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Preparation method and application of Alpinia japonica spice for tobaccos

A technology for tobacco flavors and ginger, applied in the application, essential oil/spice, tobacco and other directions, can solve the problems of insufficient preparation methods, insufficient types of tobacco flavors and spices, and reduce the irritation of smoke, spices and processing. The process is environmentally friendly and safe, and the effect of enhancing the fragrance effect

Inactive Publication Date: 2019-05-07
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a method for preparing a spice for tobacco with spicy, fruity and herbal flavors and its use in cigarettes to solve the problems that the types of flavors and fragrances for tobacco are not rich enough and the preparation method is insufficient. application, which uses the X7X fermentation process of tobacco yellow soil-derived bacteria to process alpinia officinalis, and then obtains an alpinia officinalis tobacco spice through a solvent extraction process, which is applied to shredded cigarette tobacco, so that the smoke has special spicy, fruity and Herbal aroma, fresh and soft, can enrich the cigarette aroma, reduce the irritation of smoke, enhance the flavoring effect of cigarettes, and enrich the sources and types of flavors and fragrances for tobacco

Method used

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  • Preparation method and application of Alpinia japonica spice for tobaccos
  • Preparation method and application of Alpinia japonica spice for tobaccos
  • Preparation method and application of Alpinia japonica spice for tobaccos

Examples

Experimental program
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Effect test

Embodiment 1

[0036] 1. Isolation, cultivation and identification of tobacco yellow soil-derived bacteria X7X

[0037] 1.1 Isolation of tobacco yellow soil-derived bacteria X7X

[0038]Soil samples were taken from the tobacco rhizosphere of the tobacco production base in Kunming, Yunnan Province, sealed in plastic bags, and stored at 4°C for later use. Accurately weigh 10g of soil sample, add 100mL of sterile water, shake and incubate at 30°C, 200rpm for 30min; dilute the concentration to 10 with sterile water -3 、10 -4 W / v times, take 0.1mL of the two concentrations of bacteria solution and evenly spread it on the R2A agar plate, set up three parallel experiments for each concentration of the bacteria solution, and cultivate it in a constant temperature incubator at 30°C for 2 weeks. Pick single colonies of different shapes from above, continue to use plate streaking method to isolate and purify the strains, inoculate them on R2A slant medium after numbering, or prepare glycerol suspensi...

Embodiment 2

[0056] 1. Cultivation of Tobacco Yellow Soil Bacteria X7X

[0057] (1) Test tube slant culture

[0058] Slope preservation medium is used, the medium is R2A agar medium, and the formula of the medium is: 0.5g of glucose, 0.5g of yeast extract, 0.5g of peptone, 0.5g of acid hydrolyzed casein, 0.5g of soluble starch, and 0.3g of sodium pyruvate , dipotassium hydrogen phosphate 0.3g, magnesium sulfate 0.05g, agar 15g, distilled water to 1000mL, pH7.2. Sterilize the culture medium at 121°C for 25 minutes, place it on a slant, inoculate tobacco yellow soil-derived bacteria X7X, and cultivate it at 30°C for 2 weeks to obtain the test tube strain;

[0059] (2) Seed cultivation

[0060] The seed medium is R2A liquid medium, and the formula is: glucose 0.5g; yeast extract 0.5g; peptone 0.5g; acid hydrolyzed casein 0.5g; soluble starch 0.5g; sodium pyruvate 0.3g; hydrogen phosphate Dipotassium 0.3g; magnesium sulfate 0.05g; distilled water 1000mL, pH7.2. Sterilize the medium at 121°...

Embodiment 3

[0078] 1. The cultivation method of tobacco yellow soil-derived bacterium X7X is the same as embodiment 2.

[0079] 2. Ginger slurry fermentation

[0080] Add 1kg of fresh Ginger rhizomes to 1 times the quality of water, use a beater for preliminary crushing, adjust the distance between the colloidal grinding and static grinding gears to 0.3 mm, and grind the Ginger Ginger coarse pulp twice with a colloid mill to obtain Ginger Ginger slurry. Spray tobacco yellow soil-derived bacteria X7X inoculum 20mL in every 100g of Ginger slurry, mix, place 22 ℃ of processed Ginger slurry, ferment 36h in 60% constant temperature and humidity box, obtain Ginger fermentation liquid.

[0081] 3. Preparation of the concentrated extract of Alpinia officinalis

[0082] After sterilizing the obtained alpinia officinale fermentation liquid in a sterilizing pot at 121°C for 30 minutes, add distilled water with 5 times the mass of the alpinia officinalis liquid, heat it to a slight boiling state with...

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Abstract

The invention provides a preparation method and application of an Alpinia japonica spice for tobaccos, and the preparation method comprises the following steps: (1) preparing a tobacco yellow soil source microbial agent X7X; (2) fermenting Alpinia japonica powder by using the tobacco yellow soil source microbial agent X7X to obtain Alpinia japonica fermentation broth; (3) heating, refluxing and extracting the Alpinia japonica fermentation broth by using distilled water, and filtering and concentrating to obtain an Alpinia japonica concentrated extracting solution; (4) redissolving the Alpiniajaponica concentrated extracting solution by using ethanol, carrying out uniform stirring, freezing and settling, and concentrating supernate to obtain the Alpinia japonica spice for tobaccos. The Alpinia japonica spice for tobaccos is applied to cigarette tobacco shreds, so that smoke has special spicy, fruity and herbal fragrance, the fragrance is fresh and soft, the fragrance of cigarettes canbe enriched, smoke irritation of the cigarettes can be reduced, the fragrance-forming effect of the cigarettes can be enhanced, and the smoking quality of the cigarettes can be improved.

Description

technical field [0001] The invention belongs to the technical field of flavors and fragrances for tobacco, and in particular relates to a preparation method and application of Alpine ginger tobacco flavors. Background technique [0002] Cigarette aroma is the core content of cigarette quality, but with the development of low-coking cigarettes, the reduced taste of cigarette smoking and insufficient aroma have become prominent problems in the development of tobacco. Cigarette flavoring and addition is already a routine and necessary technical means to supplement the aroma. By means of flavoring and adding ingredients, it can make up for the deficiency in the formula of the cigarette leaf group, eliminate miscellaneous gases, and make the cigarette aroma richer and more sufficient. Therefore, it has a unique style in absorbing and smelling fragrance, and enhances the characteristics of the product. Natural flavors play an extremely important role in cigarette flavoring, and t...

Claims

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Application Information

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IPC IPC(8): C11B9/00A24B15/30
Inventor 刘秀明段焰青杨登强李文均张翼鹏高莉王凯
Owner CHINA TOBACCO YUNNAN IND
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