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Preparation method of va@plga-cs-ha composite antibacterial slow-release microspheres and its application in scaffold materials for bone repair

A slow-release microsphere, compound antibacterial technology, applied in prosthesis, tissue regeneration, medical science and other directions, can solve the problems of difficult antibacterial concentration of local antibacterial drugs, difficult to achieve controlled release of drugs, etc., to achieve good biological safety, good clinical Application prospect, effect of suitable degradation rate

Active Publication Date: 2020-08-18
乐国平
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing slow-release bone repair materials still have defects such as the difficulty in achieving the effective antibacterial concentration of local antibacterial drugs and the controlled release of drugs. Therefore, there is an urgent need for bioactive antibacterial bone repair materials with controllable drug release and controllable degradation in clinical practice. , used to effectively repair bone defects caused by chronic bone infection, reduce the failure rate of surgery and the recurrence rate of infection

Method used

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  • Preparation method of va@plga-cs-ha composite antibacterial slow-release microspheres and its application in scaffold materials for bone repair
  • Preparation method of va@plga-cs-ha composite antibacterial slow-release microspheres and its application in scaffold materials for bone repair
  • Preparation method of va@plga-cs-ha composite antibacterial slow-release microspheres and its application in scaffold materials for bone repair

Examples

Experimental program
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Effect test

Embodiment 1

[0045] A method for preparing VA@PLGA-CS-HA composite antibacterial slow-release microspheres of this embodiment, the method includes the following steps:

[0046] (1) PLGA is dissolved in methylene chloride, is mixed with the PLGA solution that mass percentage concentration is 10wt%, for subsequent use; Chitosan is dissolved in the glacial acetic acid that mass percentage concentration is 2wt%, is mixed with mass percentage concentration and is 1wt% % chitosan solution, for subsequent use; sodium hyaluronate is dissolved in distilled water, and the preparation mass percentage concentration is the sodium hyaluronate solution of 1.5wt%, for subsequent use; vancomycin hydrochloride (VA) is dissolved in distilled water , the preparation mass percent concentration is respectively the vancomycin solution of 25mg / ml, 50mg / ml, 100mg / ml, for subsequent use; Polyvinyl alcohol (PVA) is dissolved in distilled water, and the preparation mass percent concentration is the PVA solution of 3wt...

Embodiment 2

[0054] The preparation method of the VA@PLGA-CS-HA composite antibacterial slow-release microspheres in this example is basically the same as the method in Example 1, the only difference is that the mass percent concentration of the PLGA solution in step (1) is 5wt%, and the shell The mass percentage concentration of the polysaccharide solution is 0.5 wt%, and the mass percentage concentration of the sodium hyaluronate solution is 1 wt%.

Embodiment 3

[0056] The preparation method of the VA@PLGA-CS-HA composite antibacterial slow-release microspheres in this example is basically the same as the method in Example 1, the only difference is that the mass percent concentration of the PLGA solution in step (1) is 5wt%, and the shell The mass percentage concentration of the polysaccharide solution is 1 wt%, and the mass percentage concentration of the sodium hyaluronate solution is 2 wt%.

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Abstract

The invention relates to a preparation method of VA@PLGA-CS-HA composite antibacterial sustained release microspheres and an application of the microspheres in a bone repair scaffold material and belongs to the field of biomedical materials. The preparation method of the microspheres comprises the steps as follows: firstly, uniformly mixing a PLGA solution and a VA solution to form a W1 / O emulsion; uniformly mixing a CS solution and PVA aqueous solution to obtain a CS / PVA solution; mixing a sodium hyaluronate solution and a PVA solution to prepare a HAS / PVA solution; then, mixing the CS / PVA solution with the W1 / O emulsion to obtain a stable W1 / O / W2 emulsion; mixing the W1 / O / W2 emulsion with the HAS / PVA solution, adding a sodium tripolyphosphate aqueous solution, finally, performing standing, centrifugation, washing and drying to prepare the microspheres. The method is simple and effective and has good biological safety, and the prepared microspheres have excellent antibacterial, degradation properties and the like and are suitable to be used as a bone defect repair material.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a preparation method of VA@PLGA-CS-HA composite antibacterial slow-release microspheres and its application in bone repair scaffold materials. Background technique [0002] With trauma, bone disease, tumor, congenital deformity and other causes of articular cartilage surface defects often appear in clinical work, but because of its very limited self-repair ability, it is almost impossible to repair by itself, so people's demand for cartilage tissue repair materials is increasing increase. At present, for the treatment of bone defects caused by chronic bone infection, the commonly used clinical methods include systemic use of antibiotic anti-infection therapy and local use of antibiotic slow-release system. However, due to scarring of local bone and soft tissue and poor blood supply in patients with chronic bone infection, systemic application of antibiot...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/22A61L27/20A61L27/12A61L27/18A61L27/50A61L27/54A61L27/58
CPCA61L27/12A61L27/18A61L27/20A61L27/222A61L27/50A61L27/54A61L27/58A61L2300/406A61L2430/02A61L2430/06C08L67/04C08L5/08
Inventor 乐国平赵劲民胡春蓉张明席立成
Owner 乐国平