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A kind of Issa orientalis strain zt-c2 and its application

A technology of ZT-C2 and Isaac yeast, applied in the field of environmental microorganisms, to achieve the effects of high activity, high bacterial activity, and high desulphurization and denitrification efficiency

Active Publication Date: 2021-08-10
NANJING AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The purpose of the present invention is to provide a new type of yeast, which belongs to the Saccharomyces saccharomyces family, to solve the problem that the odor gas produced by hydrogen sulfide and ammonia in the waste water brings troubles to people's lives

Method used

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  • A kind of Issa orientalis strain zt-c2 and its application
  • A kind of Issa orientalis strain zt-c2 and its application
  • A kind of Issa orientalis strain zt-c2 and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Example 1 Screening and identification of sulfur and denitrification bacterial strain ZT-C2

[0033] PDA medium: Potato dextrose agar medium (PDA): 20.0g glucose, 200.0g potatoes (cooked and filtered, filtrate required), 15.0g agar powder, add distilled water to 1000mL, natural pH, sterilized at 115°C for 30min.

[0034] Ammonia enriched medium: 5.0 g sucrose, 2.0 g peptone, potassium dihydrogen phosphate (KH 2 PO 4 ) 2.0g, magnesium sulfate (MgSO 4 ·7H 2 O) 0.5g, sodium chloride (NaCl) 2.0g, zinc sulfate (ZnSO 4 ) 0.05g, ferrous sulfate (FeSO 4 ) 0.04g, ammonia water 5.0mL (filter sterilized, add after sterilization), add agar powder 15.0g when coating and purify, add distilled water to 1000mL, pH=7.0, sterilize at 121°C for 20min.

[0035] Sulfur enriched medium: ammonium chloride (NH 4 Cl) 2.0g, magnesium chloride (MgCl 2 ) 0.5g, dipotassium hydrogen phosphate (K 2 HPO 4 ·3H 2 O) 3.0g, sodium sulfide (Na 2 S·9H 2 O) 10.0g (filter sterilized, add after ste...

Embodiment 2

[0052] The growth curve of embodiment 2 Issahia orientalis ZT-C2

[0053] to pick figure 1 The single colonies shown were cultured in PDA liquid medium with shaking at 28° C. for 12 hours to obtain fresh seed liquid. Take 6 50ml Erlenmeyer flasks with 20ml liquid PDA medium, labeled 01, 02, 03, ZT-C2-1, ZT-C2-2, ZT-C2-3. ZT-C2-1, ZT-C2-2, and ZT-C2-3 are the experimental groups, and the experimental group is injected with 100 μL of seed liquid into the Erlenmeyer flask according to the inoculated amount of 1%. The blank group was added with the same volume of sterile water, and one group was repeated three times. From 0 hour, the absorbance value A at 560 nm was measured with a spectrophotometer every hour. A blank group was used as a blank control. The 36-hour growth curve of sulfur and denitrification bacterial strain ZT-C2 in PDA was obtained. Such as figure 2 As shown, 12 hours has reached the optimum value.

Embodiment 3

[0054] The optimum pH of embodiment 3 strain ZT-C2 and the mensuration of optimum temperature

[0055] to pick figure 1 The single colonies shown were cultured in PDA liquid medium with shaking at 28° C. for 12 hours to obtain fresh seed liquid. Get 24 test tubes of 5ml liquid PDA medium, adjust its pH with a pH meter, and every three test tubes are the scope of repeated pH of a pH being 4-10. Medium at normal pH served as a control. In the experimental group, 100 μL of seed solution was added to the Erlenmeyer flask according to the inoculation amount of 1%. The blank group was added with the same volume of sterile water. After 8 hours, the absorbance value at OD560 was measured, and the blank group was used as a blank control. Obtain the optimum pH between pH=4-10 of sulfur and denitrification bacterial strain ZT-C2. image 3 shown. The optimal pH of the strain ZT-C2 was between 5-9.

[0056] to pick figure 2 The single colonies shown were cultured in PDA liquid med...

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Abstract

The invention discloses a strain ZT-C2 of Issaccharomyces orientalis and its application. The sulfur-removing and denitrifying bacteria ZT-C2 was preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee on June 25, 2018, and the strain preservation number is It is CGMCC NO.16008, named as: Issatchenkia orientalis, address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, Zip code: 100101. After testing, the strain was inoculated into sewage, and the removal rate of hydrogen sulfide in water reached more than 60% in 12 hours, and the removal rate of hydrogen sulfide in water reached more than 80% in 24 hours, and the removal rate of ammonia in sewage reached 60% after 20 days About 23 days later, the removal rate of ammonia in wastewater is close to 70%, which further shows that the strain has high activity, strong adaptability, and high efficiency of sulfur and nitrogen removal.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and in particular relates to an Issaccharomyces orientalis strain ZT-C2 capable of removing sulfur and nitrogen and its application in the aspect of sulfur and nitrogen removal in wastewater. Background technique [0002] With the continuous development of the economy, the continuous advancement of the urbanization process, and the continuous improvement of people's living standards, municipal treatment facilities such as sewage treatment plants, sewage lifting stations, garbage transfer stations, and landfills are getting closer and closer to people's living areas. The black and smelly sewage generated during the operation of these facilities has become an important factor affecting people's normal life. The main cause of black and odorous sewage is eutrophication due to excessive ammonia nitrogen content, which increases the organic matter content in the water body. Organic matter m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C02F3/34C12R1/645C02F101/16
CPCC02F3/34C02F2101/101C02F2101/16C12N1/14C12N1/145C12R2001/645
Inventor 钟增涛曹亚君江高飞马东燕尹显燕
Owner NANJING AGRICULTURAL UNIVERSITY
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