Pseudomonadaceae petroleum degrading bacterium L-1, microbial mixed agent of Pseudomonadaceae petroleum degrading bacterium L-1 and in-situ remediation method of petroleum-contaminated soil

A technology of Pseudomonas and mixed bacterial agents, which is applied in the fields of energy biotechnology and environmental biology, can solve the problems of secondary pollution, unsuitable use, and low degree of comprehensive utilization, so as to restore ecological functions and eliminate harm to the human body Effect

Inactive Publication Date: 2019-03-19
BC P INC CHINA NAT PETROLEUM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In practical application, both physical restoration and chemical restoration have certain limitations. In some cases, they are not suitable for use and have proble

Method used

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  • Pseudomonadaceae petroleum degrading bacterium L-1, microbial mixed agent of Pseudomonadaceae petroleum degrading bacterium L-1 and in-situ remediation method of petroleum-contaminated soil
  • Pseudomonadaceae petroleum degrading bacterium L-1, microbial mixed agent of Pseudomonadaceae petroleum degrading bacterium L-1 and in-situ remediation method of petroleum-contaminated soil
  • Pseudomonadaceae petroleum degrading bacterium L-1, microbial mixed agent of Pseudomonadaceae petroleum degrading bacterium L-1 and in-situ remediation method of petroleum-contaminated soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Isolation and Identification of Pseudomonas Pseudomonadaceae Strains

[0034] The present invention provides a Pseudomonas oleaginous bacteria L-1, which is isolated from the oily mud sand of Changqing Oilfield, and is initially identified as belonging to the Pseudomonas genus ( Pseudomonadaceae). The strain can use petroleum hydrocarbons as the sole carbon source for growth, and the simulation test under the conditions of temperature 25-35°C, pH between 7-8, and TDS between 0-800ppm shows that the Pseudomonas can significantly remove petroleum hydrocarbons , The removal rate can reach 77%.

[0035] The oil-contaminated soil suspension was inoculated in the nutrient solution NS-1 with crude oil (6g / L) as the only carbon source at an inoculation amount of 15% by mass, and cultured on a shaker at 150r / min at 28°C for 3-4d under aerobic conditions. , transfer once under the same conditions. Dilute and apply to a solid medium with crude oil as the only carbon s...

Embodiment 2

[0042] The preparation of embodiment 2 microbial mixed bacterial agents

[0043]1. Inoculation and activation of microbial strains

[0044] Under sterile conditions, mix Pseudomonas oleuropeaceae L-1 with indigenous bacteria, [indigenous bacteria are Bacillus encimensis strain encimensis, Alcaligenes faecalisstrain Nocardioid spp. (Nocardioides luteus) Acinetobacter sp) Penicillium oxalicum (Penicillium oxalicum)] according to the ratio of 1:1:1:1:1:1 were inoculated into the Erlenmeyer flask containing nutrient broth (after sterilization ), respectively put the above Erlenmeyer flasks into a shaker at 30°C and 200r / min to incubate for 48h, and set aside.

[0045] 2. Fermentation preparation of liquid mixed bacterial agent

[0046] 2.1 Preparation of bacterial seed solution

[0047] The above-mentioned Pseudomonas oleaginus L-1 and native bacteria cultured for 48 hours were respectively inserted into a bacterial fermentation tank according to the incorporation amount of 5%,...

Embodiment 3

[0050] Example 3 Microbial remediation treatment of oily sludge

[0051] The invention also provides an in-situ remediation method for petroleum-contaminated soil using a microbial mixed bacterial agent. The Pseudomonas oleobacterium L-1 of the present invention can be combined with the enriched and cultivated indigenous bacteria in oil-contaminated soil for in-situ restoration, and according to the crude oil content of the polluted soil, it generally accounts for 20 wt% of the bacterial agent content after mixing. In-situ remediation methods for oil-contaminated soil, including:

[0052] (1) Revive the required microbial strains first, then expand the culture, ferment and produce, and reserve; put them in a fermenter according to a certain ratio at 30°C, and ferment at 200r / min for standby;

[0053] (2) Add native bacteria and bacterial agent nutrient solution according to the corresponding proportion, so that C:N:P=100:10:3 in the soil; carry out ventilation and watering ev...

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Abstract

The invention provides a Pseudomonadaceae petroleum degrading bacterium L-1, a microbial mixed agent of the Pseudomonadaceae petroleum degrading bacterium L-1 and an in-situ remediation method of petroleum-contaminated soil. The preservation number of the Pseudomonadaceae petroleum degrading bacterium is CGMCC NO.16557. The microbial mixed agent contains the Pseudomonadaceae petroleum degrading bacteria in the preservation number being CGMCC NO.16557 and indigenous bacteria. By the aid of the method, the content of petroleum hydrocarbon and polycyclic aromatic hydrocarbon in petroleum-containing sludge can be reduced effectively, harmless treatment of the petroleum-containing sludge can be realized, harms caused by the contaminated soil to human bodies can be eliminated, and ecological functions of the soil can be restored. Thus, degradation of the petroleum hydrocarbon is used as a starting point, microbes having the obvious degrading effect on soil petroleum are separated and cultivated, and the specific implementation measure of an on-site petroleum-contaminated soil microbial in-situ remediation technology is further provided. The petroleum-contaminated soil with the concentration being 3% can be degraded by 77% after 30 days.

Description

technical field [0001] The invention provides a kind of Pseudomonas oleaginus L-1 and its microbial mixed bacterial agent and an in-situ repair method for oil-contaminated soil, belonging to the fields of energy biotechnology and environmental biotechnology. The strain can effectively degrade oil in soil and oil sludge Hydrocarbon substances can be effectively applied to the biodegradation treatment of oil-contaminated soil. Background technique [0002] With the development of the petroleum industry, the pollution of petroleum to the environment is becoming more and more serious. In particular, the corrosion and leakage of underground oil tanks and oil pipelines pollute the soil and groundwater sources, producing a large amount of oily sludge and oily sewage, causing soil salinization, poisoning, etc., and eventually causing great damage to the human body through the accumulation of the food chain. [0003] At present, the treatment methods of oily sludge mainly include ph...

Claims

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Application Information

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IPC IPC(8): B09C1/10
CPCB09C1/10B09C2101/00
Inventor 杨博丽张冕徐迎新王祖文兰建平高银锁谭爱兵李泽锋米志学席仲琛谢涛陈文博姚兰刘小会张晓庆
Owner BC P INC CHINA NAT PETROLEUM CORP
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