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Novel photothermal chemotherapy combined therapeutic reagent and preparation method and application thereof

A combined therapy and photothermal technology, which is applied in the direction of drug combinations, pharmaceutical formulas, medical preparations of non-active ingredients, etc., can solve the problems of easy oxidation, photothermal chemotherapy combined treatment reagents that have not been reported, and hinder the application of black phosphorus. Achieve high photothermal conversion efficiency, excellent tumor cell targeting and biocompatibility

Inactive Publication Date: 2019-10-01
SHANGHAI SIXTH PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

As a new type of two-dimensional material, black phosphorus has a high specific surface area, excellent electrochemical properties and good surface properties, which has laid a solid foundation for its application in many fields such as Raman detection, biomedicine, and chemical sensing. Solid foundation. Compared with other two-dimensional materials such as graphene, black phosphorus's relatively stable physical and chemical properties and high selectivity to some substances give it a great advantage in sensing. However, black phosphorus nano Tablets are easily oxidized in the air, resulting in insulating phosphorus oxide adsorbed on the surface of black phosphorus, which seriously hinders the application of black phosphorus in medicine
[0005] At present, functionalized graphene quantum dots have been reported as a photothermal agent, but functionalized graphene quantum dots coated with black phosphorus have not been reported as a combination therapy agent for photothermal chemotherapy

Method used

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  • Novel photothermal chemotherapy combined therapeutic reagent and preparation method and application thereof
  • Novel photothermal chemotherapy combined therapeutic reagent and preparation method and application thereof
  • Novel photothermal chemotherapy combined therapeutic reagent and preparation method and application thereof

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Effect test

Embodiment 1

[0047] A preparation method of a novel photothermal chemotherapy combination therapy reagent, specifically comprising the following steps:

[0048] (1) Preparation of black phosphorus nanosheets

[0049] S11. Add 25-50 mg of black phosphorus powder into a beaker containing 25-50 mL of N-methyl-2-pyrrolidone solution, place ice water in an ultrasonic instrument with an ultrasonic power of 300 watts, and place the beaker in an ice bath Ultrasonic oscillation for 8-10 hours to fully disperse;

[0050] S12. Centrifuge the dispersion at 4000 rpm for 8 minutes to remove residual unstripped particles; centrifuge the supernatant at 4000 rpm for 5 minutes to remove N-methyl-2-pyrrolidone, The precipitate after separation is black phosphorus nanosheets;

[0051]S13, dissolving the separated black phosphorus nanosheets in ethanol, storing them in ethanol to prevent oxidation of the black phosphorus nanosheets, and set aside;

[0052] (2) Preparation of graphene quantum dots with respo...

Embodiment 2

[0068] In Vitro Photothermal Properties of Graphene Quantum Dots / Black Phosphorus Nanoreagent Responsive in Second Infrared Region

[0069] Dilute the graphene quantum dots / black phosphorus nanoreagent to different concentrations (0-600 μg mL -1 ), and then transferred to a 1.5mL centrifuge tube, with a 1064nm laser at 0.6W cm -2 The power density was irradiated for 5 minutes, and the temperature change was recorded with an infrared thermal imager every 20s. Change power (0.4-0.8W cm -2 ) irradiate 600μg mL with a 1064nm laser -1 Graphene quantum dots / black phosphorus nanoreagents to determine the effect of different power on heating. Passing power density is 0.6W cm -2 The 1064nm laser was repeatedly irradiated by the temperature change for 5 minutes to explore the photostability of graphene quantum dots / black phosphorus nanoreagents.

[0070] Such as Figure 8 Shown is the infrared thermal imaging image of the graphene quantum dot / black phosphorus nano-reagent under th...

Embodiment 3

[0072] In Vitro Photothermal Therapy of Graphene Quantum Dots / Black Phosphorous Nanoreagents:

[0073] The invention provides the detection of the cell activity of the cells treated with the graphene quantum dot / black phosphorus nano reagent by the MTT method. Human cervical cancer cells (Hela) were planted in a 96-well plate at a density of 5,000 per well, cultured for 24 hours, and then added with different concentrations of graphene quantum dots / black phosphorus nano-reagents, cultured for 4 hours, and treated with 0.6W cm -2 The 1064nm laser was irradiated for 5 minutes, and then the cells were continued to be cultured for 24 or 48 hours. After the test time was reached, 20 μL of MTT was added, and then continued to incubate at 37°C for 4 hours. Dissolved with sulfoxide, and measured the absorbance at 490nm with a microplate reader.

[0074] Such as Figure 9 Shown, for graphene quantum dots / black phosphorus nanoreagent at 0.6W cm -2 The effect of 5 minutes of light on...

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Abstract

The invention discloses a novel photothermal chemotherapy combined therapeutic reagent and a preparation method and application thereof. The preparation method comprises the steps that (1), black phosphorus nanosheets are prepared; (2), near-infrared two-region-responsive graphene quantum dots are prepared; (3), the black phosphorus nanosheets and the near-infrared two-region-responsive graphene quantum dots are vigorously stirred in phosphate-buffered saline at room temperature for 24 hours, thus the near-infrared two-region-responsive graphene quantum dots are deposited on the surfaces of the black phosphorus nanosheets, centrifugal treatment is conducted, and the novel photothermal chemotherapy combined therapeutic reagent is obtained. According to the preparation method of the novel photothermal chemotherapy combined therapeutic reagent, by making the functionalized graphene quantum dots wrap the black phosphorus surface, the defect that the black phosphorus nanosheets are likely to be oxidized in air is overcome; the prepared novel photothermal chemotherapy combined therapeutic reagent has the high photothermal conversion efficiency, excellent tumor cell targeting and biocompatibility.

Description

technical field [0001] The invention relates to the technical field of nano-medicines, in particular to a novel photothermal chemotherapy combination therapy reagent and its preparation method and application. Background technique [0002] Cancer is still a major disease threatening human health in the past few decades. There are approximately 14 million new cancer patients worldwide each year, while 8 million people die from cancer-related diseases each year. As a promising alternative or supplementary therapy to traditional cancer treatment techniques (surgery, radiotherapy, and chemotherapy), combined photothermal chemotherapy therapy combines the synergistic effects of photothermal therapy itself and hyperthermia-induced The drug is released quickly, has a high curative effect on the inhibition of local tumor growth, and can avoid damage to normal tissues. As an effective non-invasive treatment, combined photothermal and chemotherapeutic therapy can treat many types of...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K47/69A61P35/00B82Y5/00B82Y30/00B82Y40/00A61K31/704
CPCA61K31/704A61K41/0052A61K47/6923A61P35/00B82Y5/00B82Y30/00B82Y40/00A61K2300/00
Inventor 沈龙祥耿弼江潘登余
Owner SHANGHAI SIXTH PEOPLES HOSPITAL
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