Method for detecting hydrogen peroxide and cysteine by using Au@NH2-MIL-125 as nano-enzyme catalyst
A cysteine and hydrogen peroxide technology, applied in the field of biological analysis, can solve the problems of inability to detect analytes, cumbersome, complex analysis and expensive biological instruments, etc., and achieve the effects of good stability, simple process method and mild synthesis conditions.
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Embodiment 1
[0035] Example 1 Preparation of Au@NH by impregnation-reduction method 2 -MIL-125 Nano Enzyme Catalyst
[0036] S1. 2-aminoterephthalic acid (0.543 g, 3 mmol) was dissolved in a solution containing DMF (9 ml) and MeOH (1 ml). Then tetrabutyl titanate Ti(OC 4 h 9 ) 4 (0.26ml, 0.75mmol) was added to the solution. The above mixture was sonicated for 10 min at room temperature, then transferred into a 50 ml Teflon liner and heated at 150 °C for 72 h. Finally, the obtained yellow powder was collected by centrifugation, washed with DMF and MeOH, respectively, and dried under vacuum at 60 °C;
[0037] S2. Synthesis of Au@NH by ultrasonic method 2 -MIL-125. First, under the action of ultrasound, 5mg NH 2 -MIL-125 dissolved in 5.76ml of water. Second, 120 μL HAuCl with a concentration of 1 wt% 4 , and kept under ultrasound for 5 minutes. Finally, 360 μL of trisodium citrate was quickly added to the solution and sonicated for 20 min until the color of the solution became a st...
Embodiment 2A
[0038] Example 2Au@NH 2 -MIL-125 Nano Enzyme Catalyst for Detection of Hydrogen Peroxide
[0039] The Au / NH prepared in Example 1 was evaluated by colorimetry 2 -The effect of MIL-125 on the detection of hydrogen peroxide, the test results are as follows figure 2 shown.
[0040] Take 20 μL TMB (15 mmol) and 20 μL Au@NH 2 -MIL-125 (3 mg / mL) was added to 3 mL of acetate buffer solution (pH=4.0). Add different concentrations of hydrogen peroxide into the above buffer solution. After a period of time, the color of the solution is light blue or blue, and the change of absorbance at 651nm is detected to make H 2 o 2 Concentration-dependent standard curve, quantitative detection of hydrogen peroxide in the analyte through the standard curve.
Embodiment 3A
[0041] Example 3Au@NH 2 -MIL-125 Nanozyme Catalyst for Detection of L-Cysteine
[0042] Colorimetric detection of L-cysteine:
[0043] Dissolve TMB in 15mM DMSO to prepare TMB solution, take 20μL TMB solution, 20μL Au@NH 2 -MIL-125 (3mg / mL), 20μL H 2 o 2 (30 wt%) was added to 3 mL of acetate buffer solution (pH=4.0). Add different concentrations of L-cysteine to the above buffer, and after a period of reaction, the different concentrations of cysteine will change from high to low, from colorless to light blue to dark blue. The L-cysteine-dependent standard curve was made by detecting the change of absorbance at 652 nm by UV-visible absorption spectrum. Quantitative detection of L-cysteine in the analyte was carried out through the standard curve.
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