Preparation method and application of microorganism feed for milk thistle silybum marianum meal

A technology of microbial feed and silymarin meal, which is applied in the field of preparation of silymarin meal microbial feed, can solve the problems of low added value, poisoning of monogastric animals, activity discount of bacterial enzymes and functional substances, etc., and improve the overall nutritional level , promote the maturation of immune organs, and improve the effect of feed utilization

Inactive Publication Date: 2019-03-15
ZHONGXING PHARM CO LTD JIANGSU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Milk thistle meal is the residue after extracting silymarin from traditional Chinese medicine factories. In most cases, it will be discarded and pollute the environment. In some cases, it will be bought at a low price for cattle and sheep farmers, but it is difficult to get better utilization.
In fact, milk thistle meal also contains more than 20% protein, rich in carbohydrates, including more than 20% starch, 30% to 40% crude fiber, especially more than 1% total flavonoid residue, if it is applied to animal The use value of the feed is significant; however, according to most of the current methods, it is directly added to animals in a certain proportion, which has the problems of poor palatability, low digestion and a

Method used

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  • Preparation method and application of microorganism feed for milk thistle silybum marianum meal
  • Preparation method and application of microorganism feed for milk thistle silybum marianum meal
  • Preparation method and application of microorganism feed for milk thistle silybum marianum meal

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A preparation method for milk thistle meal microbial feed, comprising the steps of:

[0034] (1) Phase I solid-state fermentation:

[0035] 1-A) Preparation of compound milk thistle meal mixture:

[0036] Prepare compound milk thistle meal by the ratio of 40% milk thistle meal, 25% vinegar residue, control water content is 35%; Add the cellulase of 20g / t in compound milk thistle meal (enzyme activity is 1 10,000 u / g), 20g / t xylanase (enzyme activity is 180,000 u / g), 5g / t mannanase (enzyme activity is 10,000 u / g), 30g / t α- Galactosidase (enzyme activity is 500u / g), 20g / t acid protease (enzyme activity is 60,000u / g), 20g / t β-glucanase (enzyme activity is 50,000u / g), The phytase of 20g / t (enzyme activity is 100,000 u / g) and the calcium hydrogen phosphate of 0.2% by mass are prepared into compound milk thistle meal mixture;

[0037] 1-B) Cultivation of mixed bacillus liquid strains:

[0038] Under sterile conditions, add a ring of Bacillus licheniformis, Bacillus subtil...

Embodiment 2

[0060] Other implementations are the same as in Example 1, except that the inoculum size of the mixed bacillus liquid strain in step 1-D) is 10%, acid protease (enzyme activity is 60,000 u / g) in step 1-A) The added amount is 200g / t.

Embodiment 3

[0062] Other implementations are the same as in Example 1, except that the addition of cellulase (enzyme activity is 10,000 u / g) in step 1-A) is 200g / t; xylanase (enzyme activity is 180,000 u / g) u / g) is added in an amount of 200g / t mannanase (enzyme activity is 10,000u / g) is added in an amount of 20g / t; α-galactosidase (enzyme activity is 500u / g) is added in an amount 300g / t; β-glucanase (enzyme activity: 50,000 u / g) added at 200g / t.

[0063] Finished product analysis:

[0064] For embodiment 1-3 after the microbial feed finished product after two-stage anaerobic fermentation one month carries out sample detection, specifically as shown in table 1:

[0065] Table 1 Example 1-3 microbial feed finished product test

[0066]

[0067]

[0068] Result analysis:

[0069] As can be seen from Table 1, the acidity of a kind of milk thistle meal microbial feed prepared by Examples 1-3 of the present invention has dropped to 4.1 in terms of pH, which means that the quality has s...

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Abstract

The invention discloses a preparation method and application of a microorganism feed for milk thistle silybum marianum meal. The preparation method comprises the following steps of adding acid protease, cellulases, xylanase, mannase, alpha-galactosidase, beta glucanase and phytase to the milk thistle silybum marianum meal, adding calcium hydrogen phosphate, performing uniform stirring, and performing first-stage fermentation with bacillus and microzymes; and then adding lactic acid bacteria and bifidobacteria, and then performing second-stage anaerobic fermentation in a one-way membrane anaerobic bag to obtain finished products of the microorganism feed. The content of viable bacteria is increased, and the preparation raw materials are free from sterilization. The prepared products are free from drying treatment, and the production cost is reduced. The finished products of the microorganism feed contain rich functional substances of viable bacteria, enzyme groups, vitamins, lipid, organic acid, amino acids, various oligosaccharide and coenzyme factors, small peptide and the like, and are few in infectious microbes and obvious in feeding effects. The utilization efficiency of the feed is improved, the immunity and the resistance of the fed objects are effectively improved, the microorganism feed can be used for replacing antibiotics, the quality of animal products is further improved, the fetor of fence shed is eliminated, and the ecological benefits are obvious.

Description

technical field [0001] The invention relates to the field of feed preparation, in particular to a preparation method and application of milk thistle meal microbial feed. Background technique [0002] At present, the market scale of the feed industry is huge, and it is increasing at a rate of about 15% per year, but the profit is very low. Most of them are produced with grain as raw material, and the cost is difficult to reduce. It will be one of the future development directions to produce cheap and high-quality biological feed through a series of microbial treatments from non-grain raw materials. Microbial feed is an environmentally friendly and safe feed that has become popular in recent years. It is an active feed produced by probiotics as the main fermentation strain. The probiotic effects of feed utilization, health care and many other aspects can completely or partially replace antibiotics. [0003] Milk thistle meal is the residue after extracting silymarin from tra...

Claims

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Application Information

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IPC IPC(8): A23K10/12A23K10/37C12P1/02C12P1/04C12R1/07C12R1/01C12R1/645
CPCA23K10/12A23K10/37C12P1/02C12P1/04C12N1/145C12N1/205C12R2001/01C12R2001/07C12R2001/645Y02P60/87
Inventor 武俊明陈华友魏云王夕雯
Owner ZHONGXING PHARM CO LTD JIANGSU
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