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Secondary salinized soil remediation bacterial agent and application thereof

A technology for secondary salinization and soil remediation, applied in the field of secondary salinization soil remediation inoculants, can solve the problems of soil secondary salinization and secondary pollution that cannot be fundamentally solved, and improve crop yield and food safety, reducing nitrate nitrogen content and improving soil fertility

Active Publication Date: 2019-09-10
漳州三炬生物技术有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The traditional ways to solve the secondary salinization of soil include: irrigation and salt washing method, soil amendment method, semi-decomposed organic fertilizer method and planting salt-tolerant plant varieties, etc. These methods are easy to cause secondary pollution and cannot fundamentally solve the problem of soil salinization. The secondary salinization problem of

Method used

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  • Secondary salinized soil remediation bacterial agent and application thereof
  • Secondary salinized soil remediation bacterial agent and application thereof
  • Secondary salinized soil remediation bacterial agent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1 A kind of secondary salinized soil remediation bacterial agent

[0042] 1. Medium preparation:

[0043] 1) Activation medium

[0044] Bacillus licheniformis: peptone 10g, beef extract 3g, NaCl 5g, agar 18g, distilled water 1L, pH7.0.

[0045] Paenibacillus jelly: 10g sucrose, 0.5g dipotassium hydrogen phosphate, 0.2g magnesium sulfate, 0.2g sodium chloride, 1g calcium carbonate, 20g agar powder (solid medium), 1L distilled water, pH 7.0.

[0046] 2) Fermentation medium:

[0047] Paenibacillus jelly: 5.5g starch, 1.579g yeast powder, 5.76g soybean meal powder, 1.4g magnesium sulfate, 2.0g dipotassium hydrogen phosphate, 8.5g calcium carbonate, 0.2g sodium chloride, pH 7.0.

[0048] Bacillus licheniformis: 15g sucrose, 10g soybean powder, 1g potassium dihydrogen phosphate, 0.1g zinc sulfate, pH 6.5.

[0049] 3) Expanded culture medium: the culture medium is the same as the fermentation medium.

[0050] 2. Bacterial liquid preparation:

[0051] 1) Activa...

Embodiment 2

[0057] Embodiment 2 A kind of secondary salinization soil remediation bacterial agent

[0058] 1. Medium preparation:

[0059] 1) Activation medium

[0060] Bacillus licheniformis: peptone 10g, beef extract 3g, NaCl 5g, agar 18g, distilled water 1L, pH7.4.

[0061] Paenibacillus jelly: 10g sucrose, 0.5g dipotassium hydrogen phosphate, 0.2g magnesium sulfate, 0.2g sodium chloride, 1g calcium carbonate, 20g agar powder (solid medium), 1L distilled water, pH 7.5.

[0062] 2) Fermentation medium:

[0063] Paenibacillus jelly: 5.5g starch, 1.579g yeast powder, 5.76g soybean meal powder, 1.4g magnesium sulfate, 2.0g dipotassium hydrogen phosphate, 8.5g calcium carbonate, 0.2g sodium chloride, pH 7.5.

[0064] Bacillus licheniformis: 15g of sucrose, 10g of soybean powder, 1g of potassium dihydrogen phosphate, 0.1g of zinc sulfate, pH7.0.

[0065] 4) Expansion culture medium: the culture medium is the same as the fermentation medium.

[0066] 2. Bacterial liquid preparation:

[...

Embodiment 3

[0073] Embodiment 3 bacterial strain produces enzyme activity situation

[0074] The fermented liquid fermented for 24 hours was taken, centrifuged at 12000 rpm for 15 minutes in a refrigerated centrifuge at 4°C to remove bacteria, and the supernatant (crude enzyme solution) was taken for enzyme activity detection. The crude enzyme solution inactivated at 100°C was used as the control for the blank.

[0075] The nitrate reductase activity in the fermentation broth of the bacterial strain is detected with reference to the methods of ROMANA.SIDDIQUI et al.: 50mM 2-morpholineethanesulfonic acid (MES, pH 6.5) buffer solution, 30mM potassium nitrate and 1mM sodium formate are preheated, at 37 Rapidly react with the same amount of fermentation broth at ℃, stop the reaction with the same amount of nitrite after 10 minutes, and measure at 436nm.

[0076] The nitrite reductase activity in the fermentation broth of the strain refers to the method of MARTINEZ-ESPINOSA RM et al. Mix 0.1M ...

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Abstract

The invention provides a secondary salinized soil remediation bacterial agent and application thereof, and belongs to the technical field of soil improvement. The bacterial agent is prepared from, byvolume, 0.5-1.5 parts of a bacillus licheniformis bacterial agent and 0.5-1.5 parts of a paenibacillus mucilaginosus bacterial agent; the living bacterium count of bacillus licheniformis in the bacillus licheniformis bacterial agent is 3-4 billion cfu / mL; the concentration of paenibacillus mucilaginosus in the paenibacillus mucilaginosus bacterial agent is 1.5-2.5 billion cfu / mL; the preservationnumber of the bacillus licheniformis is CGMCC No.10741; the preservation number of the paenibacillus mucilaginosus is CGMCC No.3995. The bacterial agent is utilized for remediating secondary salinizedsoil, the remediation effect is obvious, the content of nitrate nitrogen in the soil can be reduced, the soil fertility can be improved, and meanwhile, the yield and food safety of crops can be improved.

Description

technical field [0001] The invention relates to the technical field of soil improvement, in particular to a secondary salinized soil repair bacterial agent and its application. Background technique [0002] Facility agriculture is an important mode of production in modern agriculture. my country is a large country with facility agriculture, among which facility vegetables are the largest. Protected cultivation agriculture has played a huge role in the cultivation of vegetables and other important economic crops in my country, and has achieved significant economic and social effects. For example, today we can meet the needs of "eating summer vegetables in winter, winter vegetables in summer, western vegetables in Chinese, and southern vegetables in the north", mainly due to the development of my country's facility agriculture. However, with the excessive application of chemical fertilizers, as well as factors such as high temperature, high humidity, and lack of rainwater in...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C12R1/10C12R1/07
CPCB09C1/10C12N1/20
Inventor 郭小红刘玉珍黄娟朱春苗李肖宇骆毛喜林克明陈晓燕
Owner 漳州三炬生物技术有限公司
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