Method for constructing nanometer-scale or micrometer-scale complex condensates by utilizing rice protein and Arabic gum
A gum arabic and rice protein technology, applied in the field of food processing, can solve the problems of low solubility of rice protein, limited application fields, and inability to form a viscoelastic gel network structure, etc., and achieve the effect of simple method, mild conditions and low cost
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Embodiment 1
[0028] After the broken rice was ground, it was stirred and extracted with 70% ethanol for 1h (material-to-liquid ratio 1:5, g / mL), filtered and dried naturally for 24h, crushed through an 80-mesh sieve and stirred with distilled water to extract albumin for 1h (material-to-liquid ratio 1:5) 10, g / mL), after centrifugation (3000g, 30min), the precipitation was stirred with 5% NaCl to extract globulin for 1h (material-to-liquid ratio 1:10, g / mL), after centrifugation (3000g, 30min), the precipitation was washed with 0.05mol / L NaOH stirring and extracting for 1h (material-to-liquid ratio 1:10, g / mL), centrifuge (3000g, 30min), adjust the pH of the supernatant to 4.8 with 12% trichloroacetic acid, centrifuge (3000g, 30min), for precipitation Disperse in distilled water, adjust the pH to 7.0, dialyze overnight in an 8000Da dialysis bag and freeze-dry. The protein extraction rate was 56.40±2.20%, the moisture content was 3.88±0.22%, and the protein content was 96.82±0.13% (dry basi...
Embodiment 2
[0030] After the broken rice was ground, it was stirred and extracted with 70% ethanol for 2h (material-to-liquid ratio 1:3, g / mL), filtered and dried naturally for 24h, crushed through an 80-mesh sieve, and distilled water was stirred to extract albumin for 2h (material-to-liquid ratio 1:3) 5, g / mL), after centrifugation (3500g, 15min), the precipitate was stirred with 5% NaCl to extract globulin for 2h (material-to-liquid ratio 1:5, g / mL), after centrifugation (3500g, 15min), the precipitate was washed with 0.05mol / LNaOH, stirring and extracting for 2h (material-to-liquid ratio 1:5, g / mL), centrifuge (3500g, 15min), adjust the pH of the supernatant to 4.8 with 12% trichloroacetic acid, centrifuge (3500g, 15min), use distilled water for precipitation Disperse, adjust the pH to 7.0, dialyze overnight in a 14000Da dialysis bag and freeze-dry. The protein extraction rate was 56.40±2.20%, the moisture content was 3.88±0.22%, and the protein content was 96.82±0.13% (dry basis). W...
Embodiment 3
[0032] Titrate 1.0% rice protein solution to 5mL 0.1% gum arabic solution to make the protein-polysaccharide mass ratio 0.1. After mutual titration, place the reaction system in a constant temperature water bath stirred reactor at 40°C and mix immediately. 1% (w / v) rapeseed oil was added to the mixture, dispersed by a high-speed disperser at a certain speed for 2 minutes, and then samples were taken at 0 and 10 minutes respectively, using the pre-prepared 1 mg / mL sodium lauryl sulfate ( The SDS) solution was diluted 10 times, and the sodium dodecyl sulfate solution was used as a blank control, and the absorbance was measured at a wavelength of 500 nm. Use A respectively 0 And A 10 It represents the absorbance value of the system at 0 min and 10 min. Use the emulsifying activity index (EAI, m 2 / g) and emulsion stability index (ESI, min) to indicate the emulsification activity and emulsion stability of the system, the results are shown in Table 1. Under the conditions of the pr...
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