Ginseng flower compound fermentation product and its preparation method and application
A technology of composite fermented products and ginseng flowers, which can be applied in the direction of pharmaceutical formulas, cosmetic preparations, dressing preparations, etc., can solve the problems of little increase in the content of rare ginsenosides and the inability to produce rare ginsenosides, achieving good application prospects, The effect of remarkable whitening effect and easy operation
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0026] Example 1 Preparation of ginseng flower composite fermentation product
[0027] (1) Prepare PBD medium, and add ginseng flower extract to make the concentration of ginseng flower extract 10g / L; cool after high temperature and high pressure sterilization; in the used ginseng flower extract, the total saponin content is 15% to 30% , which contains ginsenoside Re 7% to 15%, Rg1 0.5% to 2%, Rd 2% to 10%. The total sugar content is 30% to 60%, and the total polyphenol content is 0.5% to 2%.
[0028] (2) After activation, purification and expansion, the yeast grown to the logarithmic growth phase was inoculated into the medium containing the ginseng flower extract, and the inoculation amount was 5 mL / L.
[0029] (3) constant temperature culture on a shaker, culture at 34°C for 5 days, add MRS medium suitable for the growth of lactic acid bacteria after fermentation, and cool after high temperature and high pressure sterilization;
[0030] (3) the lactic acid bacteria grown ...
Embodiment 2
[0041] Example 2 Human keratinocyte proliferation experiment
[0042] Keratinocytes are the most important cells in the epidermis, which participate in the formation of the physical barrier of the skin, prevent the invasion of physical, chemical and microbial and other adverse factors, and play a protective role on the skin. One of the main features of skin aging is the thinning of the epidermis and the slower wound healing, which is mainly due to the decrease in the regeneration ability of keratinocytes in the epidermis and the decrease in the proliferation ability of the keratinocyte system.
[0043]After the samples were filtered and sterilized before and after fermentation, they were added to the culture medium of human keratinocytes (final concentration: 0.05%), and deionized water without sample was used as a blank control. After 48 hours of culture, the cells were stained with MTT method, and the absorbance at 550 nm was measured with a microplate reader. The proliferat...
Embodiment 3
[0048] Example 3 Human keratinocyte scratch test
[0049] Scratch test is the most commonly used model to detect wound healing in vitro, which effectively simulates the process of cell expansion, proliferation and migration covering the wound surface in the early stage of wound healing. Therefore, this model was selected to observe the effect of ginseng flower before and after fermentation on the skin scratch repair ability. Human primary keratinocytes were seeded in 6-well plates, and the cells were confluent to confluence and then scratched, and the samples before and after fermentation (0.05%) were added or not added at the same time. After 24 hours, the cells were fixed and stained with Giemsa staining solution. Observe, take pictures and record the healing of the scratches, taking the 0h scratch as a control, the results are as follows figure 1 shown.
[0050] Depend on figure 1 The results showed that the human primary keratinocytes were treated with ginseng flower fe...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More