Preparation method of mulberry polysaccharide-iron chelate with anti-oxidation activity

A technology of antioxidant activity, mulberry polysaccharide, applied in the directions of antitoxin, drug combination, blood diseases, etc., can solve the problems of insufficient preparation and purification of polysaccharide, high cost of ethanol production, and inability to eliminate influence, etc., to achieve mass production, cost Low, improved absorption effect

Inactive Publication Date: 2019-08-16
珠海中美普莱健康科技有限公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the invention uses ethanol to wash and the production cost is high, and the degree of purification of polysaccharide preparation is not enough, and the influence caused by the combination of other components and iron cannot be ruled out.
[0006] At the same time, the function of polysaccharide-iron complex in the prior art is relatively single, and it is mainly used for iron supplementation, but at the same time, it has relatively few other functions and has a single purpose.

Method used

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  • Preparation method of mulberry polysaccharide-iron chelate with anti-oxidation activity
  • Preparation method of mulberry polysaccharide-iron chelate with anti-oxidation activity
  • Preparation method of mulberry polysaccharide-iron chelate with anti-oxidation activity

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Embodiment 1

[0035]The preparation method of Sevage reagent described in step (2) in embodiment 1, embodiment 2 and embodiment 3 is that chloroform, n-butanol are mixed by volume ratio 4: 1, and the method of Sevage method deproteinization is polysaccharide and Sevage reagent Mix at a volume ratio of 1:4, shake for 20 minutes, centrifuge at 5000r / min for 5 minutes, take out the polysaccharide solution in the upper layer, and repeat the above steps for 10 to 13 times until the protein is completely removed.

[0036] Example 1

[0037] (1) Extraction of mulberry polysaccharides: After 500 g of mulberries were dried naturally, they were refluxed with 95% ethanol at 70° C. for 4 hours to remove small molecular impurities such as lipids and pigments. Extract in a water bath at 90° C. for 2 hours, twice in total. After concentrating the water extract, add 80% ethanol for alcohol precipitation to obtain crude polysaccharide.

[0038] (2) Separation of mulberry polysaccharides: After removing pro...

Embodiment 2

[0043] (1) Extraction of mulberry polysaccharides: After 500 g of mulberries were dried naturally, they were refluxed with 95% ethanol at 70° C. for 4 hours to remove small molecular impurities such as lipids and pigments. Extract in a water bath at 90° C. for 2 hours, twice in total. After concentrating the water extract, add 80% ethanol for alcohol precipitation to obtain crude polysaccharide.

[0044] (2) Separation of mulberry polysaccharides: After removing protein from mulberry polysaccharides by Sevage method, the macroporous resin AB-8 was used to decolorize the polysaccharides by static adsorption, and the solution was clarified by shaking at 400 rpm at room temperature. The supernatant was concentrated and dialyzed against distilled water for 72h. Finally, 40% ethanol was added for ethanol precipitation, 5000r / min, centrifuged for 5min, the supernatant was discarded, the precipitate was dissolved in distilled water, and the obtained solution was freeze-dried to obtai...

Embodiment 3

[0050] (1) Extraction of mulberry polysaccharides: After 500 g of mulberries were dried naturally, they were refluxed with 90% ethanol at 90° C. for 5 hours to remove small molecular impurities such as lipids and pigments. The water bath was extracted at 70° C. for 4 hours, twice in total. After the water extract was concentrated, 40% ethanol was added for alcohol precipitation to obtain the crude polysaccharide.

[0051] (2) Separation of mulberry polysaccharides: After the mulberry polysaccharides were deproteinized by the Sevage method, the macroporous resin AB-8 was used to decolorize the polysaccharides by static adsorption, and the solution was clarified by shaking at room temperature at 500 rpm. The supernatant was concentrated and dialyzed against distilled water for 48 h. Finally, 20% ethanol was added for ethanol precipitation, 4500r / min, centrifuged for 10min, the supernatant was discarded, the precipitate was dissolved in distilled water, and the obtained solution ...

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Abstract

The invention discloses a preparation method of a mulberry polysaccharide-iron chelate with anti-oxidation activity. The preparation method comprises: carrying out ethanol reflux on mulberry, carryingout water bath extraction, and carrying out alcohol precipitation to obtain crude polysaccharide; removing proteins from the mulberry polysaccharide by using a Sevage method, decolorizing by using amacroporous resin, and shaking at a room temperature to clarify the solution; concentrating the supernatant, and dialyzing with distilled water; carrying out alcohol precipitation with ethanol, carrying out centrifugation, and carrying out freeze drying to obtain mulberry polysaccharide; dissolving the mulberry polysaccharide in water, purifying, collecting the eluent by using a NaCl solution as an eluant, concentrating, and carrying out freeze-drying to obtain mulberry polysaccharide for chelating; and dissolving the obtained mulberry polysaccharide and trisodium citrate in distilled water, adding an iron salt solution, carrying out a heating reaction, sequentially carrying out centrifugation and dialysis, carrying out alcohol precipitation with ethanol, and carrying out freeze-drying toobtain the mulberry polysaccharide-iron chelate. According to the present invention, with the method, the iron supplementing agent with anti-oxidation activity is prepared, and is easily absorbed by human body, such that the chelate provides the anti-oxidation effect while treats iron deficiency anemia so as to achieve double health care functions.

Description

technical field [0001] The invention relates to a preparation method of a polysaccharide-iron chelate with antioxidant activity, in particular to a preparation method of a mulberry polysaccharide-iron chelate with antioxidant activity. Background technique [0002] Iron is one of the essential trace elements for the human body. It is the core part of hemoglobin and myoglobin, and an important part of certain enzymes such as peroxidase. It is essential for the normal function of the human body, especially the metabolism and immune function. Iron-deficiency anemia (IDA) is one of the diseases with the highest incidence of nutritional deficiencies. Severe iron deficiency will lead to impaired enzyme function in cells, resulting in digestive tract and nervous system problems, which seriously affect human health. Oral iron supplementation is essential in the treatment and prevention of iron deficiency in humans. Ferrous sulfate has been widely used as an iron supplement for the ...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P39/06A61P7/06
CPCA61P7/06A61P39/06C08B37/00
Inventor 扶雄张佳琦陈春李超
Owner 珠海中美普莱健康科技有限公司
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