A kind of xylanase xylanase-m with high thermostability and its coding gene and application

A xylan and gene technology, applied in the field of genetic engineering, can solve the problems of large loss of activity and increased cost, and achieve the effects of improved thermal stability, improved practicability, and significant application value
CN109652393BActive Publication Date: 2020-10-09INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
Publication Date
2020-10-09

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Abstract

The invention discloses xylanase-m with high heat stability and its coding gene and an application thereof. The protein provided by the present invention is named as xylanase-m, also known as xylanase-m protein, which is as follows (a1), (a2) or (a3): (a1) a protein consisting of 11st to 235th amino acid residues in SEQ ID NO: 1 of a sequence table; (a2) a protein represented by SEQ ID NO: 1 of the sequence table; and (a3) a fusion protein obtained by connecting a label at the N-terminus or / and C-terminus of (a1). The invention also provides an application of xylanase-m protein as xylanase. The invention also provides an application of xylanase-m protein in degrading xylan. The invention also provides an application of the xylanase-m protein in reducing sugar production by taking xylan asa substrate. The xylanase-m can be used in the food industry, animal feed industry and paper bleaching industry, and has great application value.
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Description

Technical field

[0001] The invention belongs to the field of genetic engineering, and specifically relates to a xylanase xylanase-m with high thermostability and its coding gene and application. Background technique

[0002] Hemicellulose is the second most abundant component in plant cell walls and the second most abundant polysaccharide in nature. Xylan is the most important component of hemicellulose, accounting for about 15-30% of the dry weight of angiosperm cells. However, hemicellulose or xylan is difficult to degrade. The complete degradation of xylan requires multiple enzymatic reactions and The synergistic effect of multiple hydrolases, of which β-1,4-endo-xylanase (endo-1,4-β-xylanase, EC3.2.1.8) and β-xylanase are the most critical hydrolases. Xylanase can open the xylan bonds of xylan and effectively degrade high xylan into xylose monomer or oligoxylan.

[0003] In papermaking, xylanase treatment of pulp can effectively release lignin, so that pigments can be separat...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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