A kind of liposome preparation method

Abstract

The invention discloses a preparation method of liposome. The method includes the following steps: firstly, synthesizing a diethylenetriamine pentaacetic acid derivative; secondly, synthesizing gadolinium-diethylenetriamine pentaacetic acid-stearamine with amphiphilic molecules; thirdly, forming a liposome shell layer, performing drug self-assembly; and finally, preparing a liposome material. Theliposome with a core-shell structure is prepared by a self-assembly and a nano-precipitation method, and has simple process and strong repeatability; and moreover, the formed liposome has uniform particle size, good water solubility, dispersibility and biocompatibility, overcomes the defects of poor biocompatibility, low tissue specificity, large toxicity and the like of the gadolinium small molecules, and simultaneously utilizes near-infrared light irradiation to indirectly cause phase transformation of fatty acids, controllably releases chemotherapeutic drugs, and realizes multimodal imaging-guided cooperative treatment.

Description

technical field [0001] The invention relates to a method for preparing liposomes, in particular to a method for preparing liposomes with multifunctional properties, and belongs to the field of biomedicine. Background technique [0002] Magnetic resonance imaging (MRI) technology is an imaging technology derived from the magnetic resonance effect of atomic nuclei. It has the characteristics of high spatial resolution, high soft tissue contrast, non-invasiveness and no ionizing radiation damage, so it is widely used in clinical diagnosis and treatment. middle. In clinical MRI, most diagnoses must use magnetic resonance contrast agent, which is an imaging enhancement contrast agent used to shorten imaging time, improve imaging contrast and clarity, and it can change the relaxation of water protons in local tissues in the body. Improve the imaging contrast of normal and diseased parts. The development of magnetic resonance contrast agent also promotes the maturity of MRI techn...

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Problems solved by technology

[0003] In addition, in recent years, many multifunctional nanoprobes that release drugs in response to near-infrared have been developed for the synergistic treatment of cancer, such as chemotherapy or phototherapy, but these nanoprobes can only achieve persistent drug-encapsulation under near-infrared stimulation. Release, but can not achieve controllable and on-demand release, resulting in low lesion specificity, poor therapeutic effect and other problems

[0004] However, currently the most clinically used contrast agents, such as gadolinium-based contrast agents, gadodiamide (Gd-DTPA-BMA,) 1,4,7-tris(tert-butoxycarbonylmethyl)-10-(acetic acid)-1 ,4,7,10-Tetraazacyclododecane (Gd-DOTA) etc. are small molecular contrast agents, which belong to extracellular reagents, and have disadvantages such as low tissue specificity, short residence time in vivo, and high tissue toxicity.

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