Controllable biodegradable copolymer with basic copolymerization center
A biodegradable and copolymer technology, applied in the field of tissue engineering and polymer materials, can solve the problems of reducing the mechanical properties of materials, reducing the pH value of tissues, and adversely affecting the health of patients, achieving adjustable relative molecular weight, reducing expansion rate, improving Effects of mechanical strength and flexibility
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preparation example Construction
[0053] The preparation method of the basic copolymerization center is as follows: dissolving carboxypolyethylene glycol in deionized water, then adding EDCI to the above solution for 45 minutes of reaction; adding amino acid into the activated carboxypolyethylene glycol solution, and using 1M HCl Adjust the pH value to 4, continue the reaction at room temperature for 2 hours, and then the pH value rises to 6 and continue the reaction for 1 hour; the product is dialyzed in 1 mM hydrochloric acid solution at 10°C, repeat the dialysis twice (add 1% sodium chloride to the dialysate), and freeze-dry .
[0054] The preparation method of the copolymer: mix the basic copolymerization center, left-handed, right-handed lactide, and caprolactone, and add them to the reactor. After mixing, repeatedly vacuumize and fill with nitrogen for 20 times; Add a ring-opening polymerization catalyst to the reactor, heat the reactor to the corresponding temperature, and react for a certain period of ...
Embodiment 5
[0063] The tensile property research of embodiment 5 copolymers
[0064] Table 3 copolymer performance and its tensile performance comparison
[0065]
[0066] Lactide L / D in the above table refers to the feed ratio when L-lactide and D-lactide are polymerized
[0067] The detection of polymer intrinsic viscosity is based on GB / T 1632.1-2008 plastics, using a capillary viscometer to measure the viscosity of dilute polymer solutions, and the tensile strength and elongation at break are based on GB / T 1040.3-2006 Determination of tensile properties of plastics No. 3 Part: The test conditions for thin plastics and thin sheets are carried out. Dispersion is determined by GPC. As can be seen from Table 5, in 3 kinds of copolymers provided by the present invention, add multi-arm copolymerization center, PEG compared with non-added copolymer (comparative example 1,3), elongation at break has obvious increase, illustrates that the present invention provides The copolymer has high...
Embodiment 6
[0068] Example 6 Cytocompatibility and degradation solution pH of basic copolymerization center copolymer
[0069] 6.1 Cytocompatibility experiments of copolymers
[0070] The copolymers in Examples 1-4 and Comparative Examples 3-4 were hot-pressed into a film with a thickness of 0.2mm at 150°C and 9MPa with a flat bed, and then cut into discs with a diameter of 15mm, according to The sequence of ethanol, tap water and three-distilled water was washed three times repeatedly, and then washed three times with sterilized three-distilled water. After washing, it was fixed on the bottom of the well of the tissue culture plate, and after ultraviolet sterilization, 1 mL of cells was added to each well of the tissue culture plate. Suspension (1×10 5 cells / mL), at 37°C, 5% CO 2 After cultivating in an incubator with saturated humidity for a certain period of time, observe the cell morphology with a microscope, then remove the culture medium, wash away non-adherent cells with buffer, ...
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Abstract
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