicator bacteria solution (Escherichia coli, Salmonella typhimurium, Shigella flexneri) was added to the LB solid medium sterilized by high temperature and high pressure and cooled to about 45°C, mixed vigorously and quickly poured into a plate After the cultur...

Embodiment 3

[0050] Effects of Lactobacillus helveticus KM7 on the growth and intestinal health of adult worker bees of Apis mellifera

[0051] (1) Effect of Lactobacillus helveticus KM7 on the mortality of adult worker bees of Apis mellifera

[0052] 300 healthy adult worker bees of Apis mellifera were captured from the apiary of Yunnan Agricultural University and randomly divided into two groups. 8 CFU / mL Lactobacillus helveticus KM7 sugar water; the control group and the experimental group were raised in two self-made incubators respectively, and sufficient sugar and water supply were ensured during the experiment; on the 3rd, 6th, and 9th days of the experimental period, The bee mortality of the control group and the experimental group were counted respectively. Experimental results such as figure 1 As shown, with the increase of experimental age, the mortality of bees in the experimental group and the control group gradually increased; but on the 3rd, 6th, and 9th day, the mortalit...

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Abstract

The invention provides lactobacillus helveticus and application thereof in the bee breeding process. The lactobacillus helveticus strain is preserved in the China General Microbiological Culture Collection Center on July 2, 2018, has a preservation number of CGMCC No.16042, and is classified and namely as lactobacillus helveticus KM7. After the lactobacillus helveticus is cultured in an MRS agar culture medium, the lactobacillus helveticus, and has a round, milky white, neat-edged, smooth-faced, raised and non-transparent colonial form. The lactobacillus helveticus KM7 is spontaneously separated from intestines of Eastern worker bees which are cultured in the bee farm of the bee science department of animal science and technology college of the Yunnan Agricultural University in Kunming, Yunnan Province and freely digest flower honey in spring, has relatively good bacteriostatic activity, and is sensitive to multiple antibiotics. The lactobacillus helveticus KM7 is added into syrup feeding bees in an amount of 108CFU/mL, so that the floral microecological structure in the intestines of bees can be improved, the intestinal immunity of bees can be enhanced, and the survival rate of bees can be improved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a strain of Lactobacillus helveticus and its application in the bee breeding process, in particular to the application of a Lactobacillus helveticus KM7 strain in the bee breeding process. Background technique [0002] Honey bee is a highly social insect, which plays an important role in the process of constructing and maintaining natural ecosystems and ecological agriculture, and supplying human with advanced nutrition and health care products. my country is a big beekeeping country, as well as a big country in the production and export of bee products. The amount of honey exported each year accounts for about 20% of the world's total exports, and the average annual foreign exchange earnings from exports reach 140 million US dollars. However, during the breeding process, bees are susceptible to infectious diseases that are threatened by various external conditions, resulting in the de...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K10/18A23K50/90C12R1/225
CPCA23K10/18A23K50/90C12N1/205C12R2001/225
Inventor 林秋叶汪思凡曹振辉叶朋飞潘洪彬雷清芝郭文清黄丝艳钱颖艳余行罗程
Owner YUNNAN AGRICULTURAL UNIVERSITY
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