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Biological decomposition agent and application thereof in compost

A decomposing agent and biological technology, which is applied in the field of screening and application of functional microorganisms, can solve the problems of shortening the decomposing time of compost, shortening the start-up period of compost, and unable to prolong the high temperature period, so as to improve the decomposing degree and fertilizer efficiency, reduce the morbidity rate, The effect of prolonging the duration

Active Publication Date: 2019-08-13
QINGDAO LILIHUI BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most of the currently commercially available decomposing inoculants are mesophilic microorganisms. Although they can shorten the start-up period of composting and make compost quickly enter the high-temperature period, they cannot prolong the high-temperature period and accelerate the decomposition of substances, because the inoculation is mesophilic. Microorganisms die rapidly during the high temperature period, therefore, shortening the time for the whole compost to mature is very limited

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  • Biological decomposition agent and application thereof in compost
  • Biological decomposition agent and application thereof in compost
  • Biological decomposition agent and application thereof in compost

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Screening and Identification of High-temperature-resistant Lactic Acid Bacteria

[0041] 1.1 Screening of high temperature resistant lactic acid bacteria

[0042] Sample: Bottom sludge of a biogas digester in a farm in Liaolan Town, Pingdu, Qingdao City, Shandong Province.

[0043] Sludge samples were subjected to high temperature enrichment culture at 70°C, and 55 high temperature resistant pure strains were isolated by gradient dilution method. The isolated bacterial strains were inoculated to the MRS plate adding 0.5% calcium carbonate respectively (peptone 10g, yeast extract 5g, beef extract 10g, glucose 20g, dipotassium hydrogen phosphate 2g, ammonium citrate 2g, sodium acetate 5g, magnesium sulfate 0.58g , manganese sulfate 0.25g, Tween 80mL, agar 15g, distilled water 1.0L, pH6.5±0.2), cultured at 37°C for 48h, isolated and cultured the strains with transparent circles, and obtained 3 strains of lactic acid bacteria, named LLH-1 respectively , LLH-2, L...

Embodiment 2

[0056] Example 2 Lactobacillus plantarum LLH-2 protease activity assay

[0057] Insert Lactobacillus plantarum LLH-2 into MRS liquid medium (peptone 10g, yeast extract 5g, beef extract 10g, glucose 20g, dipotassium hydrogen phosphate 2g, ammonium citrate 2g, sodium acetate 5g, magnesium sulfate 0.58g, manganese sulfate 0.25g, Tween 801mL, distilled water 1.0L, pH6.5±0.2), cultured at 37°C for 24h, successively transferred and activated for two generations, and inserted into 200mL MRS liquid culture with 1% ( / v / v) inoculum culture medium at 37°C for 48h; centrifuge at 8000r / min for 10min at 4°C, collect the supernatant, and detect the protease activity in the supernatant by the following method.

[0058] The results show that the protease activity in the fermentation supernatant of Lactobacillus plantarum LLH-2 screened by the present invention is as high as 97U / mL, and unexpected technical effects have been achieved.

[0059] (1) Definition of enzyme activity: at 37°C, the am...

Embodiment 3

[0063] Embodiment 3 Lactobacillus plantarum LLH-2 is used as decomposing agent in chicken manure composting

[0064] 1. The compost materials are chicken manure and sawdust, which are respectively taken from a chicken farm and a wood processing factory in Pingdu, Qingdao.

[0065] 2. Experimental group settings:

[0066] (1) Blank control group: inoculated with sterile water;

[0067] (2) CGMCC1.572 treatment group: inoculated with Lactobacillus plantarum CGMCC1.572;

[0068] (3) LLH-2 treatment group: Lactobacillus plantarum LLH-2 was inoculated.

[0069] According to the properties of chicken manure and sawdust, adjust the water content to about 60%, adjust the C / N ratio to between 25 and 30, mix well and put it into a foam box with a volume of 4L.

[0070] Before the compost inoculation, the Lactobacillus plantarum fermentation broth ((10 8 -10 9 cfu / ml)) Fully mix 5‰ of the wet weight of the material with cornmeal at a ratio of 1:1 by volume and mass, and inoculate it...

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Abstract

The invention relates to the technical field of function microbial screening and application, and particularly provides a biological decomposition agent and a preparation method and application thereof. According to the biological decomposition agent, by means of the cooperative promoting function of heat-resisting lactobacillus plantarum CCTCC NO:M2019401 and multiple kinds of microorganisms, thecompost temperature can be effectively improved, the high temperature period can be prolonged, the composting time can be shortened, and wide application prospects are achieved.

Description

technical field [0001] The invention relates to the technical field of screening and application of functional microorganisms, in particular to a biological decomposing agent and its application in composting. Background technique [0002] Composting is currently the most commonly used method for the harmless treatment of waste organic materials such as livestock and poultry manure, crop straw, domestic garbage, and urban sludge. The traditional composting method generally adopts the method of increasing nutrition and improving environmental conditions, and uses the indigenous microorganisms in the composting raw materials to degrade organic pollutants. However, due to the small amount of indigenous microorganisms in the initial stage of composting, it takes a certain time to reproduce, and various Microbial decomposition rates vary greatly, so traditional compost often has problems such as long fermentation time, odor and low fertilizer efficiency. Modern composting techno...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C12N1/14C05F11/00C05F17/00C12R1/25C12R1/125C12R1/465C12R1/72C12R1/885
CPCC12N1/20C12N1/16C12N1/14C05F5/00C05F11/08C05F17/20Y02W30/40
Inventor 代庆海孙春龙王丽宁王凌云李倩倩张峰
Owner QINGDAO LILIHUI BIOTECH
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