D-amino acid oxidase mutant and application thereof

An amino acid and oxidase technology, applied in the direction of oxidoreductase, application, enzyme, etc., can solve the problems of waste of raw materials, low single resolution rate, complicated process, etc., and achieve high yield, good application value, and tolerance high effect
CN109576236AActive Publication Date: 2019-04-05ZHEJIANG UNIV OF TECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
ZHEJIANG UNIV OF TECH
Publication Date
2019-04-05

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Abstract

The invention discloses a D-amino acid oxidase mutant and application thereof. The mutant is obtained by carrying out single mutagenesis or multiple mutagenesis on a 52nd site, a 54th site, a 58th site, a 213rd site and a 335th site of amino acid with an amino acid sequence shown in SEQ ID NO.1, wherein glycine at the 52nd site is mutated into leucine, asparagine at the 54th site is mutated into valine, phenylalanine at the 58th site is mutated into glutamine, methionine at the 213rd site is mutated into serine, and serine at the 335th site is mutated into glycine. According to the D-amino acid oxidase mutant and the application thereof, a D-amino acid oxidase gene with an amino acid sequence as shown in SEQ ID NO.2 is mutated by utilizing a site-saturation mutagenesis technology, so thatthe enzyme activity and the product conversion rate are far higher than those of a wild type, and therefore the product yield in a 4-(hydroxymethylphosphoryl)-2-carbonyl-butanoic acid production process is increased.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a D-amino acid oxidase mutant and its application. Background technique

[0002] Glufosinate-ammonium, also known as glufosinate, the English name is Phosphinothricin (abbreviated as PPT), the chemical name is 2-amino-4-[hydroxy (methyl) phosphono] butyric acid, it is the second largest transgenic crop resistant to weeding in the world Agent, developed and produced by Hearst (now owned by Bayer after several mergers). Glufosinate-ammonium is a phosphonic acid herbicide, a glutamine synthetase inhibitor, and a non-selective (killing) contact herbicide.

[0003] As we all know, the total herbicide market is huge. At present, the world's three major herbicides are paraquat, glyphosate, and glufosinate-ammonium. In terms of market use, glyphosate is the champion, but due to its long-term use, a large number of weeds have developed resistance, and glyphosate tends to be ineffective; pa...

Claims

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