Core-shell nano-rod with ferroferric oxide coated with nickel-loaded carbonization layer and preparation method and application of core-shell nano-rod

A technology of ferroferric oxide and nanorods, which is applied in the field of polymer materials and biomedicine

Active Publication Date: 2019-02-15
SHANGHAI UNIV OF ENG SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Despite their high sensitivity, these methods often req

Method used

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  • Core-shell nano-rod with ferroferric oxide coated with nickel-loaded carbonization layer and preparation method and application of core-shell nano-rod
  • Core-shell nano-rod with ferroferric oxide coated with nickel-loaded carbonization layer and preparation method and application of core-shell nano-rod
  • Core-shell nano-rod with ferroferric oxide coated with nickel-loaded carbonization layer and preparation method and application of core-shell nano-rod

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] Example 1 Fe 3 o 4 Synthesis of @C-Ni

[0078] (1) Preparation of Fe-NTA complex

[0079] Dissolve 2.6g of ammonium ferrous sulfate hexahydrate and 0.6g of nitrilotriacetic acid in 40mL of water evenly, and react at a constant temperature of 180°C for 720min. After cooling down to room temperature, filter and collect the precipitate, and wash it alternately with ethanol and distilled water several times to obtain a white The precipitate is Fe-NTA.

[0080] (2) Fe 3 o 4 Preparation of @C-Ni

[0081] Dissolve 200mg of Tris in 5mL of water evenly to form A solution.

[0082] 15 mg of dopamine (DA) and 37.6 mg of nickel chloride hexahydrate were uniformly dissolved in a mixed solution consisting of 2 mL of ethanol and 1 mL of water to form solution B.

[0083] Dissolve 50mg of Fe-NTA uniformly in a mixed solution consisting of 30mL of absolute ethanol and 20mL of water to form solution C.

[0084] Under the condition of stirring, add solution A and solution B dropwi...

Embodiment 2

[0087] Example 2 Fe 3 o 4 Peroxidase-like activity of @C-Ni

[0088] 1. Peroxidase-like activity

[0089] (1) Take 285 μL, 290 μL, 290 μL, 295 μL of 0.2M pH 4.0 acetic acid-sodium acetate buffer solution in each centrifuge tube, add 6 μL, 0 μL, 6 μL, 0 μL Fe 3 o 4 @C-Ni aqueous solution (5mg / mL), 6μL, 6μL, 0μL, 0μL hydrogen peroxide aqueous solution (0.01M), 3μL, 3μL, 3μL, 3μL 3,3',5,5'-tetramethylbenzidine anhydrous Ethanol solution (TMB, 10mM), mix the above solution evenly;

[0090] (2) reacting the mixed solution prepared in step (1) at room temperature for 10 min;

[0091] (3) By applying an external magnetic field, Fe 3 o 4 @C-Ni is separated from the reaction solution;

[0092] (4) Measure the ultraviolet absorption spectrum of the above mixed solution with an ultraviolet-visible absorption spectrophotometer.

[0093] The result is as figure 2 As shown, Fe 3 o 4 @C-Ni+TMB+H 2 o 2 The UV absorbance is about TMB+H 2 o 2 Twice as much as Fe 3 o 4 Four ti...

Embodiment 3

[0112] Example 3 Fe 3 o 4 Kinetic experiment of @C-Ni

[0113] 1.H 2 o 2 experiment

[0114] [TMB]=0.20mM

[0115] (1) Take 5 parts of 290 μL 0.2M pH=4.00 acetic acid-sodium acetate buffer solution in parallel in each centrifuge tube, and add 6 μL Fe 3 o 4 @C-Ni aqueous solution (1mg / mL), hydrogen peroxide aqueous solution with different concentrations (50, 100, 200, 500, 1000 μM for final concentration), 3 μL 3,3',5,5'-tetramethylbenzidine Anhydrous ethanol solution (TMB, 20mM), mix the above solutions evenly, and the concentration of TMB in each mixed solution prepared in parallel is 0.2mM;

[0116] (2) React the mixed solution prepared in step (1) in a 60°C water bath for 10 minutes;

[0117] (3) By applying an external magnetic field, Fe 3 o 4 @C-Ni is separated from the reaction solution;

[0118] (4) measure the ultraviolet absorption spectrum of above-mentioned mixed solution with ultraviolet-visible absorption spectrophotometer, take the reciprocal of differ...

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Abstract

The invention belongs to the field of high polymer materials and biological medicines, and particularly relates to a core-shell nano-rod with ferroferric oxide coated with a nickel-loaded carbonization layer and a preparation method and application of the core-shell nano-rod. The core-shell nano-rod comprises a ferroferric oxide nano-rod core and a nickel-loaded carbonization layer shell which wraps the ferroferric oxide nano-rod. A Fe-NTA complex is chelated with nickel salt and dopamine and then is calcined to prepare the core-shell nano-rod. The core-shell nano-rod has catalytic activity which is similar to that of peroxidase, can be used for preparing analogous peroxidase or peroxidase analogue enzyme, can further be used for preparing an electrochemical sensor which converts DNA signal detection into electric signal detection by a bridge effect of a biotin-avidin system, and is high in selectivity and good in specificity, detecting results are visual, and are easy to count accurately, and furthermore, the core-shell nano-rod is convenient and time-saving, and is high in precision and good in accuracy.

Description

technical field [0001] The invention belongs to the field of macromolecular materials and biomedicine, and in particular relates to a core-shell nanorod with a nickel-negative carbide layer coated with iron tetroxide and a preparation method and application thereof. Background technique [0002] Sensitive and efficient detection of trace amounts of sequence-specific DNA is of great importance in clinical diagnosis, food analysis, bioterrorism, and environmental monitoring. Commonly used DNA detection techniques include fluorescent colorimetric detection, molecular imprinting, polymerase chain amplification reaction (PCR) and DNA microarray, but the application of these methods is often limited by complex experimental steps, expensive instruments and equipment, and Limited sensitivity, some methods are difficult to detect low-abundance nucleic acid samples with high sensitivity and low cost. [0003] In recent years, many methods have been proposed to replace PCR technology ...

Claims

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Application Information

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IPC IPC(8): G01N27/327
CPCG01N27/308G01N27/3276G01N27/3277G01N27/3278
Inventor 曾彩霞鲁娜张敏王娜张锐张佳星
Owner SHANGHAI UNIV OF ENG SCI
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