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The method and application of preparing high ester bond humic acid preparation by Clostridium beijerinckii

A technology for Clostridium beijerinckii and Clostridium beijerinckii-containing, applied in the directions of microorganism-based methods, biochemical equipment and methods, applications, etc., can solve the problems that the time and location of product release cannot be guaranteed to be controllable and unreachable, etc. To achieve the effect of whole intestinal bacteriostasis and energy supply, promote smooth progress and save separation cost

Active Publication Date: 2022-05-17
QILU UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the deficiencies in the prior art (such as the fermentation process of Clostridium beijerinckii will be inhibited by the product butyric acid; butyric acid will be absorbed by the upper part of the intestinal tract after entering the intestinal tract, resulting in the inability to reach the rear part of the intestinal tract to play a role ; the traditional coating process can not guarantee the controllable time and position of product release in the intestinal tract) and provides a method by adding sodium humate during the fermentation process of Clostridium beijerinckii, under the action of lipase, the butyric acid and humic acid are produced Esterification removes butyric acid from the fermentation broth, and simultaneously generates high-ester bond humic acid to ensure the slow release of butyric acid in the intestinal tract, realizing the antibacterial and energy supply effects of butyric acid in the whole intestine

Method used

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  • The method and application of preparing high ester bond humic acid preparation by Clostridium beijerinckii
  • The method and application of preparing high ester bond humic acid preparation by Clostridium beijerinckii
  • The method and application of preparing high ester bond humic acid preparation by Clostridium beijerinckii

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: a kind of preparation method of high ester bond humic acid preparation, it comprises the steps:

[0026] The first step is to make a seed solution containing Clostridium bejejerinckii, in which the concentration of the bacteria solution is 0.5×10 8 -1.0×10 8 CFU / ml; Clostridium beijerinckii is the preservation number CCTCC No. M2014384 Clostridium beijerinjecki SE-2.

[0027] Seed medium (TYA medium): glucose 40 g, beef extract 2 g, yeast powder 2 g, peptone 6 g, CH 3 COONH 4 3 g, KH 2 PO 4 0.5 g, MgSO 4 ·7H 2 O 0.2 g, FeSO 4 ·7H 2 O 0.01 g, distilled water 1000 mL, pH 6.5, autoclave at 121 ℃ for 15 min, blow with sterile nitrogen for 5 min to drive out oxygen.

[0028] The second step is to prepare butyric acid production fermentation medium, specifically: basic fermentation medium: K 2 HPO 4 0.5 g, KH 2 PO 4 0.5 g, CH 3 COONH 4 2.2 g, MgSO 4 ·7H 2 O 0.2 g, MnSO 4 ·H 2 O 0.01 g, NaCl 0.01 g, FeSO 4 ·7H 2 O 0.01 g, thiamine (thiamine...

Embodiment 2

[0035] Embodiment 2: a kind of preparation method of high ester bond humic acid preparation, it comprises the steps:

[0036] The first step, second step, with embodiment 1.

[0037] In the third step, sodium humate was added to the fermentation medium at a rate of 7 g / L.

[0038] In the fourth step, the sterilization of the fermentation medium in the fermenter is completed, and after cooling down to room temperature, lipase is added to the fermenter at a mass ratio of lipase to sodium humate of 1:3.

[0039] In the fifth step, the Clostridium beijerinckii seed solution and the fermentation medium in the first step are added to the fermentation medium at a volume ratio of 2% by a peristaltic pump, and cultured with stirring at 40°C and 80 rpm.

[0040] In the sixth step, 200g / L xylose is added into the fermenter using a peristaltic pump to maintain the concentration of xylose in the fermentation broth at 20g / L.

[0041] Step 7: At the end of the 96th hour of fermentation, th...

Embodiment 3

[0043] Embodiment 3: a kind of preparation method of high ester bond humic acid preparation, it comprises the steps:

[0044] The first step, second step, with embodiment 1.

[0045] In the third step, sodium humate was added to the fermentation medium at a rate of 10 g / L.

[0046] In the fourth step, the fermentation medium in the fermenter is sterilized, and after cooling down to room temperature, lipase is added to the fermenter at a mass ratio of lipase to sodium humate of 1:1.

[0047] In the fifth step, the Clostridium beijerinckii seed solution and the fermentation medium in the first step are added to the fermentation medium at a volume ratio of 2% using a peristaltic pump, and cultured at 42°C and 100 rpm with stirring.

[0048] In the sixth step, 200g / L xylose is added into the fermenter using a peristaltic pump to maintain the concentration of xylose in the fermentation broth at 20g / L.

[0049] Step 7: At the end of the 96th hour of fermentation, the fermentation ...

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Abstract

A method and application of using Clostridium bejejejerinckii to prepare high-ester bond humic acid preparations, which is to add sodium humate to the fermentation system of Clostridium bejejejerinckii, and use lipase to ferment the butyric acid and humic acid formed by Clostridium bejejejerinckii The phenolic hydroxyl group in sodium undergoes an esterification reaction, which reduces the concentration of butyric acid in the fermentation broth and reduces the inhibitory effect of butyric acid accumulation on the fermentation process of Clostridium beijerinckii. The presence of sodium humate prolongs the fermentation time and product accumulation of Clostridium beijerinckii. The high-ester bond humic acid formed by sodium humate and butyric acid can be directly used as an intestinal health additive product. After entering the intestinal tract, humic acid and butyric acid are released under the action of pancreatic lipase to promote intestinal health.

Description

technical field [0001] The invention relates to the preparation and application of high ester bond humic acid preparation by Clostridium beijerinckii. Background technique [0002] During the fermentation process of Clostridium beijerinckii, the carbon source is converted into butyric acid. With the accumulation of butyric acid, it will inhibit the fermentation process and lead to the end of fermentation. If the butyric acid produced can be removed in time during the fermentation process, the stability of the fermentation process can be ensured and the efficiency of the fermentation process can be improved. [0003] Butyric acid, as a kind of volatile fatty acid, plays an important role in the intestinal health of livestock and poultry. On the one hand, butyric acid can provide energy for intestinal villi, promote the development of intestinal villi, and at the same time inhibit the growth of harmful intestinal bacteria and maintain intestinal tract health. Microecological ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/52C12P7/42C12P7/62A23K50/75A23K20/158C12R1/145
CPCA23K20/158A23K50/70C12P7/42C12P7/52C12P7/62
Inventor 张杰朱小玲丛晓燕王黎文位宾王腾飞
Owner QILU UNIV OF TECH
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