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A kind of phthalate ester and its preparation method, bacterial strain and application

A technology of phthalates and seeds, applied in the field of symbiotic fungus - anthracnose, can solve the problems of unstable target products and difficult waste liquid treatment, and achieve the effects of short cycle, guaranteed source and low cost

Active Publication Date: 2021-02-02
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the production of phthalate is mainly chemical synthesis, which is formed by esterification of phthalic anhydride and alcohol under the catalysis of acid. Although the yield is high, there are side reactions such as dehydration and oxidation, and the target product is not Stability, difficult waste liquid treatment and other issues

Method used

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  • A kind of phthalate ester and its preparation method, bacterial strain and application
  • A kind of phthalate ester and its preparation method, bacterial strain and application
  • A kind of phthalate ester and its preparation method, bacterial strain and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: the isolation and identification of ball anthracnose bacterium (Colletotrichum coccodes) ZZP-L1

[0026] 1. Isolation of strain ZZP-L1

[0027] The final concentration of the slant medium (ie PDA medium) consists of: sucrose 20g / L, potato 200g / L, agar 20g / L, the solvent is distilled water, and the natural pH value.

[0028] The final concentration of the seed medium (ie PDB medium) consists of: 200 g / L of potatoes, 20 g / L of sucrose, distilled water as a solvent, and natural pH.

[0029] Strain ZZP-L1 was first isolated from the coastal plant Rumexobtusifolius Linn. by Mr. Zhang Huawei from Zhejiang University of Technology using common symbiotic bacteria isolation techniques (for details, refer to Bangladesh Journal of Pharmacology, 2012, 7:120-123), and was inoculated in Inclined media.

[0030] 2. Identification of strain ZZP-L1

[0031] (1) Morphological characteristics: strain ZZP-L1 was inoculated on PDA medium, activated at 28°C for 3-4 days, and...

Embodiment 2

[0035] Embodiment 2: the preparation of globular anthracnose bacteria ZZP-L1 fermentation medium

[0036] (1) Slant culture: inoculate the slant culture medium with Bacillus anthracis ZZP-L1, and culture in a 28°C incubator for 3-4 days to obtain a slant surface; the final concentration composition of the slant medium is the same as in Example 1.

[0037] (2) Seed culture: pick an inoculation ring from the slant of the thalline and inoculate it into the seed medium, and culture it on a shaker at 200 rpm and 28°C for 3 days to obtain the seed liquid; the final concentration of the seed medium is composed with the same implementation example 1.

[0038] (3) Fermentation culture: the seed liquid obtained in step (2) is inoculated to the fermentation medium with an inoculum size of 10% volume concentration, and cultured on a shaker at 200 rpm and 28° C. for 10 days to obtain a fermentation medium, a fermentation medium The final concentration is the same as that of the seed mediu...

Embodiment 3

[0039] Embodiment 3: Extraction and separation, identification of two kinds of phthalates

[0040] 1. Extraction and separation of two phthalates

[0041] After the fermentation broth obtained in Example 2 was filtered, the filtrate was extracted twice at room temperature with ethyl acetate (volume ratio 1:1), and the upper organic phase was taken and concentrated in vacuum at low temperature (35°C, 0.01MPa) until no liquid flowed out. Dry to obtain brown crude extract F; the methanol solution of crude extract F is separated by reversed-phase high-performance liquid chromatography (RP-HPLC), and the peaks at the 6th and 14th minutes are collected respectively, and the mobile extract F is removed under low-temperature vacuum (35°C, 0.01MPa). Phase to obtain compound (I), compound (II).

[0042] Liquid phase instrument: UV-VIS, detector: Shimadzu SPD-10AV; HPLC infusion pump: Shimadzu LC-10AD; chromatographic conditions: C 18 The chromatographic column is 250×9.4mm, the flow r...

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Abstract

The invention discloses a phthalate and its preparation method, bacterial strain and application. The fermented liquid obtained by fermenting and cultivating Bacillus anthracis ZZP-L1 is filtered or centrifuged, and the filtrate or supernatant is obtained with ethyl acetate or Dichloromethane extraction, take the organic layer and concentrate until no liquid flows out, the concentrate is separated and purified by reverse-phase high-performance liquid chromatography, the peaks at the 6th and 14th minutes are collected respectively, and the solvent is removed to obtain formula (I) or formula (II) Phthalates indicated. The phthalate (I) of the present invention has inhibitory effect on Candida albicans (Candida albicans), can be used as a new type of antibiotic, and is expected to be applied to the preparation of medicines for treating related diseases caused by Candida albicans; two phthalates of the present invention The phthalate is produced from the liquid fermentation of Bacillus anthracnose ZZP-L1, the operation process is simple, the cycle is short, the cost is low, and the source is guaranteed; the invention utilizes a biological method to synthesize and has no pollution to the environment.

Description

[0001] (1) Technical field [0002] The invention relates to two kinds of phthalates, their biological preparation method and application, and the symbiotic fungus producing the phthalates—Colletotrichum coccodes ZZP-L1. [0003] (2) Background technology [0004] The discovery of new lead compounds of antibacterial drugs has always been a hot spot for medicinal chemists. Plant symbionts are a special kind of microorganisms, which live between cells or in tissues of healthy plant tissues, do not cause any obvious disease symptoms of host plants, and form a mutualistic symbiotic relationship with the host. A large number of chemical studies have shown that plant symbiotic fungi have rich chemical diversity and can metabolize compounds with unique chemical structures and significant biological activities. The living environment of coastal plants is very special, and their symbiotic fungi have different survival and metabolic mechanisms from terrestrial microorganisms. In-depth ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C69/80C12P7/62C12N1/14A61P31/10C12R1/645
CPCA61P31/10C07C69/80C12P7/62C12N1/145C12R2001/645
Inventor 章华伟张玮月王鸿华熠潘瑞
Owner ZHEJIANG UNIV OF TECH