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Hydrogel microneedle patch for collecting skin interstitial fluid and preparation method and application method thereof

A hydrogel and microneedle patch technology, applied in application, inoculation and ovulation diagnosis, medical science, etc., can solve the problems of limiting the instant diagnosis properties of microneedle patch, unable to realize instant application, not very good, etc., and achieve good repeatability. Freeze-thaw and reversible cross-linking properties, efficient recovery, effect of increasing swelling capacity

Active Publication Date: 2019-08-06
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the challenge of how to effectively recover the extracted interstitial fluid from the hydrogel microneedle patch is still not well resolved.
Although some of the skin interstitial fluid can be recovered from the microneedle patch by using high-speed rotation of the centrifuge and applying pressure, these methods require the use of large laboratory instruments, which limit the immediate diagnostic properties of the microneedle patch and make it impossible Realize instant applications in communities, homes, remote areas, etc.

Method used

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  • Hydrogel microneedle patch for collecting skin interstitial fluid and preparation method and application method thereof
  • Hydrogel microneedle patch for collecting skin interstitial fluid and preparation method and application method thereof
  • Hydrogel microneedle patch for collecting skin interstitial fluid and preparation method and application method thereof

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preparation example Construction

[0030] The preparation method of the hydrogel microneedle patch for collecting skin interstitial fluid disclosed by the present invention comprises:

[0031] 1) mixing polyvinyl alcohol (PVA) with another highly hydrophilic polymer solution;

[0032] 2) Pouring the mixed solution in step 1) into a microneedle mold, repeatedly freezing and thawing to form PVA cross-linked ice crystals, and demoulding after drying to form a hydrogel microneedle patch.

[0033] Preferably, the polyvinyl alcohol involved in step 1) has a molecular weight of 27,000-186,000 and a concentration of 5%-50%; highly hydrophilic polymer solutions are natural and synthetic: natural highly hydrophilic polymers Including polysaccharides (starch, cellulose, alginic acid, hyaluronic acid, chitosan, etc.) and polypeptides (collagen, poly L-lysine, poly L-glutamic acid, etc.), synthetic high hydrophilic Polymers include polyethylene oxide, acrylic acid and its derivatives (polyacrylic acid, polymethacrylic acid...

Embodiment 1

[0046] A method for preparing a hydrogel microneedle patch for collecting skin interstitial fluid, comprising:

[0047] 1) It is formed by mixing polyvinyl alcohol (molecular weight 27000, mass fraction 15%) and chitosan solution at a volume ratio of 1:0.25 to prepare a mixed solution;

[0048] 2) The mixed solution is poured into a negative mold made of polydimethylsiloxane (such as figure 1 (shown), vacuumize the mixed solution into the mold hole, freeze and thaw repeatedly to cross-link the hydrogel to form microneedles, and dry the hydrogel microneedles in a blast drying oven to obtain a hydrogel microneedle patch, as figure 2 shown.

[0049] When in use, the prepared hydrogel microneedle patch is pierced into the skin, and pressed for 1-10 minutes after piercing the skin. After taking it out, add a small amount of phosphate buffer (pH=6.8), heat in a water bath to dissolve the microneedles and release the target glucose in the skin interstitial fluid, take out the solu...

Embodiment 2

[0051] A method for preparing a hydrogel microneedle patch for collecting skin interstitial fluid, comprising:

[0052] 1) A mixed solution is prepared by mixing polyvinyl alcohol (molecular weight 61000, mass fraction 10%) and hyaluronic acid solution at a volume ratio of 1:1;

[0053] 2) Pour the mixed solution into the female mold, centrifuge to make the mixed solution enter the mold hole, freeze and thaw repeatedly to crosslink the hydrogel, and dry it in a blast drying oven to prepare the hydrogel microneedle patch.

[0054] When in use, the prepared hydrogel microneedle patch is pierced into the skin. Press for 1-10 minutes after piercing the skin. After taking out the microneedle, add a small amount of phosphate buffer solution (pH=7.2), hold the test tube wall tightly with both hands, and heat it with body temperature until the tip of the microneedle is completely dissolved in the buffer solution to release the target chloride ion in the interstitial fluid of the skin...

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Abstract

The invention discloses a hydrogel microneedle patch for collecting skin interstitial fluid and a preparation method and an application method thereof, and belongs to the technical field of biomedicaldiagnosis. The preparation method comprises the steps: 1) according to the volume ratio of polyvinyl alcohol to a high-hydrophilcity polymer solution of 1:(0.2-1), mixing polyvinyl alcohol and the high-hydrophilcity polymer solution, to obtain a mixed solution; and 2) pouring the mixed solution into a micro-needle mould, freezing and thawing repeatedly to form cross-linked polyvinyl alcohol ice crystals, then drying, and demoulding to obtain the hydrogel micro-needle patch. When in use, the hydrogel microneedle patch extracted with the skin interstitial fluid is immersed in a buffer solutionand is heated up to a proper temperature, and the cross-linked ice crystals of the hydrogel microneedle patch are dissolved in the buffer solution to release a target substance. The hydrogel microneedle patch is reasonable in formula composition, simple in preparation method, convenient to use, low in equipment requirements, and easy to operate; and the skin interstitial fluid can be quickly and efficiently recovered.

Description

technical field [0001] The invention belongs to the technical field of biomedical diagnosis, and relates to a hydrogel microneedle patch for collecting skin interstitial fluid, a preparation method and an application method thereof. Background technique [0002] Point-of-care diagnosis is a new type of method that can be analyzed immediately at the sampling site, eliminating the need for complicated processing procedures during laboratory testing, and quickly obtaining test results. The collection of test samples is the crucial first step in point-of-care testing, and the quality of test sample collection determines the accuracy and sensitivity of the test. The body fluid samples currently used in clinical testing include blood, urine, saliva and sweat. Because blood is rich in biological information, it is relatively easy to collect, but collecting blood will cause damage to the body and cause pain. Repeated collection of blood is prone to wound infection, causing psycholo...

Claims

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Application Information

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IPC IPC(8): A61B10/00
CPCA61B10/0045A61B2010/008
Inventor 徐峰何榕晏李菲牛艳
Owner XI AN JIAOTONG UNIV
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