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Pretreatment method and fermentation process of mixed lignocellulose

A technology of lignocellulose and fermentation process, which is applied in the field of mixed lignocellulose pretreatment method and fermentation process thereof, can solve the problems of affecting the process of fermented products, increasing the cost of subsequent processing, and high biomass slurry, saving chemical reagents, The effect of reducing the economic risk of enterprises and promoting the fermentation process

Inactive Publication Date: 2019-07-05
NANJING UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, the pH of the biomass slurry produced by these two pretreatments is too high or too low, so a large amount of acid and alkali needs to be added to adjust the pH to a range suitable for subsequent enzymatic fermentation, which will increase additional subsequent processing costs
At the same time, a large amount of salt will be introduced in the pH adjustment of the pretreated slurry, which will affect the subsequent enzymatic hydrolysis and fermentation to prepare fermented products such as ethanol.

Method used

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  • Pretreatment method and fermentation process of mixed lignocellulose
  • Pretreatment method and fermentation process of mixed lignocellulose
  • Pretreatment method and fermentation process of mixed lignocellulose

Examples

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Embodiment 1

[0034] After mixing various lignocellulose raw materials and performing pretreatment, lignocellulose slurry is obtained.

[0035] The present embodiment uses crop biomass as raw material to carry out the technique of ethanol fermentation according to the following steps:

[0036] (1) Biomass mixing. Corn, wheat and rice stalks were mixed and added into the reactor so that the substrate mass concentration was 10%, sodium hydroxide solution (2% w / w) was added, and pretreated at 121° C. for 20 minutes. Use hydrochloric acid to adjust the pH to 6-7, place the pretreated slurry in an oven, and dry the water at 60°C to 10-20% for use;

[0037] (2) Hydrolysis of mixed biomass. Add the pretreated slurry into the reactor, the substrate mass concentration is 10%, adjust the pH to 4.7-4.9, add Ctec 2 (Beijing Novozymes) enzyme, and carry out in a shaking box (250rpm) at 50°C enzymatic reaction;

[0038] (3) Ethanol fermentation. Add 60ml of enzymatic hydrolysis solution to a 150ml r...

Embodiment 2

[0040]Mix corn, wheat and rice stalks into the reaction kettle, add dilute sulfuric acid solution, so that the mass concentration of the straw substrate is 10%, and the sulfuric acid concentration is 1%. Pretreat at 160°C for 10 minutes, use sodium hydroxide to adjust the pH to 6.0-7.0, then place the pretreated slurry in an oven, and dry the water at 60°C to 10%-20%. The mass concentration of the substrate is 10%, the pH is adjusted to 4.6-7.0, and Ctec 2 Novozyme enzyme is added. Adjust to pH=5.5-6.5 after enzymatic hydrolysis, add yeast powder and peptone, according to the initial OD 600 =1.0-2.0 The bacteria of Zymomonas mobilis 8b were added, and the fermentation was carried out in a shaking box (150 rpm) at 30°C.

Embodiment 3

[0042] Firstly, one kind of lignocellulose raw material is subjected to acid pretreatment or alkaline pretreatment, and then different kinds of lignocellulose are sequentially added in batches to obtain a pretreatment slurry of mixed lignocellulose.

[0043] In this embodiment, poplar wood with a dense structure is added to the reactor for pretreatment, and then easily degradable corn stalks are added. Dilute sulfuric acid was added to make the substrate mass concentration 10%, and pretreatment was performed under the optimum reaction conditions for pretreatment. The pH of the pretreatment slurry was then adjusted to 6.0-7.0 using hydrochloric acid / sodium hydroxide. The pretreated mixed slurry is placed in an oven, and dried at 60°C to a moisture content of 10-20% for use. The mass concentration of the substrate is 10%, the conditions are pH=4.6-7.0, and Ctec 2 Novozymes enzyme is added to carry out the enzymatic hydrolysis reaction. After enzymatic hydrolysis, adjust the sl...

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Abstract

The invention discloses a pretreatment method and a fermentation process of mixed lignocellulose. The pretreatment method of mixed lignocellulose comprises the following steps: pre-treating lignocelluloses by mixing different lignocelluloses as a raw material, or after pre-treating the lignocelluloses, mixing acidic slurry with alkaline slurry or mixing the slurry of a low-concentration inhibitorwith the slurry of a high-concentration inhibitor according to the acidity and alkalinity of the slurry or the concentration of the inhibitor. The method utilizes mixed lignocellulose as a raw material and combining the advantages of different lignocelluloses, relieves problems of higher concentration of phenolic inhibitor caused by biomass with higher lignin content, can promote the fermentationprocess by mixing the lignocellulose with the biomass with richer nutrient substances at the same time, improves the yield of fermentation products such as ethanol, reduces the addition of additionalchemical reagents, and reduces the adverse influence of the inhibitor on fermentation.

Description

technical field [0001] The invention belongs to the technical field of biomass energy regeneration, and in particular relates to a mixed lignocellulose pretreatment method and a fermentation process thereof. Background technique [0002] As a renewable resource, lignocellulose can effectively alleviate environmental pollution and energy crisis caused by the utilization of fossil resources. Lignocellulose is widely distributed and mainly composed of cellulose, hemicellulose and lignin. Due to the dense structure of lignocellulose, it is usually necessary to undergo a pretreatment step to open the structure, making it easier to convert lignocellulose into fermentable sugar through enzymatic hydrolysis, and then further ferment to produce fermented products such as ethanol. [0003] However, the distribution and yield of biomass are affected by factors such as seasons, regions, and people's planting preferences. These factors will increase the cost of storage and transportati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/02C12P7/10D21C5/00
CPCC12P19/14C12P19/02C12P7/10D21C5/00C12P2201/00Y02E50/10
Inventor 金明杰陈相雪翟睿
Owner NANJING UNIV OF SCI & TECH
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