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A Schisandra chinensis vine stem extract has new application in preparing medicine and health product

A technology of Schisandra chinensis and extract, which is applied in the field of medicine, can solve the problem of less research on the pharmacological effects of triterpenoids and other compounds, and achieve the effect of expanding application channels

Inactive Publication Date: 2019-01-18
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Lignans, terpenes, volatile oils and polysaccharides in Schisandra chinensis are the main chemical components, among which lignans are rich in content and considered to be the main active components. There are many chemical components in Schisandra chinensis, and it is also the main component of Schisandra chinensis. There are research reports: the extract of Schisandra chinensis cane is rich in components and has less toxicity (see: Hu Yanwu, et al. Schisandra cane extract against liver fibrosis in rats Discussion on the role and mechanism, "Chinese Journal of Experimental Formulas", 2016; Liu Junxia, ​​et al. Insecticidal activity of extracts from different parts of Schisandra chinensis, "Northern Horticulture", 2017). Studies have shown that the extract of Schisandra chinensis contains a large amount of lignans, It also contains polysaccharides, flavonoids and other compounds. At present, the research on Schisandra chinensis is mostly focused on lignans and polysaccharides at home and abroad. The invention is mainly based on the fact that the cane extract of Schisandra chinensis contains rich ingredients. After an effective extraction method, the extract of schisandra cane with rich ingredients can be obtained, and it is found that it has obvious protective effect on liver damage

Method used

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  • A Schisandra chinensis vine stem extract has new application in preparing medicine and health product
  • A Schisandra chinensis vine stem extract has new application in preparing medicine and health product
  • A Schisandra chinensis vine stem extract has new application in preparing medicine and health product

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0024] Experimental Example 1. The preparation method of Schisandra cane extract

[0025] Preparation method 1: Take 10g of Schisandra cane, wash, crush, pass through a 40-mesh sieve, use ultrasonic-assisted extraction method, use 64% ethanol, the ratio of solid to liquid is 1:60, ultrasonically extract for 48min, extract 3 times, and combine the extracts , concentrated under reduced pressure to obtain Schisandra cane extract.

[0026] Preparation method 2: Take 10 g of Schisandra cane, wash, crush, pass through a 40-mesh sieve, and use supercritical CO 2 The extraction method extracts the lignans in the cane of Schisandra chinensis, and when the temperature is 50°C and the pressure is 27MPa, the extract of the cane of Schisandra chinensis is obtained. The extraction rate of its triterpenes is about 2.1 times that of ethanol solvent extraction.

[0027] Preparation method 3: Take 10 g of Schisandra cane, wash, crush, pass through a 40-mesh sieve, use a high-voltage pulse ele...

experiment example 2

[0028] Experimental Example 2. Analysis of Active Components in Schisandra Cane Extract

[0029] The active ingredients in the extract of Schisandra chinensis were measured by UV and HPLC, and the results showed that the extract contained Schisandrin A, Schisandrin B, Schisandrin A, and Schisandrin B; see attached figure 1 . Through UV and HPLC / MS analysis, see attached table 1.

[0030]The HPLC method is: Agilent liquid chromatography, using Phenomenes XB-C18 chromatographic column (250 mm×4.6 mm, 5 μm); mobile phase: acetonitrile (A)-water (B), gradient elution (0~10 min , A is 50%; 10~15 min, A is 50%~75%; 15~25min, A is 75%; 25~30 min, A is 75%~50%)); detection wavelength: 217 nm; flow rate: 1.0m L / min; Column temperature: 30℃; Injection volume: 10μL.

[0031] Attached Table 1: Comparison of Main Chemical Components of Schisandra Cane Extract

[0032]

experiment example 3

[0033] Experimental example 3. The protective effect of Schisandra chinensis supercritical CO2 extraction method on mice with hepatotoxicity induced by acetaminophen

[0034] Male ICR mice after adaptive feeding for 1 week were randomly divided into 5 groups, namely blank control group, model group, preparation method 2 of Example 1 Schizandra cane extract (SCE) low-dose group (200 mg·kg -1 ), middle dose group (300mg·kg -1 ) and high dose group (400mg·kg -1 ).

[0035] The administration group was administered by intragastric administration. For 7 consecutive days, the blank control group and the model group were given equal volumes of normal saline. One hour after the last administration, all groups except the blank group were given a one-time intraperitoneal injection of acetaminophen ( APAP) 250mg / kg, all mice were deprived of food and water the night before modeling and dissection. Mice were killed by cervical dislocation, and blood was collected from the ocular venous...

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Abstract

The invention belongs to the technical field of medicine and mainly discloses the preparation of Schisandra chinensis vine stem extract and the application thereof in the treatment of liver injury. The experimental results show that the extract of Schisandra chinensis vine stem is economical, time-saving, suitable for technological production, and easy to use. Pharmacodynamic studies have shown that the extract has significant protective effects on mice with severe liver injury and liver failure induced by paracetamol (also known as acetaminophen) overdose. Therefore, the active ingredient canbe used to prepare medicine, health product, food additive, compound preparation and the like for protecting liver. The medicine or health product can be any existing dosage form, such as tablet, capsule, powder injection, injection, pill, soft capsule, granule and patch, etc.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to the preparation of a schisandra cane extract and its application in treating liver damage. Background technique [0002] Acetaminophen (APAP) is a commonly used antipyretic and analgesic drug, which is safe in a certain therapeutic dose, but excessive APAP can cause severe liver damage and liver failure. APAP is not only a common cause of poisoning, It is also the cause of acute liver disease in patients with liver failure, mainly due to the excessive production of N-acetyl-p-benzoquinone imine, which causes mitochondrial dysfunction, and leads to liver necrosis or liver damage. At present, it is found in traditional Chinese medicine A variety of active ingredients have obvious preventive and therapeutic effects on liver damage. [0003] Schisandra chinensis Schisandra chinensis (Turcz.) Dry ripe fruit of Baill. It was first recorded in "Shen Nong's Materia Medica" and listed a...

Claims

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Application Information

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IPC IPC(8): A61K36/79A61K36/57A61P1/16A61K135/00
CPCA61K36/57A61K36/79A61K2236/333A61P1/16
Inventor 李伟王梓汤立民任珅李新殿李艳姿张伟哲
Owner JILIN AGRICULTURAL UNIV
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