Method for extracting undaria pinnatifida sulfated polysaccharide and application thereof in regulating intestinal flora

A technology for regulating intestinal flora and sulfated polysaccharides, which is applied in the field of food processing, can solve problems such as endangering personal safety and environmental pollution, and achieve the effects of improving purity, reducing volume, and facilitating dialysis and freeze-drying

Active Publication Date: 2019-03-01
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The current patent CN107417807A uses ultrasonic method and organic solvents such as ethyl acetate and ethanol to extract seaweed polysaccharides, but the ethyl acetate used is a toxic and harmful reagent that will endanger personal safety and cause environmental pollution. In the patent CN107522795A, a high-speed homogenizer is used to extract seaweed sulfuric acid polysaccharides, but the organic reagents used in the extraction are not recycled, which may cause environmental pollution

Method used

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  • Method for extracting undaria pinnatifida sulfated polysaccharide and application thereof in regulating intestinal flora
  • Method for extracting undaria pinnatifida sulfated polysaccharide and application thereof in regulating intestinal flora

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: Preparation of Undaria pinnatae sulfate polysaccharide

[0034] S1, pulverize Undaria pinnatae (water content 3%~4%) into powder and pass through a 100-mesh sieve to obtain Undaria pinnatifida powder; weigh 70g of Undaria pinnatifida powder, add 1.75 L water, adjust temperature to 50 ℃ and utilize citric acid (1mol / l)-disodium hydrogen phosphate (2mol / l) buffer to adjust pH value to 4.8, add 14000U cellulase (the enzyme activity of described cellulase ≥ Stir at a constant speed of 50 U / mg) at 40 rpm / min for 15 min; then adjust the temperature to 45°C and pH to 4, add 7000 U of pectinase (the enzyme activity of the pectinase is ≥100 U / mg) and stir at a constant speed of 40 rpm / min for 1.5 h; Finally, adjust the temperature to 50°C and the pH value to 5.5, add 140000U papain (the enzyme activity of the papain is ≥2000U / mg) and stir at a constant speed of 40rpm / min for 1.5h, and wait for the mixture;

[0035] S2. Heat the mixed solution obtained in step S1 t...

Embodiment 2

[0043] Embodiment 2: Preparation of Undaria pinnatae sulfate polysaccharide

[0044] S1, pulverizing Undaria pinnatifida (water content 3%~4%) into powder and passing through a 100-mesh sieve to obtain Undaria pinnatifida powder;

[0045] Weigh 2000g of wakame powder, according to the ratio of material to liquid 1:30 (m / v), add 60L of water, adjust the temperature to 60°C and use citric acid (1mol / l)-disodium hydrogen phosphate (2mol / l) buffer Adjust the pH value to 5.2, add 440000U cellulase (enzyme activity ≥ 50U / mg) and stir at a constant speed of 60rpm / min for 25min; then adjust the temperature to 55°C and pH value to 4.4, add 280000U pectinase (enzyme activity ≥ 100U / mg ) at 60rpm / min for 2.5h at a constant speed; finally adjust the temperature to 60°C and the pH value to 6.5, add 5600000U papain (enzyme activity ≥ 2000U / mg) and stir at 60rpm / min for 2.5h at a constant speed to obtain a mixture;

[0046] S2. Heat the mixed solution obtained in step S1 to 95° C. for 10 minu...

Embodiment 3

[0053] Example 3: Modulation of intestinal flora using wakame sulfated polysaccharides

[0054] (1) 36 BALB / c mice of the same batch (purchased from Dalian Medical University) were selected and divided into 3 groups, namely the normal group, the high-fat group and the polysaccharide group; the mice were given normal saline based on their body weight , undaria sulfate polysaccharide, normal group and high-fat group mice every day each gavage 500mg / Kg of normal saline, polysaccharide group mice every day each gavage wakame sulfate polysaccharide of 500mg / Kg, in the above three groups of mice The normal group was fed with normal feed, and the high-fat group and polysaccharide group were both fed with high-fat feed. After seven weeks, they were killed, and the contents of the colon were taken to extract DNA, and the microbial composition was analyzed by high-throughput sequencing; wherein, the wakame sulfate The polysaccharide was prepared by the embodiment of the present inventio...

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Abstract

The invention discloses a method for extracting undaria pinnatifida sulfated polysaccharide. The method includes utilizing undaria pinnatifida powder as a raw material and then conducting enzymatic hydrolysis, enzyme deactivation, sodium alginate removal, CTAB precipitation, dissolution, concentration and desalting and lyophilization to obtain the undaria pinnatifida sulfated polysaccharide. The extraction rate of the undaria pinnatifida sulfated polysaccharide is 0.45%-0.55%. Compared with the other methods, the method achieves the higher extraction rate, and the purity of the obtained undaria pinnatifida sulfated polysaccharide is better, being 86.42%-88.59%. The undaria pinnatifida sulfated polysaccharide can be used for regulating intestinal microorganisms, especially for improving theproportion of firmicutes and bacteroides in the intestinal tract.

Description

technical field [0001] The invention belongs to the technical field of food processing, and relates to extraction technology of undaria pinnatiflora sulfated polysaccharide, in particular to a method for extracting undaria pinnatifera sulfated polysaccharide and its application in regulating intestinal flora. Background technique [0002] Wakame (Undaria pinnatifida) is mainly produced in coastal areas such as Liaoning Province, Zhejiang Province and Shandong Province in China. The output is large, but the related products are mainly concentrated in rough processed products such as ready-to-eat and dried products, lacking in deep-processed products. Therefore, it is of great significance to extract polysaccharides from wakame and improve its economic benefits and resource utilization. [0003] The main active ingredient of wakame is polysaccharide. Sulfated polysaccharides in Wakame have good anti-virus, anti-tumor and immune-regulating effects, but there is no report on t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P1/00
CPCA61P1/00C08B37/0003C08B37/006
Inventor 艾春青罗腾瑞刘伊莉姜平芮姜国平宋爽
Owner DALIAN POLYTECHNIC UNIVERSITY
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