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Preparation method of biological natural preservative and application thereof in sturgeon caviar

A preservative and natural technology, applied in application, food science, food preservation, etc., can solve the problems of poor water solubility and limited application range, and achieve the effects of improving water solubility, enhancing antibacterial activity, and extending shelf life

Active Publication Date: 2019-06-25
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Octyl gallate (GAC8) has good antibacterial activity and can be used as a natural fresh-keeping agent in the food field, but its poor water solubility limits its application range in the aquatic product field

Method used

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  • Preparation method of biological natural preservative and application thereof in sturgeon caviar
  • Preparation method of biological natural preservative and application thereof in sturgeon caviar
  • Preparation method of biological natural preservative and application thereof in sturgeon caviar

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Experimental program
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Effect test

Embodiment 1

[0029] The steps of preparing GAC8 by the enzymatic method in the deep eutectic solvent are as follows:

[0030] Mix acetylcholine with two hydrogen bond donors, urea and glycerol (mole ratio 1:1:1), stir at 80°C for 2 hours to obtain a transparent liquid which is a deep eutectic solvent, wherein the water content in the deep eutectic solvent 10% (w / w). In a deep eutectic solvent, lipase (Novo 435, 10 mg) was used to catalyze the transesterification reaction of methyl gallate and octanol (molar ratio 1:2-10) to form a crude product of octyl gallate, which was then further purified by chromatography. After purification, the product phase was collected, and the solvent was removed under reduced pressure to obtain the pure product of GAC8 (>98%, HPLC). The resulting esterification yields are shown in figure 1 .

Embodiment 2

[0032] A natural biological preservative in the form of solid powder, the molar ratio of GAC8 and HP-β-CD in the powder is 1:1.5.

[0033] The preparation and application of this biological natural preservative comprise the following steps:

[0034] (1) In deionized water, add HP-β-CD, stir magnetically at room temperature until completely dissolved; then slowly add GAC8 absolute ethanol solution into HP-β-CD aqueous solution, and keep stirring; use a rotary evaporator Remove ethanol; refrigerate overnight at -20°C, freeze-dry (-50°C, 36h) to obtain GAC8 / HP-β-CD powder.

[0035] (2) a processing method for spraying sturgeon caviar with green antistaling agent and prolonging its storage period, comprising the following steps:

[0036] (3) Material selection: Select suitable female sturgeon with gonad development as broodstock to collect eggs, and the egg diameter is above 2-8mm.

[0037] (4) Egg extraction and roe separation: soak the broodstock in ice water at 5-8°C for 30-6...

Embodiment 3

[0045] A biological natural preservative in the form of solid powder, the molar ratio of GAC8 and HP-β-CD in the powder is 1:1.2

[0046] The preparation and application of this biological natural preservative comprise the following steps:

[0047] (1) In deionized water, add HP-β-CD, and stir magnetically at room temperature until it is completely dissolved; then slowly add GAC8 absolute ethanol solution dropwise to the previously prepared HP-β-CD aqueous solution, and continue stirring; Ethanol was removed by a rotary evaporator; GAC8 / HP-β-CD powder was obtained after refrigerated overnight at -20°C and freeze-dried (-50°C, 36h).

[0048](2) a processing method for spraying sturgeon caviar with green antistaling agent and prolonging its storage period, comprising the following steps:

[0049] (3) Material selection: Select suitable female sturgeon with gonad development as broodstock to collect eggs, and the egg diameter is above 2-8 mm.

[0050] (4) Egg extraction and roe...

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Abstract

The invention relates to a preparation method of a biological natural preservative and application thereof in sturgeon caviar. An octyl gallate / 2-hydroxypropyl-beta-cyclodextrin encapsulation naturalpreservative is disclosed. The octyl gallate and 2-hydroxypropyl-beta-cyclodextrin are encapsulated according to a molar ratio of 1:0 .5-1.5. A method for preparing the octyl gallate by an enzymatic method in a deep eutectic solvent is also disclosed. In addition, the application of the preservative in the preservation of the sturgeon caviar is also disclosed. The processed caviar not only maintains the efficacy for a long time, but also effectively reduces the storage cost. The preservative is tasteless, non-toxic and edible, and the components are safe and environmentally friendly. The watersolubility and the antibacterial effect of the octyl gallate are improved, and the pollution of pathogenic bacteria to aquatic products during storage and transportation is effectively inhibited. Theshelf life can be effectively prolonged. The application range of the octyl gallate as the preservative in the food field is expanded.

Description

technical field [0001] The invention relates to a food antiseptic and antistaling agent, in particular to a bacteriostatic agent, which is especially used in the preservation of aquatic products. Background technique [0002] Sturgeon roe sauce (Caviar) is an expensive aquatic product made by screening and salting sturgeon roe, and it is also the main product of the sturgeon processing industry. Sturgeon roe sauce is rich in essential amino acids and high unsaturated fatty acids (EPA, DHA), inorganic salts, vitamins A, B and D, and trace elements such as calcium, copper, magnesium, iron and selenium, not only has health benefits The efficacy also has the potential to be developed into skin care products. China is also one of the countries with the most varieties of sturgeon and has a natural advantage in developing caviar products. [0003] In Iran, where caviar is eaten more, boric acid used to be a traditional raw material for making caviar. But boric acid is forbidden ...

Claims

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Application Information

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IPC IPC(8): A23L3/3517A23L3/3562A23L17/30
Inventor 石玉刚朱云洁
Owner ZHEJIANG GONGSHANG UNIVERSITY
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