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vitamin b 2 Modified iron-based nanozyme and its preparation method and application

A vitamin and nano-enzyme technology, which is applied to medical preparations with non-active ingredients, medical preparations containing active ingredients, pharmaceutical formulas, etc., can solve the problems such as the absence of modified iron-based nano-enzymes, and achieve the reduction of ROS scavenging ability, Good biocompatibility and easy preparation

Active Publication Date: 2022-05-17
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, in the prior art, no vitamin B 2 Related reports on modification of iron-based nanozymes
Iron-based nanozymes and vitamin B 2 Combined, which activities can be shown, and whether it has an impact on the activity of iron-based nanozymes, no relevant conclusions have been given in the prior art

Method used

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  • vitamin b <sub>2</sub> Modified iron-based nanozyme and its preparation method and application
  • vitamin b <sub>2</sub> Modified iron-based nanozyme and its preparation method and application
  • vitamin b <sub>2</sub> Modified iron-based nanozyme and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1 Vitamin B 2 Preparation method of modified iron-based nanozyme

[0052] Dissolve 1.35 g of ferric chloride hexahydrate (0.005 mol) in 40 ml of ethylene glycol, and stir with a magnetic stirrer for 30 minutes to fully dissolve ferric chloride hexahydrate to obtain a ferric chloride solution. Add 3.6 g of anhydrous sodium acetate to the above ferric chloride solution, and stir with a magnetic stirrer for 30 minutes to fully dissolve the anhydrous sodium acetate to prepare a mixed solution. Add 0.5 g of vitamin B to the above mixed solution 2 (0.0013mol), stirred for 30 minutes to make vitamin B 2 Mix well to obtain a reaction solution. Then pour all the reaction solution into the reaction kettle, put the reaction kettle into the oven, then raise the temperature of the oven to 200°C, keep warm, heat for 12 hours, wash with alcohol three times, and dry to obtain vitamin B 2 Modified iron-based nanozyme 0.3g, yield 33.9%.

[0053] In this description, other vi...

Embodiment 2

[0055] Embodiment 2 vitamin B 2 Structural characterization of modified iron-based nanozymes

[0056] Vitamin B 2 The structural characterization of the modified iron-based nanozyme is as follows: Figure 1-5 As shown, among them, Figure 1-5 Respectively add-on in embodiment 1 is 0.5g vitamin B 2 The obtained VB 2 -Scanning Electron Microscope, High Resolution Transmission Electron Microscope, Elemental Analysis, X-ray Photoelectron Spectroscopy and X-ray Diffraction of IONzyme.

[0057] Depend on figure 1 and figure 2 Available, adopt the above-mentioned method to prepare the vitamin B that obtains 2 The modified iron-based nanozyme is irregular spherical, the particle size is about 200nm, and the particle size is in the nanoscale range, indicating that the obtained vitamin B 2 Modification of iron-based nanozymes into nanostructures.

[0058] Depend on image 3 It can be obtained that in addition to the peaks of Fe and O elements, there are also peaks of C and N ...

Embodiment 3

[0062] Example 3 Vitamin B 2 Catalase Activity Detection of Modified Iron-Based Nanozymes

[0063] Vitamin B 2 The detection method of the catalase activity of modification iron-based nanozyme, comprises the steps:

[0064] In the sodium acetate solution system with pH=7.0, different concentrations of H 2 o 2 solution, and then add the same concentration of vitamin B 2 Modified iron-based nanozyme solution, or an equal concentration of unmodified iron-based nanozyme solution, real-time monitoring of dissolved oxygen generated in the system, and fitting to obtain H 2 o 2 Concentration-dissolved oxygen generation rate curve. See the results Figure 6 .

[0065] Such as Figure 6 As shown, under neutral conditions, VB 2 - Both IONzyme and IONzyme can decompose hydrogen peroxide and have catalase activity, but VB 2 - The decomposition rate of IONzyme at each concentration is much higher than that of IONzyme, indicating that VB 2 - IONzyme has higher hydrogen peroxide a...

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Abstract

The invention provides a vitamin B 2 Modified iron-based nanozymes, specifically vitamin B 2 Modified nano-scale ferric oxide particles, also provides vitamin B 2 A preparation method of a modified iron-based nanozyme and a composition for promoting the healing of oral ulcers. Vitamin B provided by the present invention 2 Modified iron-based nanozyme, which has higher catalase activity and superoxide dismutase activity than iron-based nanozyme, and compared with vitamin B used alone 2 and iron-based nanozymes, vitamin B provided by the invention 2 The modified iron-based nanozyme showed a better protective effect on BALB / 3T3 cells and HOK cells in hydrogen peroxide environment, ROS scavenging ability at the cellular level, and the ability to reduce IFN-γ and IL-6 in ulcers. It has better therapeutic effect and can be used to prepare a medicine for promoting the healing of oral ulcer with high efficiency.

Description

technical field [0001] This application relates to the technical field of biological nanomaterials, in particular to a vitamin B 2 Modified iron-based nanozyme and its preparation method and application. Background technique [0002] Iron-based nanozyme (IONzyme) is a class of mimetic enzymes that have both the unique properties of nanomaterials and catalytic functions. At present, experiments have found that the iron-based nanozyme has both catalase (CAT) activity and superoxide dismutase (SOD) activity, and has a series of advantages such as simple preparation, low cost, stability and easy storage. [0003] Oral ulcer, commonly known as "oral sore", is a common ulcerative injury disease that occurs in the oral mucosa. It is more common in the inner lip, tongue, tongue belly, buccal mucosa, vestibular groove, soft palate and other parts. The mucosa in these parts lacks stratum corneum. or poor keratosis. Tongue ulcers refer to oral ulcers that occur on the tongue and the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/55A61K33/26A61K31/525A61P17/02A61P1/02
CPCA61K47/551A61P1/02A61P17/02A61K31/525A61K33/26A61K2300/00
Inventor 高利增顾云浩仇智月李丹丹
Owner YANGZHOU UNIV
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