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Tissue engineering soft repair scaffold with globular protein as pore-foaming agent and preparation method thereof

A globular protein and tissue engineering technology, applied in the direction of prosthesis, medical science, etc., can solve the problems of high cost, limited instrument accuracy, application limitations, etc., to achieve the effect of promoting the formation of cartilage tissue, compact appearance and rapid cartilage damage

Active Publication Date: 2019-06-14
NORTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Rapid prototyping technology can accurately prepare tissue engineering scaffolds according to the preset shape and structure, but in actual operation, because of the limited accuracy of the instrument, and different technologies can only choose corresponding materials, other solvents need to be added during the production process And the problem of high cost makes its application in tissue engineering subject to certain restrictions

Method used

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  • Tissue engineering soft repair scaffold with globular protein as pore-foaming agent and preparation method thereof
  • Tissue engineering soft repair scaffold with globular protein as pore-foaming agent and preparation method thereof
  • Tissue engineering soft repair scaffold with globular protein as pore-foaming agent and preparation method thereof

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preparation example Construction

[0034] The present invention relates to a preparation method of a tissue engineering soft repair scaffold using globular protein as a porogen. Collagen (such as human-like collagen produced by high-density fermentation of genetically engineered bacteria BL21) is used as a raw material, and transglutaminase derived from microorganisms is used as a raw material. The cross-linking agent, globular protein and NaCl are porogens, and the tissue-engineered cartilage scaffold is prepared by biological cross-linking. The steps include: dissolving the collagen in the globular protein solution, and finally preparing the tissue engineered cartilage scaffold by using the pore-forming agent NaCl and the cross-linking agent glutamine transaminase.

[0035] Specifically include the following steps:

[0036] Step (a): dissolving the globular protein in double distilled water to obtain a globular protein solution with a mass fraction of 5%-15%;

[0037] Step (b): dissolving collagen in the glo...

Embodiment 1

[0047] Step (a): Weigh soybean protein and dissolve it in 5 mL double-distilled water to obtain a bovine serum albumin solution with a mass fraction of 5%;

[0048] Step (b): the human-like collagen is dissolved in the bovine serum albumin solution in step (a), so that its concentration is 15%;

[0049]Step (c): Put the protein mixture solution prepared in step (b) at 37°C to dissolve completely, add 4% NaCl, and stir to dissolve completely;

[0050] Step (d): Add transglutaminase with a content of 60 U / g collagen to the mixed solution prepared in step (c), and after completely dissolving, statically cross-link at 4°C for 24 h to obtain a human collagen cartilage scaffold ;

[0051] Step (e): The cartilage scaffold obtained in step (d) was washed in pyrogen-free water for 72 hours to remove the crosslinking agent and porogen, vacuum freeze-dried, and sterilized by Co60 irradiation to obtain a tissue engineered cartilage scaffold.

Embodiment 2

[0053] Step (a): Weigh bovine serum albumin and dissolve it in 5 mL double-distilled water to obtain a bovine serum albumin solution with a mass fraction of 5%;

[0054] Step (b): the human-like collagen is dissolved in the bovine serum albumin solution in step (a), so that its concentration is 15%;

[0055] Step (c): Put the protein mixture solution prepared in step (b) at 37°C to dissolve completely, add 1% NaCl, and stir to dissolve completely;

[0056] Step (d): Add transglutaminase with a content of 80 U / g collagen to the mixed solution prepared in step (c), and after complete dissolution, statically cross-link at 4°C for 24 h to obtain human collagen cartilage scaffolds ;

[0057] Step (e): The cartilage scaffold obtained in step (d) was washed in pyrogen-free water for 72 hours to remove the crosslinking agent and porogen, vacuum freeze-dried, and sterilized by Co60 irradiation to obtain a tissue engineered cartilage scaffold.

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Abstract

The invention relates to a tissue engineering soft repair scaffold with globular protein as a pore-foaming agent and a preparation method thereof. Collagen is dissolved in a globular protein solution,NaCl and glutamine transaminase are added, and finally the tissue engineering articular cartilage scaffold is prepared, wherein the globular protein and NaCl are pore-foaming agents and glutamine transaminase is used as a crosslinking agent. The prepared cartilage scaffold has a three-dimensional porous structure with high penetration property, has the advantages of good biocompatibility, safety,no toxicity, degradability, and appropriate mechanical properties, and is an ideal material for repairing human articular cartilage tissues.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a tissue engineering soft repair support using globular protein as a porogen and a preparation method thereof. Background technique [0002] Articular cartilage is a kind of tissue with few cells, no blood vessels, and no lymph, so its regeneration and self-repair ability is poor, and the damage that does not reach the subchondral bone can hardly heal, and only transient chondrocytes can be produced near the wound to replicate and synthesize A small amount of matrix, and finally gradually cause joint surface degeneration, the formation of arthritis. At present, there are several mature methods for the treatment of cartilage injuries as follows: one is autologous cartilage transplantation, which has a good clinical effect, but cartilage tissue must be cut from healthy parts, resulting in new defects, and the autologous donor area is also very limited; the other is a...

Claims

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Application Information

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IPC IPC(8): A61L27/24A61L27/22A61L27/50A61L27/56A61L27/58
Inventor 朱晨辉范代娣米钰马晓轩宋茜段志广傅容湛李伟娜
Owner NORTHWEST UNIV
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