Check patentability & draft patents in minutes with Patsnap Eureka AI!

Nano composite fluorescent probe for visibly detecting quercetin and preparation method thereof

A nanocomposite, fluorescent probe technology for applications in nanotechnology, nanooptics, chemical instruments and methods for materials and surface science

Inactive Publication Date: 2019-06-11
QINGDAO UNIV
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the literature search, although the method of quantifying quercetin based on the intensity of fluorescence has been reported in the literature, there are no domestic and foreign literature and patent reports on the fluorescence visual detection method of quantifying quercetin based on the type of fluorescence color.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Nano composite fluorescent probe for visibly detecting quercetin and preparation method thereof
  • Nano composite fluorescent probe for visibly detecting quercetin and preparation method thereof
  • Nano composite fluorescent probe for visibly detecting quercetin and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The present invention relates to a nanocomposite fluorescent probe for the visual detection of quercetin and its preparation method. The preparation process and principle are as follows: figure 1 As shown, the specific preparation steps are as follows:

[0023] Weigh 1g of sodium citrate and 2g of ammonium carbonate, dissolve them in 30mL of twice-distilled water under magnetic stirring, transfer the mixed solution into a 50mL autoclave with a polytetrafluoroethylene liner, stir at room temperature for 30min, and heat up to 120 ℃ after the reaction for 12h. Cool the product solution to obtain a dark brown suspension, filter through a 0.22 μm filter membrane and collect the filtrate, remove most of the solvent by rotary evaporation, centrifuge the residual filtrate, wash the precipitate with ethanol, and dry the purified carbon dots in vacuum at 60°C Prepare carbon dot powder. Disperse the carbon dot powder in phosphate saline buffer solution, add rhodamine 6G aqueous ...

Embodiment 2

[0025] Weigh 1g of sodium citrate and 4g of ammonium carbonate, dissolve them in 30mL of twice distilled water under magnetic stirring, transfer the mixed solution into a 50mL autoclave with a polytetrafluoroethylene liner, stir at room temperature for 30min, and heat up to 150 ℃ after the reaction for 10h. Cool the product solution to obtain a dark brown suspension, filter through a 0.22 μm filter membrane and collect the filtrate, remove most of the solvent by rotary evaporation, centrifuge the remaining filtrate, wash the precipitate with ethanol, and dry the purified carbon dots in vacuum at 60°C Prepare carbon dot powder. Disperse the carbon dot powder in phosphate saline buffer solution, add rhodamine 6G aqueous solution dropwise under ultrasonic and magnetic stirring, in which the mass concentration of carbon dots is 4mg mL -1, the rhodamine 6G molar concentration was 20 μM, the phosphate concentration was 20 mM, the pH of the buffer solution was 7.4, and the ultrasoni...

Embodiment 3

[0027] Weigh 1g of sodium citrate and 5g of ammonium carbonate, dissolve them in 30mL of double-distilled water under magnetic stirring, transfer the mixed solution into a 50mL autoclave with a polytetrafluoroethylene liner, stir at room temperature for 30min, and heat up to 180 After reaction at ℃ for 8h. Cool the product solution to obtain a dark brown suspension, filter through a 0.22 μm filter membrane and collect the filtrate, remove most of the solvent by rotary evaporation, centrifuge the residual filtrate, wash the precipitate with ethanol, and dry the purified carbon dots in vacuum at 60°C Prepare carbon dot powder. Disperse the carbon dots powder in phosphate saline buffer, add rhodamine 6G aqueous solution dropwise under ultrasonic and magnetic stirring, in which the mass concentration of carbon dots is 5mg mL -1 , the rhodamine 6G molar concentration was 50 μM, the phosphate concentration was 30 mM, the pH of the buffer solution was 8.0, and the mixture was kept u...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of the preparation of nano composites and visual fluorescent probes, and particularly relates to a nano composite fluorescent probe for visually detectingquercetin and a preparation method thereof. The excellent electron-harvesting effect of the quercetin can cause photo-induced electron transfer and quenching of fluorophores under photon excitation. Quercetin can simultaneously cause the quenching of carbon point green fluorescence and rhodamine 6G red fluorescence in electrostatic adsorption self-assembled carbon point / rhodamine 6G nanocomposites. With the increase of the quercetin concentration, the fluorescence color of a composite probe solution in a centrifuge tube and dripping on filter paper gradually changes from deep red, reddish orange, orange, deep yellow, yellow green, light green to deep green. The correlation between different concentrations of quercetin and fluorescence colors of the probe solution was established, and the quercetin is visually detected based on the fluorescence color type. Compared with the prior art, the probe has the advantages of simple preparation, low cost and high sensitivity, and can be used forthe fluorescence visual detection of quercetin in biological samples.

Description

Technical field: [0001] The invention belongs to the technical field of preparation of nanocomposites and visualized fluorescent probes, in particular to a nanocomposite fluorescent probe for visually detecting quercetin and a preparation method thereof, and the prepared probe can be used in quercetin in biological samples Fluorescent visualization of corticosteroids. Background technique: [0002] Quercetin (3,3',4',5,7-quinercetin) is a common flavonol, which widely exists in vegetables, fruits, beverages and other nutritious foods as a natural compound. Among the nutrients obtained through diet, the intake of flavonols by humans is about 2.6-38.2 mg / day. Clinical medicine has confirmed that quercetin is beneficial to human health. It can prevent diabetes, inhibit headache shock, reduce capillary fragility, induce apoptosis, and have the functions of anti-inflammatory drugs. Quercetin has an important impact on maintaining life activities, has specific biomedical effects...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/64C09K11/65C09K11/06C09K11/02B82Y20/00B82Y30/00B82Y40/00
CPCB82Y20/00B82Y30/00B82Y40/00C09K11/02C09K11/06C09K11/65G01N21/64
Inventor 金辉桂日军卜祥宁付永鑫姜晓文
Owner QINGDAO UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com