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A rapid detection method for dithiocarbamate pesticides

A technology of dithiocarbamate and detection method, applied in material excitation analysis, Raman scattering, etc., can solve the problems of high cost, slow result, unfavorable rapid detection, etc.

Active Publication Date: 2021-04-30
苏州英菲尼纳米科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this patent solves the problem of false positives in the colorimetric method to a certain extent, the actual operation is still cumbersome, the detection speed is slow, and the cost is high, which is not conducive to the detection of dithiocarbamate pesticides contained in fruits and vegetables. Perform a quick test

Method used

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  • A rapid detection method for dithiocarbamate pesticides
  • A rapid detection method for dithiocarbamate pesticides
  • A rapid detection method for dithiocarbamate pesticides

Examples

Experimental program
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Effect test

preparation example Construction

[0060] Preparation of nano-silver sol:

[0061] Add 1mL of 1% silver nitrate aqueous solution to a 100mL three-necked flask and dilute to 100ml; heat the solution to boiling, and add 1% sodium citrate solution under constant reflux and vigorous stirring. The solution gradually changed from colorless to light blue. After the discoloration, start timing, keep the system boiling for 15 min with stirring, and then naturally cool to room temperature to obtain silver sol.

[0062] Preparation of nano-gold sol:

[0063] Add 1mL of 1% chloroauric acid aqueous solution into a 100mL three-necked flask and dilute to 100ml; heat the solution to boiling, and add 1% sodium citrate solution under constant reflux and vigorous stirring. The solution gradually turned from light yellow to wine red. Start timing after discoloration, keep the system boiling for 15 minutes under stirring conditions, and then naturally cool to room temperature to obtain gold sol. Adjust the amount of sodium citra...

Embodiment 1

[0070] A method for the detection of dithiocarbamate pesticides in agricultural products proposed in this example comprises the following steps:

[0071] 1. Weigh 10g of red dragon fruit crushed (thiram, 0.5mg / L), add 10ml of acetonitrile solution, add 2.5g of sodium chloride solid, shake for 2min, add 5g of anhydrous sodium sulfate to fully shake the sample for 3min, centrifuge at 5000rpm for 5min ;

[0072] 2. Take the upper layer solution (that is, the acetonitrile solvent phase) in a test tube and dry it with nitrogen;

[0073] 3. Take 200 μL of methanol in the above test tube and shake it several times as the test solution;

[0074] 4. Soak the SERS substrate I in the above 150 μL test solution for 10 minutes, take it out, wash it with ethanol, dry it, and test it with a Raman spectrometer. get as figure 2 surface-enhanced Raman spectroscopy.

Embodiment 2

[0076] A method for the detection of dithiocarbamate pesticides in agricultural products proposed in this example comprises the following steps:

[0077] 1. Weigh 10g of crushed banana (Dysenlian, 1mg / L), add 10ml of acetonitrile solution, add 2.0g of potassium chloride solid, shake for 2 minutes, add 4g of anhydrous magnesium sulfate, shake the sample again for 3 minutes, and centrifuge at 5000rpm for 5 minutes;

[0078] 2. Take 2 mL of the upper layer solution (that is, the acetonitrile solvent phase) and filter it as the solution to be tested;

[0079] 3. Soak the SERS substrate II in the above-mentioned 200 μL test solution for 10 minutes, take it out, wash it with ethanol, dry it, and test it with a Raman spectrometer. get as image 3 surface-enhanced Raman spectroscopy.

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Abstract

The invention discloses a method for detecting dithiocarbamate pesticides in agricultural products, comprising: 1) mixing the agricultural products to be tested with an organic solvent, a first salt bag, and a second salt bag, so that the Pesticide-like pesticides are put into the organic solvent, and after extraction, filtration or centrifugation, a clear liquid test sample is obtained. The first salt bag is used to destroy the cell structure of the agricultural product, and the second salt bag is used to absorb the moisture in the agricultural product; 2) the SERS substrate The active area is immersed in the liquid detection sample for a predetermined time, so that the dithiocarbamate pesticide molecules are adsorbed or grabbed onto the surface of the SERS substrate; 3) the SERS substrate obtained in step (2) is dried and then subjected to Raman detection; the present invention On the premise of high detection accuracy, high detection sensitivity and low detection limit, it can have the advantages of simple operation and low cost.

Description

technical field [0001] The invention belongs to the technical field of pesticide detection, and in particular relates to a rapid detection method for dithiocarbamate pesticides, in particular to the detection of dithiocarbamate pesticides contained in fruits, vegetables and crops. Background technique [0002] Dithiocarbamate pesticides are insecticides widely used in crops. They can interact with enzymes containing sulfhydryl groups (-SH) and coenzymes of fungal cells to achieve the purpose of killing insects. Although the toxicity of this compound is low, the application of this compound in fruits and vegetables still has potential environmental pollution problems and affects human health, such as irritating human skin and mucous membranes, and its derivatives have the possibility of carcinogenicity and human deformity , Therefore, at present, the residual dithiocarbamate pesticides in crops are tested to determine whether they meet national or international standards. ...

Claims

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Application Information

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IPC IPC(8): G01N21/65
Inventor 孙海龙周娜娜郭清华马从乔
Owner 苏州英菲尼纳米科技有限公司
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