Preparation method and application of electrochemical sensor for non-coding RNA

An electrochemical and sensor technology, applied in the field of electrochemical biosensing, can solve the problems of cumbersome sample processing, high detection cost, and high maintenance cost, and achieve the effects of good chemical stability, low detection limit, and time saving.
CN109844514AActive Publication Date: 2019-06-04YUNNAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
YUNNAN UNIV
Publication Date
2019-06-04

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Abstract

A method for preparing an electrochemical sensor for non-coding RNA, which comprises preparing a sulfonated cup [8] arene-supported reduced graphene oxide by a one-step method, synthesizing a ferroferric oxide nano material by a moist heating method, and adding gold nanoparticles to synthesize Au@SCX8-RGO and Au@Fe3O4 composite materials. After confirming successful material construction by material characterization, a target material is electrochemically detected by a screen printing electrode with toluidine blue and other materials used as electrical signal substances. The electrochemical biosensor prepared by the method can detect the expression quantity of 3'UTR transcripts of non-coding regions of different lengths produced by the selective polyadenylation (APA) phenomenon.
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Description

technical field

[0001] The invention belongs to the field of electrochemical biosensing, and in particular relates to an electrochemical detection method of non-coding RNA and its application in selective polyadenylation detection. Background technique

[0002] Ultra-sensitive nucleic acid detection technology is widely used in functional gene detection and its biological function evaluation, disease diagnosis and other fields. Such as fluorescent quantitative PCR technology, rolling circle amplification technology and other trace nucleic acid detection technologies are based on polymerase chain reaction and fluorescent signal detection methods to achieve trace nucleic acid quantification or detection, but these detection methods have complex operation steps, expensive optical signal probes, and require Specific detection equipment and other issues. Electrochemical sensing technology has always been the most widely used biomedical and chemical analysis detection platform, w...

Claims

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