Preparation method of nanocomposite with molecular targeting/gene/photothermal therapy
A nanocomposite, molecular targeted drug technology, applied in gene therapy, medical preparations with non-active ingredients, and medical preparations containing active ingredients, etc., can solve the problems of complexity, inability to meet clinical needs, tumor recurrence, etc. , to inhibit proliferation, achieve high-efficiency treatment, and improve the effect of treatment
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0045] 20 mg of N-4-azido-phthalimido-chitosan CS-N 3 Add to 2 mL dimethyl sulfoxide, stir until completely dissolved, then add 4 mg Er, 16 mg Q-amine, and 4 mg Cy7 to the flask. The above system was placed in a nitrogen atmosphere, and 200 μL of copper sulfate pentahydrate aqueous solution (20 mg / mL) and 200 μL of vitamin C sodium aqueous solution (15 mg / mL) were sequentially added dropwise to the flask with a 1 mL syringe. Cover the flask with foil and stir at room temperature for three days. The reaction solution was taken out and put into a dialysis bag (8000~14000 Da) for dialysis for three days. The dialysate was removed and lyophilized to obtain CE7Q. with ddH 2 O Make CE7Q and CQ into 460 μg / mL and 200 μg / mL solutions, respectively, then pipette 50 μL of each into a centrifuge tube, mix well to obtain CE7Q / CQ solution, and pipette with a pipette in a vortex state 100 μL of SV (80 μg / mL) solution was slowly added dropwise to the CE7Q / CQ solution and incubated at roo...
Embodiment 2
[0049] with ddH 2 o Make CE7Q and CQ in Example 1 into solutions of 100 μg / mL and 200 μg / mL, respectively, then draw 50 μL of each into a centrifuge tube, and mix well to obtain CE7Q / CQ solution. Pipette 100 μL of SV (10 μg / mL) solution into the CE7Q / CQ solution slowly dropwise, and incubate at room temperature for half an hour to obtain the nanocomposite CE7Q / CQ / S.
Embodiment 3
[0051] with ddH 2 O The CE7Q and CQ in Example 1 were prepared into solutions of 1000 μg / mL and 200 μg / mL, respectively, and then 50 μL of each was drawn into a centrifuge tube, and the CE7Q / CQ solution was obtained after mixing evenly. Pipette 100 μL of SV (300 μg / mL) solution slowly dropwise into CE7Q / CQ solution, and incubate at room temperature for half an hour to obtain nanocomposite CE7Q / CQ / S.
PUM
| Property | Measurement | Unit |
|---|---|---|
| particle diameter | aaaaa | aaaaa |
| particle diameter | aaaaa | aaaaa |
| particle diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More